猪圆环病毒2型吉林株的分离鉴定及全基因序列分析

Journal of Tianjin Agricultural University ›› 2016, Vol. 23 ›› Issue (4): 43-46.

• Researches and Scientific Notes • Previous Articles Next Articles

Isolation and Identification of Porcine Circovirus 2（PCV2）Isolated from Jilin Province and Its Complete Genome Sequence Analysis

WANG Dong-yuan^{1, 2}, WANG Yu-shun², GU Yu², JIN Tian-ming³, GAO Feng^{1,Corresponding Author}

1. College of Veterinary Medicine, Jilin University, Changchun 130062, China;
2. Beichen Breeding Industry Development Service Center, Tianjin 300400, China;
3. College of Animal Science and Veterinary, Tianjin Agricultural University, Tianjin 300384, China

Received:2016-07-18 Online:2016-12-20 Published:2019-10-14

Abstract

Abstract: An suspected porcine circovirus disease was confirmed by necropsy, histopathology observation, virus isolation and PCR detection, which was caused by porcine circovirus 2（PCV2） infection. A porcine circovirus strain（PCV2-JL/China/2015）was successfully isolated fromspleens and lymph nodesby passaging in PK-15 cells. A pair of primers based on PCV2 was designed according to the published sequences in GenBank. The length of complete genome of PCV2-JL/China/2015 was 1 767 bp by sequencing analysis. The results of the phylogenetic tree analysis indicated that there was a close genetic relationship between PCV2-JL/China/2015 and FJ594471-1C01 isolate from Changsha city.

Key words: porcine circovirus 2, isolation and identification, complete genome sequence

CLC Number:

S852.65

WANG Dong-yuan, WANG Yu-shun, GU Yu, JIN Tian-ming, GAO Feng. Isolation and Identification of Porcine Circovirus 2（PCV2）Isolated from Jilin Province and Its Complete Genome Sequence Analysis[J]. Journal of Tianjin Agricultural University, 2016, 23(4): 43-46.

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Isolation and Identification of Porcine Circovirus 2（PCV2）Isolated from Jilin Province and Its Complete Genome Sequence Analysis

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