In view of the existing problems in the cultivation of professional core competence, according to the social demand for talent ability and starting from the concept of OBE, the “One body, two wings and three drives” type of food quality and safety professional core competence was constructed in this paper. “One body” referred to the main body of professional core competence, “whole-process control ability of food quality and safety”; “Two wings” referred to two important professional core competence “food quality control ability” and “food safety testing ability”; “Three drives” referred to the “food science” “professional quality” and “scientific” and “technological innovation” that is, the three aspects of knowledge, ability and quality as the driving force. From this, 2 first-level, 15 second-level and 15 third-level professional abilities were sorted out, professional core competence assessment system was developed and put into practice, and good results have been achieved.

Urea slow-release fertilizer is a new fertilizer to improve the utilization rate of nitrogen fertilizer, which is important for improving the utilization rate of nitrogen nutrients and crop yield. The application of sustained-release urea in the cultivation of grain crops such as wheat, corn, rice and economic crops such as cotton, soybean and so on in recent years is summarized in this paper. It is believed that the application of urea slow-release fertilizer in the process of crop planting has obvious effect of reducing nitrogen and increasing yield. At the same time, some problems still lacking research of urea sustained-release fertilizer in crop application are pointed out, such as the influence of slow-release urea fertilizer on the quality of crops, the influence of slow-release materials on environment and whether the slow-release curve matches the crop nutrient requirement curve. This study will provide some theoretical references for the future application research or widespread application of urea sustained-release fertilizer.

Green manure plants have high nutrient content. Overturning green manure crops is conducive to improving soil quality, cultivating good farming soil, and improving crop quality. It also has certain synergistic effects on improving soil physical and chemical properties, improving fertilizer utilization, and promoting crop growth. With the fresh green plant body as a fertilizer base, green manure is a natural green pollution-free fertilizer formed by directly or indirectly compressing fresh samples of the plant body into the soil to release nutrients into the soil after its decay and decomposition instead of fertilizer for the growth of the main crop, or by intercropping the green fertilizer crop with the main crop, to provide nutrients for the main crops, promote the growth and development of the main crops, and and improve the physical and chemical properties of the soil. In this paper, the characteristics of green manure crops were analyzed, and the effects of green manure crops on soil physical and chemical properties and fertilizer utilization rate were discussed. The future research directions and prospects of green manure were expected, indicating that green manure has great potential in agricultural development. The development potential is to provide reference for the research on the effects of green manure on crop production.

Taking water content and water activity as evaluation indexes, the optimum proportion of sodium lactate, glycerin, sorbitol and trehalose in preserved pork was determined by single factor and L ₉（3 ⁴）orthogonal test. The dried pork slice was prepared according to the formula and then stored at 20 ℃ for 0, 15, 30, 45, 90, 120, 150 and 180 days. The water content, water activity, red value, total bacteria and mould number of the dried pork slice were determined, and the quality changes during storage were analyzed. The results showed that when 0.16% sodium lactate, 2% glycerol, 0.015% sorbitol and 1% trehalose were added in the processing of preserved pork, the moisture content of the product was 16.79%, and the water activity of the product was 0.677. During storage at 20 ℃, the number of moulds and total bacteria accorded with the national standard. The moisture content, water activity and red value gradually decreased. The shelf life of the product was 90 days, which was determined by sensory evaluation. At this time, the moisture content was 25.8%, the water activity was 0.778, and the red value was 19.8.

Rapid detection is an important measure to ensure food safety for teachers and students in colleges and universities. The construction of rapid testing laboratory is of great significance for food safety and school stability in colleges and universities. This article analysed the background of rapid detection, the significance of detection results, detection items and future development direction. The purpose is to provide direction and ideas for the construction and development of rapid testing laboratories in colleges and universities.

With persimmon as raw material, the effects of different fermentation temperature, initial acidity, initial sugar content and yeast inoculation amount on the quality of persimmon wine were studied through single factor and orthogonal experimental design. The brewing process of persimmon wine was optimized by measuring residual sugar, total acid, alcohol content and sensory score in persimmon wine. The results showed that the optimum fermentation conditions of persimmon wine were as follows: fermentation temperature was 20 ℃, initial acidity was 7.5 g/L, initial sugar was 200 g/L, and yeast inoculation amount was 0.2 g/L. Under this optimal combination, the sensory score of persimmon wine was 92 points. The aroma components of persimmon wine with the best technology were analyzed by GM-MS. The aroma components of persimmon wine included 68 volatile substances, including 15 alcohols, 27 lipids, 7 acids, 6 aldehyde and keone, 7 alkanes and 6 others. The main aroma ingredients of persimmon wine include: Isoamyl alcohol（apple brandy and spicy aroma）, phenylpropanol（rose fragrance）, isoamyl acetate（pleasant banana aroma）, ethyl nonanoate（oil, fruit and brandy aroma）, 2-methylbutyric acid（pleasant fruit aroma）, and 3-hydroxy-2-butanone（milk aroma）, etc.

Plant pathology is a traditional subject of agricultural university. The development trend of Tianjin coastal urban agriculture puts forward higher requirements for the development of the course. Based on teaching reform in Tianjin Agricultural University, teaching reform of plant pathology course was explored from the aspects of teaching ideas, methods, content, objective, scientific research training, practice ability and mode of examination.

Cultivating distinctive university culture and promoting cultural education is an important task for building a socialist cultural power, and it is also an important guarantee for “Sanquan Education” in universities. Tianjin Agricultural University continues to implement the development strategy of “culture casting school” and takes cultivating the feelings of teachers and students who “love the CPC, love the motherland, love agriculture, and love the campus” as the goal and task of building morality and cultivating people. In practice, it proposes the school cultural logo of “Siyuan Fenjin”, constructs the cultural education work system of “Siyuan Fenjin”, and strives to cultivate new people of the era who shoulder the responsibility of national rejuvenation.

In order to have a clear view of the achievements and trends of residents recreation behavior, it is given an overall retrospect of development of residents recreation behavior research from our national journals based on the concepts of recreation, and it is also reviewed the recreation motivation, recreation decision-making, recreation fluidity of space-time, recreation satisfaction and the impact of recreation behavior. On the basis of the assessment, the relevant research implications involving in the research fields, research methods, research contents and research scales are put forward.

Accounting is a strong theoretical and practical subjects. Applied undergraduate accounting students' practice ability is especially concerned by employing units. In this paper, by establishmenting the core practice ability of students majoring in accounting, it expounds the applied undergraduate accounting professional three levels of core practice ability, and put forward the training measures which is consisted by the high-quality double-qualified teachers and practical teaching evaluation system to realization accounting professional core practice ability.

The environmental factors in water include biological factors and abiotic factors, and there are differences among ecological factors in different aquaculture water bodies. The environmental factors have direct or indirect effects on the quality of cultured fish. The improvement of the quality of cultured fish has always been concerned by researchers, but most of them focus on changing diet, and there are few studies on environmental factors. This paper briefly summarizes the ecological factors, the effects of ecological factors on fish quality and the evaluation of fish quality indicators, in order to provide references for this kind of research.

Cinnamaldehyde is the main active component of Cinnamomum cassia Presl, which has been studied and applied widely in terrestrial animals, but little in aquatic animals. This paper summarized the current embedding method of cinnamaldehyde and application in the aspects of anti-bacterial, anti-insect, food attraction and preservation of aquatic products, in order to provide reference for the research and application of cinnamaldehyde in aquatic animal health breeding and aquatic product preservation.

Bacillus velezensis, which is a new species of Bacillus, can promote plant growth and inhibit plant pathogens in agriculture and has great potential to be developed as a biocontrol agent. It can also be used in industry to degrade industrial harmful by-products and be widely used in biomedicine.Therefore, it has attracted much attention in recent years. In this paper, the discovery and classification, genome research, biological control and application of B. velezensis were reviewed in order to provide reference for further research and development.

Cinnamon oil is a kind of volatile oil component separated from cinnamon or cassia twigs. Its application in solution environment is limited by its poor water solubility and volatility. Thus, Cinnamon oil is prepared into emulsion in this study to improve its stability and water dispersivity. Firstly, centrifugal stability is taken as the evaluation index, and the suitable range of oil phase proportion, emulsifier and stabilizer dosage in emulsion prescription is determined through single factor screening. Then, the optimum formulation of Cinnamon oil emulsion is determined by orthogonal design with the retention rate of centrifugal emulsion as the evaluation index. In the optimal formulation, the mass ratio of Cinnamon oil, emulsifier Tween-80 and stabilizer xanthan gum is 10%, 2.0% and 1.5%, respectively. Under the optimal formulation, Cinnamon oil emulsion could be prepared with favorable stability and homogeneity. This study provides the basis for further application of Cinnamon oil emulsion.

Since the 18th National Congress of the Communist Party of China, General Secretary Xi Jinping emphasized that moral education should run through the whole process of education, and that all kinds of courses should go together with ideological and political courses to form synergistic effect. Taking Hydraulics as an example, this paper made a preliminary exploration into the ideological and political teaching of specialized courses. The ideological and political elements contained in Hydraulics were deeply explored, and the ideological and political education was integrated into the classroom teaching of hydraulics from the aspects of China’s achievements in Hydraulics, scientists’ deeds, typical water conservancy projects, life practice, engineering ethics and classical culture. In a silent way, the students were taught the socialist core values, academic aspirations and professional ethics. The teaching mode and carrier approach of hydraulics course were innovated and improved the college student’s acquisition sense of ideological and political education.

In this study, minced beef bone（the mass ratio of bone to meat is 3∶7）was used as raw material. Traditional, spicy and fermented beef flavor were prepared by hot-pressing extraction and enzymatic hydrolysis followed by Maillard reaction with adding potassium sorbate in beef flavor as control group. The pH value, water activity and brightness of beef flavor were measured during storage at 4 ℃ and 20 ℃. The results showed that the pH values of BF with spices were different from those of other groups, and the changes of brightness and water activity were the same among the six groups. The shelf life of the beef flavor can reach 2.5 months and 40 d at 4 ℃ and 20 ℃, respectively. After modification, the product was stratified obviously.

Malus sieversii is a tertiary relict plant. As a precious fruit germplasm, it has some excellent traits, such as stronger anti-cold ability, the resistance to insect, disease and drought. In this paper, the biological characteristics of seed germination in different populations of Malus sieversii were explored. The results show that 1 000-seed weight varied among different populations, and those of HR were the heaviest (26.86 g), subsequently with GY population (25.63 g), XY population (23.58 g) and NY population (19.76 g). By comparing three different methods of seed germination, it is concluded that the germination rate was much higher with removing the seed coat, reaching to 100% after 3 days. However, the seeds stratified outdoor at low temperature started to germinate after 40 days, with germination rate of 85.67%. When treated in freezer at 4 ℃, the seeds germinated after 60 days, with germination rate of 91.33%. The seed coat could be the main limiting factor for its germination. According to the study, it is can also be concluded that seeds stored for less than 3 years have higher germination rate of over 87%, seeds stored for 7 years dropped prominently to 31.6%. These results also show that germination rate in HR population was the highest (93%), followed by XY population, and that in NY population was the lowest (74%).

To find out an effective and simple method to make the casting specimen of rabbit’s pulmonary trachea and bronchial tree, so as to provide a real and rigorous anatomical basis for veterinary related teaching, scientific research and clinical practice, three corrosion methods were used in this research. Self-solidifying denture powder and denture water were used to prepare fillers in proportion, and then perfusion from the throat into the lungs of rabbits. After the filler solidified, Sodium hydroxide solution, sulfuric acid solution and hydrochloric acid solution were used to corrode the lung tissues. The results showed that all of the three corrosion methods could get good cast specimen. The specimen corroded by sodium hydroxide solution only needed to be immersed in the same solution again; The specimens corroded by sulfuric acid solution were black and needed to be bleached again; The specimens corroded by hydrochloric acid solution needed to be immersed in alkaline solution to remove the residual lipids. After filling the lung with self-coagulating dental powder and dental bracket water at the ratio of 1.0∶1.5, 50% sodium hydroxide solution were used to corrode the lung tissue. The intact, moderately elastic, clean and smooth respiratory cast specimens could be obtained, and bronchial trees could be clearly seen. Sodium hydroxide solution did not need to be equipped with other solutions, and the substance was not volatile. It is the most convenient and safe method among the three corrosion methods.

With annual grafted jujube seedlings (rootstocks were sour jujube) of two jujube varieties, ‘Yueguang' and ‘Fucuimi', as test materials, the growth and photosynthetic characteristics of the two jujube varieties in Tianjin were analyzed by measuring their heads, leaf growth indexes and photosynthetic indexes, with a view to provide references for their introduction and planting in Tianjin area. The results of the study showed that the length and thickness of the jujube heads of both jujube varieties showed a “double S-shaped” growth curve with two growth peaks throughout the growth period, both around the 18th day. ‘Yueguang' reached its second growth peak around the 65th day, and ‘Fucuimi' reached its second growth peak around the 60th day. Both varieties stopped growing around the 110th day（late August）. The annual growth rate of head length and thickness of ‘Fucuimi' was significantly higher than that of ‘Yueguang'. During the whole growth period, the average growth rate of length and thickness of jujube heads was higher in ‘Fucuimi' than in ‘Yueguang'. The leaf water content and leaf thickness of ‘Fucuimi' were significantly higher than ‘Yueguang'. The leaf shape index of ‘Yueguang' was significantly higher than that of ‘Fucuimi'. The photosynthetic indexes of both varieties were not significantly different. The comprehensive analysis concluded that ‘Fucuimi' is more adaptable than ‘Yueguang' in Tianjin area.

The chilling tolerance of 14 maize hybrids at germination and seedling growth stage were identified under room temperature and low temperature stress conditions. The relative values of each trait including germination rate, mean germination time, the height of shoot, the length of root, dry weight of shoot and root were used as indexes to assess chilling tolerance. The results show that the dry weight of root of maize hybrids with chilling tolerance didn’t decrease. Maize hybrids were clustered into three groups: chilling tolerance, intermediate chilling tolerance and chilling sensitivity. The chilling tolerance varieties included Zhengdan 958, Jiyuan 1 and Jundan 20, while Jinbei 288 and Gaoyou 1 belonged to chilling sensitivity varieties and the rest were ascribed to intermediate chilling tolerance varieties. The correlation analyses show that the relative values of shoot height, dry weight of shoot and root were suitable parameters for evaluating chilling tolerance.

The large spike-like membrane glycoproteins from the Newcastle disease virus envelope were encoded by Newcastle disease virus hemagglutinin-neuraminidase（HN）gene. HN protein is the abbreviated form of hemagglutinin-neuraminidase protein, it not only mediates virus adsorption on tumor cells, but also makes virus invade tumor cells with the help of the fusion protein, and its neuraminidase activity can hydrolyze surface sialic acid of the host cells and expose the biological recognition sites of host cells. It is also able to hydrolyze sialic acid lying on sugar chain end of virus particle surface, prevent virus particles from clustering on the host surface, furthermore, it can introduce new immunogenic factors and adhesion molecules to induce the recognition of immune systems.

Hard branches with buds, greenwoods with buds and leaves explants of salt tolerance Rosa rugosa var. of transgenosis were cultured on Murashige and Skoog (MS) basal medium supplemented with different concentrations of either 6-BA solely or in combination with naphthalene-3-acetic acid (NAA). The best response in terms of the percentage of shoot regeneration was obtained from greenwoods with buds cultured on MS medium supplemented with 0.5 mg/L 6-BA, whereas the highest number of shoots per responding explant was recorded on medium containing 0.5 mg/L 6-BA and 0.5 mg/L ZT. Successful rooting was achieved by placing the microshoots onto 1/2 MS medium containing 0.5 mg/L NAA. Of the various substrates tested, riversand∶vermiculite∶peat=1∶1∶1 was the best for plant acclimatization, as 85% of the plants survived.

：This paper compared the antioxidant activity of Angelica sinensis polysaccharide（ASP）before and after protein removing. Crude A.sinensis polysaccharide（CASP）was extracted with hot water and precipitated with ethanol. The ASP was obtained by removing protein from CASP by Sevag method. Ferricyanide reduction method, fenton method, DPPH method, and adjacent benzene three phenol autoxidation method were adopted to assay the reduction ability and free radical-scavenging activity in vitro. The results showed that CASP and ASP exhibited significant reduction ability and free radical-scavenging activity（ P<0.05）. Within the same concentration, the reduction ability and free radical-scavenging activity of CASP were higher than those of ASP（ P<0.05）. However, the reduction ability and free radical-scavenging activity of CASP and ASP were feebler than those of ascorbic acid（ P<0.05）. These results indicate that CASP and ASP exhibited antioxidant ability, the antioxidant ability of CASP was stronger than that of ASP.

To investigate the effects of miR-139 on myofibers of denervated muscle atrophy in mouse, anamyotrophia mouse model of sciatic nerve resection was established. 10 nmol miR-139 inhibitors were injected into the denervated gastrocnemius. 14 days after injection, the expression of miR-139 was detected by qRT-PCR, the wet weight and the cross section diameter of the muscle fibers were compared, and the expression of myosin heavy chain（MHC）was examined by RT-PCR and Western blot analysis between the atrophying skeletal muscle and control. The results showed that the cross section diameter of the muscle fibers in treated group was significantly higher than that of control group. The results suggested that miR-139 may play an important role in muscle growth and differentiation in vivo.

In order to screen sweet cherry cultivars for cultivation in saline-alkaline land of Tianjin, the relative membrane soil conditions of cherry leaf permeability, malondialdehyde（MDA）, soluble sugar, proline（PRO）content and peroxidase（POD）activity, superoxide dismutase（SOD）activity were studied with seedlings grafted by 3 cherry varieties named as Early American（AM）, Brooks（BRO）, Santina（SAN）on 2 kinds of root stock, and the seedlings were planted in neutral soil in Jixian County and saline-alkaline soil in Xiqing District. The results are as follows. In the saline-alkaline soil, the cytoplasmic membrane permeability of AM and BRO increased 30.0% and 73.3%, respectively. The MDA content of AM and SAN increased 50% and 15.6%, respectively. The soluble sugar content of SAN reduced 27.0%. The proline content of BRO increased 1.72 times. The POD enzyme activity of SAN increased 1.43 times. The proline content of AM reduced 79.0%. The SOD enzyme activity of BRO and SAN increased 3.33 times and 1.43 times, respectively. The SOD activity of AM decreased 46.6%. The membership function analyses show that the salt tolerance of 3 varieties, from high to low, is SAN, BRO, AM, therefore, SAN is suitable for planting in the saline-alkaline soil of Tianjin.

Type 2 diabetes is acommon chronic disease, and it is associated with insulin resistance. Loss or dysfunction of β-arrestin2 results in the development of insulin resistance and progression of type 2 diabetes. However, administration of β-arrestin2 significantly amend glucose intolerance and insulin resistance. In this article, We fused SUMO and β-arrestin2 gene with recombinant PCR methods, and then inserted it into pET22b vector, and constructed a expression vector pET22b- SUMO- β-arrestin2 successfully. A recombinant expression plasmid was expressed in Rosetta Blue（DE3）line of E. coli with IPTG at the temperature of 20 ℃. The SUMO-β-arrestin2 fusion protein was purified with Ni-NTA successfully. Lay the foundation for subsequent pharmacodynamic research of β-arrestin2.

An suspected porcine circovirus disease was confirmed by necropsy, histopathology observation, virus isolation and PCR detection, which was caused by porcine circovirus 2（PCV2） infection. A porcine circovirus strain（PCV2-JL/China/2015）was successfully isolated fromspleens and lymph nodesby passaging in PK-15 cells. A pair of primers based on PCV2 was designed according to the published sequences in GenBank. The length of complete genome of PCV2-JL/China/2015 was 1 767 bp by sequencing analysis. The results of the phylogenetic tree analysis indicated that there was a close genetic relationship between PCV2-JL/China/2015 and FJ594471-1C01 isolate from Changsha city.

The effects of LED (light emitting diode) with 30% R (red light)+70% B (blue light)and 70% R+30% B light ratios on growth and photosynthetic characteristics of cherry tomato seedlings were studied compared with the control group with 50% R+50% B. The results show that under the conditions of 70% R+30% B, the plant height, leaf area, fresh and dry weights, chlorophyll content and photosynthesis were all the maximum and significantly higher than those of 30% R+70% B and the control. Under the conditions of 30% R+70% B, the plant height, chlorophyll content and leaf area were the lowest, while the stem diameter was significantly higher than that of others. It is concluded that 70% R+30% B was better for the growth of cherry tomato seedlings.

It is described the main objectives, contents, teaching methods of the comprehensive experiment as well as the effects in Biochemistry and Molecular Biology Experiment. Through opening the course, the abilities of analyzing and solving the problems for students are greatly improved.

In this paper, a split-plot experiment was designed to study the effects of different planting densities and row spacing modes on filling rate and grain quality of a summer maize variety ZD958. Four planting densities were 93 000, 81 000, 69 000 and 57 000 plants/hm ², respectively. Three row spacing modes were one plant per spot with equal row, three plants per spot with equal row and three plants per spot with wide and narrow row, respectively. The results indicate that grain filling process did not significantly change with planting density increase while row spacing mode probably affected grain filling under the same planting density. Neither planting density nor row spacing significantly influenced crude protein and fat content, while higher planting density, such as 93 000 plants/hm ², could significantly decrease soluble sugar content and sucrose content. The cropping pattern of three plants per spot with wide and narrow row could significantly decrease sucrose content. Therefore, it was concluded that in order to increase the economic benefits of maize production, the planting density and row spacing mode should be adjusted in accordance with the requirement for yield or quality in practice.

It is analyzed the important position of occupation career planning and employment guidance in employment work for contemporary college students. And a scene simulation teaching mode should be introduced to the occupation career planning and employment guiding course is put forward, and the meaning, the characteristics, the common form of the scene simulation teaching mode are described. Also, this mode is introduced to the occupation career planning and employment guidance for the teaching arrangement. Finally, it is pointed out the detailed problems that teachers should pay attention to in the occupation career planning and employment guidance courses in using the scene simulation teaching mode.

This research was carried out from three aspects, including strain, inoculation and host, to study the conditions of Antheraea pernyi pupa infected by Cordyceps militaris（L.）Link. The result shows that add 70% leaching juice of the pupa into medium for activation of strain and inoculate to sterilized（121 ℃, 20 min）pupa by injection, can make the fruiting body of Cordyceps militaris（L.）Link achieve the best effect. The time of pupa become rigid can be shortened to 6 days and the rate of rigidity and germination can be increased to 98.0% and 99.3%, respectively.

In order to compare the physiological characteristics of different species of North American begonia on saline land in Tianjin, the soluble protein, soluble sugar, free proline, malondialdehyde content, membrane permeability, SOD, POD enzyme activity and other physiological and biochemical indexes in leaves were determined, with eight species of North American begonia as test material in Jinghai. The results showed that the highest soluble protein content was in Kells（42.235 mg/g）, the lowest was in Donghong（14.763 mg/g）; the highest soluble sugar content was in Kells（0.163%）and Julie（0.151%）, the lowest was in Wumei（0.103%）; the highest free proline content was in Kells（7.285 μg/g）and Donghong（6.211 μg/g）, the lowest was in Royal（2.747 μg/g）. The highest malondialdehyde content was in Kells（0.133umol/g）, the lowest was in Adam（0.079 μmol/g）and Julie（0.075 μmol/g）; the highest membrane permeability（34.96%）was in Donghong, the lowest was in royal（8.22%）; the highest POD enzyme activities was in Xiyangyang（50.771 U/g·min）and Wumei（54.254 U/g·min）, the lowest was in Donghong（12.208 U/g·min）; and there was no difference in SOD activity among the eight species of North American Begonia, and the enzyme activity was between 0.512 U/g and 0.613 U/g. According to the comprehensive analysis of membership functions, their order of saline tolerance were Kells>Donghong>Ruby>Julie>Adam>Royal>Xiyangyang>Wumei.

In order to explore the differences between blackberry and raspberry, the peel anatomy structure of blackberry and raspberry was researched. The results showed that: The cells of blackberry were oblong, and epicarp was smooth and hairless, while raspberry’s cells were irregular, and epicarp was covered with hairs; The cell size of blackberry epicarp was 0.973 μm ², 2.01 times than raspberry, which was 0.483 μm ²; And the cell size of blackberry mesocarp was 40.35 μm ², 3.55 times than raspberry, which was 11.37 μm ², besides the cell of blackberry mesocarp was more loosely arranged than raspberry; The cell size of blackberry endocarp was 18.09 μm, 1.48 times than raspberry, which was 12.20 μm. Moreover, the endocarp of blackberry and raspberry were both hardening and divided into three parts, the cell structure of each part was quite different from others, and the outer part formed different texture. Therefore, the differences of anatomy structure between blackberry and raspberry could be used as an important basis for species identification.

In this experiment, the acute toxicity effects of Chinese herbal medicines（quisqualis, sophora, ebony and cloves）on Cynoglossus semilaevis Gunther and Scuticoci liatlda were studied in lentic condition at room temperature. The results show that the safe concentration of the Chinese herbal medicines on Cynoglossus semilaevis Gunther was 0.030 8 g/L, the median lethal concentration of Chinese herbal medicines on Scuticoci liatlda within 24, 48, 72, 96 h was 0.600 6, 0.577 3, 0.567 5, 0.608 3 g/L, respectively. The results indicate that Chinese herbal medicines can't cure Cynoglossus semilaevis Gunther infeced by Scuticoci liatlda.

Since the outbreak of the COVID-19, All universities seriously implemented the instruction of the Ministry of Education, carried out the online-teaching earnestly. This thesis introduces the practice of online-teaching of College English course, compares and analyses the advantages and disadvantages of traditional teaching mode and online-teaching mode. It also explores the actualization of blended-teaching mode in the post-epidemic era.

Behavioral ecology is a discipline that studies the evolutionary basis of animal behavior due to ecological pressure. After studying the four problems that need to be solved and the direct causes, ontogeny, survival value and systematics of animal behavior, behavioral ecology emerges from ethology. Studying the causes and laws of these behaviors can help us better understand self-discipline and protect animals. Transcriptomics is a science that studies all transcripts of a specific cell, tissue or organ at a specific stage of growth and development or under a certain physiological condition. Different from the relatively stable genome, transcriptomics will change with the change of growth and development stage, physiological state and external environment. Therefore, we can study the mechanism of biological growth and development, stress physiology, disease resistance and immunity through transcriptome analysis. The behavioral research of Apostichopus japonicus is of great significance for improving the production management mode, improving the breeding mode, improving the harvesting efficiency, designing the harvesting facilities and investigating. The transcriptomics of Apostichopus japonicus is more like a key, which can better help us open the door of behavioral research, help us better understand the causes and regulatory mechanism of Apostichopus japonicus behavior, and make more efficient use of Apostichopus japonicus behavior for production and breeding. In this paper, the research status on different behaviors of Apostichopus japonicus, such as movement, feeding, clustering, stress, reproduction, competition and hibernation, was reviewed, and the transcriptomics related to the behavior of Apostichopus japonicus was summarized, so as to provide reference for the behavioral ecology and transcriptomics of Apostichopus japonicus in the future.

Goldfish industry in Tianjin, which is one of the three goldfish headstreams in China, with more than three hundred years of history is an important part of Tianjin aquarium industry. Through an extensive investigation into practitioners of goldfish industry, the study collected the industry data and historical materials to describe the development history and development status of goldfish industry in Tianjin. The results indicated a fact of scale declining of goldfish keeping, the decline or even lose of traditionally dominant varieties and the aging of practitioner in goldfish industry of Tianjin. Finally, the method of PEST was used to analyse the factors that affected the development of goldfish industry in Tianjin to find corresponding strategies. The study aimed to provide ideas for the healthy development of the goldfish industry in Tianjin and references for the formulation of the industrial policies.

In recent years, small and medium-sized scientific and technological enterprises in Tianjin have grown rapidly, and have become an important force to spur economic growth. Innovation is the soul of survival and development for small and medium-sized scientific and technological enterprises. At present, small and medium-sized scientific and technological enterprises in Tianjin have vigorous innovation will, good innovation environment and outstanding innovation achievements, but they still face the restrictions of fund, talents and policies. Furthermore, there are distinct differences of innovation abilities between the enterprises of different stages. In order to implement and consolidate the overwhelming development situation of small and medium-sized scientific and technological enterprises in Tianjin, we should innovate the personnel training mode and talent administration system, deepen the innovation of financial science and technology, establishthe precision and quasi support policies with echelon and difference, and improve the rate of implementation of the support policies.

Concrete is made up of four basic materials, which are cement, water, sand and stone. The factors influencing the concrete strength are water-cement ratio, cement mark, the quality of aggregate, curing period, the late maintenance and construction method, etc. Concrete has many advantages, such as strong strength and durability, low cost of local materials, fine plasticity of concrete mixture, and it can be modeled into any structure and size. Because of the similar expansion coefficient of concrete to rebar, its combination with rebar composes reinforced concrete structure, which is strong, durable, antiseismic and economical. In this article, the experiment about mixing proportion of concrete is carried out, and the reasonable ratio and technologies are obtained for improving the concrete strength.