An intelligent feeding system was proposed in this paper to improve the intellectualization of the farm, to save manpower and fullfull the precise feeding of livestock. The system was controlled by the single chip microcomputer to realize precise selection, dosing and weighing of the feeder. By pressing buttons in this system, the time of feeding and amount of food of each feeding time can be set and the livestock was fed automatically at the setting time. The water was supplied continuously and controlled by water saving control circuit to get flow control and water cost statistics. The temperature, humidity and lighting monitor and display in real time and other functions were included in this system. In addition, solar power was utilized by the system considering energy saving.

Chlorella is one of the earliest unicellular microalgae on earth, which is rich in protein, carbohydrate, minerals and vitamins and other nutritional substances based on the presence of its growth factor（CGF）. Recently, domestic and overseas scholars have paid close attention to CGF. CGF extraction method, physiological function and application progress of Chlorella were concluded and summarized in this paper, in order to provide theoretical basis and guidance for the development and application of CGF application from Chlorella.

Porosity, high cation exchange capacity（CEC）and low bulk density are main features of biochar, and it has a positive effect on improvement of soil properties and crop growth. The characteristics of biochar was analyzed, the influence of biochar on soil properties and growth of maize was discussed and biochar application in the future was given in the paper. The huge potential of biochar in the agricultural fields was also illustrated. Our principal objective in this paper was to provide relevant references for the research about the impacts of biochar on soil properties and growth of maize .

With the development of nano-medicine, a novel kind of self-assembled nanoparticle was fabricated through electrostatic crosslink method using negative-charged lecithin and positive-charged chitosan. This kind of self-assembled nano-carrier could be used to improve oral bioavailability of drugs with poor solubility. In this study, we utilized this nano-carrier to facilitate the drug delivery of berberine hydrochloride, whose water solubility is low to 1:200（g:mL）. At the same time, the method for entrapment efficiency（ EE）measurement was established. Afterwards, orthogonal screening was used to determine the optimal formulation of drug-loaded self-assembled nanoparticle, in which entrapment efficiency（ EE）was set as evaluation indicator. In the selected formulation, pH value of water phase was 8.0, volume ratio of ethanol phase/water phase was 8:92, and mass ratio of lecithin/chitosan was 20:1. Three batches self-assembled nanoparticle were prepared under the selected formulation, and evaluation results showed that the average particle size, Zeta potential and entrapment efficiency was（214.8±9.3）nm,（24.3±3.1）mV and（82.4±5.2）%, separately. Berberine hydrochloride loaded self-assembled nanoparticle remains high stability under 4 ℃ storage, which provided the basis for practical application.

Canine lymphosarcoma also known as malignant lymphoma is one of the most common tumors in canine tumors. A series of detailed clinical examinations for an eight-year-old dog with a spleen tumor was conducted, such as routine clinical examination, X-ray examination, hematology examination and pathological histology examination and then the splenic tumors were removed by exploratory laparotomy and pathological histology examination was carried out. Histopathological observation showed that lymphoid cells were patchy, round or nearly round, with a small amount of reticular collagen fibers in the stroma, few cytoplasm, hyperchromatic nuclei and more mitotic phenomena. There were hemorrhage and hemosiderin cells in the stroma and multiple necrotic foci in the center, thus diagnosing malignant lymphosarcoma of the spleen and providing basis for the treatment and prognosis of the disease.

In recent years, small and medium-sized scientific and technological enterprises in Tianjin have grown rapidly, and have become an important force to spur economic growth. Innovation is the soul of survival and development for small and medium-sized scientific and technological enterprises. At present, small and medium-sized scientific and technological enterprises in Tianjin have vigorous innovation will, good innovation environment and outstanding innovation achievements, but they still face the restrictions of fund, talents and policies. Furthermore, there are distinct differences of innovation abilities between the enterprises of different stages. In order to implement and consolidate the overwhelming development situation of small and medium-sized scientific and technological enterprises in Tianjin, we should innovate the personnel training mode and talent administration system, deepen the innovation of financial science and technology, establishthe precision and quasi support policies with echelon and difference, and improve the rate of implementation of the support policies.

Mesotrione is an important herbicide in maize field, and one of its main degradation pathways is microbial degradation. HF/6-31G（d）was used to perform the structural optimization and frequency analysis of mesotrione. The possible oxidation and reduction reactions in micro-organisms of the compound were studied. The entropy and Gibbs free energy of different redox processes were compared. The calculation results showed that mesotrione will give priority to produce pro6 with the catalytic effect of enzymes in microorganisms, which helps us to further understand the degradation behavior of mesotrione by microorganisms in environment.

Rural evitalization needs talent revitalization, and the agricultural management talents play an important role in the allocation of agricultural and rural resources. However, the agricultural management talents cultivated in colleges and universities are not necessarily willing to engage in agriculture-related work. This article takes the students of agricultural management school of Tianjin Agricultural University as the survey object, obtains data through questionnaire survey, uses regression model and description analysis, and empirically analyzes the willingness and influencing factors of agricultural management personnel engaged in agriculture-related work. Studies have shown that the nature of household registration, attitudes towards the majors studied, the degree of conformity of the profession with social needs（level of adaptation）, availability of value, policy attraction, and material security have a bearing on the willingness of agricultural management personnel to engage in agricultural work. Therefore, we must increase professional knowledge development and practice, strengthen policy guidance, give full play to the values of agricultural management professionals, and promote the realization of the strategy of rural rejuvenation.

To study the effects of different concentrations of enrofloxacin treatments on physiology and biochemistry of A. ocellaris, enrofloxacin soaking experiments（0, 5, 10 mg/L）were performed on three groups of A. ocellaris. The results showed that among the digestive enzymes activities, enrofloxacin had the most significant effect on trypsin and pepsin, followed by lipase and amylase. The order of enrofloxacin on the liver immune enzymes of the individuals was glutathione peroxidase（GSH）>alkaline phosphatase（AKP）, lysozyme（LZM）, glutathione S-transferase（GST）>superoxide dismutase（SOD）, catalase（CAT）, peroxidase（POD）. The influence of oxidation products is more significant in lipid peroxide（LPO ）than malondialdehyde（MDA）. Enrofloxacin had an effect on the activities of various enzymes and the content of oxidized products in A. ocellaris, and the effect of soaking treatment had significant difference（ P<0.05）. This result is of great significance for the disease treatment of the A. ocellaris.

Technology-based firms are the important strength for the pattern transform of economic growth in our country and the main force fortransformation of sci-tech achievements. When the enterprise has entered the maturity, it is the key time to transform or decay; its core rigidities, conservative trap in the management, and the entrepreneur’s vision and pattern will hinder the enterprise development. It is a reasonable route to transform the enterprise culture in timewith the base of entrepreneurship, driving by leadership alliance, and using the culture chain model concludingfeedback, reflection, action and internalization.

Bladder calculi and pyometra are common clinical cases of pets with high morbidity. Dog calculi is a stone in the urinary system due to various reasons. Dog stones include kidney, ureter, bladder and urethral stones, among which bladder and urethral calculi are the most common especially. A stone can cause a pet to urinate badly, no urine, abdominal pain, and severe urine leakage in the abdominal cavity, causing peritonitis. Pyometra is due to endometrial hyperplasia, the existence of a purulent material in the uterus accumulation of fluid. It is easy to increase the incidence rate and mortality rate of pyometra after uterus infection. However, there is only few cases when both bladder calculi and pyometra occur in one dog. This report followed up cases of 8-year-old pekingese and 7-year-old poodles suffering both from bladder calculi and pyometra. After clinical diagnosis, such as blood routine examination, biochemical examination, X-ray examination and b-mode ultrasound examination, they were finally diagnosed as the complication of bladder calculi and pyometra. Two cases had bladder calculi removed and uterus removed by surgery. Through drug treatment and postoperative care, spirit of the wound recovered with normal eating and micturition recovery after a week. The stitches were removed 10 days later and the wound healed well. After the clinical symptoms of the disease were analyzed, the systematic research report on the professional clinical examination, detailed treatment and nursing can provide clinical reference for the treatment of bladder calculi and uterus suppurative disease.

To determine the optimum pH conditions and provide basic information for intensive production of Chlorella vulgaris, the effects of different pH values on chlorophyll fluorescence, chlorophyll content and cell density during its culture process have been studied. The results show that when pH is 9 the maximum energy conversion rate（ Fv/ Fm）, the actual photochemical efficiency（ ΦPSⅡ）and the quantum efficiency（ Yield）decreased; meanwhile, the potential energy（ F_v/ F₀）, relative electronic conversion rate（ ETR）, chlorophyll content（ CHL）and the density of Chlorella increasing trend was minimum. When pH is 7, chlorophyll fluorescence are higher than the other groups with the values 1 613.05 µg/L, 1.13×10 ⁷ cells/mL respectively. The chlorophyll content and cell density of pH 9 treatment group were 883.82 µg/L and 6.77×10 ⁶cells/mL. The optimum growth pH of Chlorella is 7, and pH 9 can obviously inhibit the growth of Chlorella.

Escherichia coli with special serotype has different degrees of pathogenicity to human and animal intestines, among which enterotoxigenic Escherichia coli（ETEC）is more serious. Rectal swabs samples of 15 diarrhoea pigs in Tianjin area were collected and inoculated with MH, MacConkey Agar and eosin-methylene blue agar for bacterial culture and Gram staining microscopy.The colonies with different shape on the medium were tested for biochemical tests, 16S rRNA sequencing and BLAST analysis. The homology rate of 10 strains of Enterobacteriaceae and Escherichia coli was 99.6%. The specific primers of Escherichia coli enterotoxin ST1, ST2, LT1 and IR1 were synthesized. PCR results showed that 3 strains of Escherichia coli producing LT1 toxin, LT1 and ST1 toxin, and ST1 enterotoxin were isolated. The antimicrobial susceptibility test of isolated strains of enterotoxin LTI and ST1 showed that enterotoxigenic Escherichia coli was sensitive to neomycin, clindamycin, gentamicin, penicillin, ceftriaxone, amikacin, cefuroxime and florfenicol, which provided a basis for the monitoring and prevention of ETEC.

To affirm the optimized chemical constitution of submerged fermentation culture medium for Monascus exopolysaccharide（EPS）, the experiment was carried out with rice powder as the main substrate medium and by single factor, orthogonal and other experimental methods. The result shows that the best supplementary nitrogen source is soybean powder added to 10 mg/mL. The yield of EPS was inhibited by MnSO ₄, CaCl ₂, CuSO ₄, FeCl ₃, FeCl ₂, ZnSO ₄, and activated by KCl, KH ₂PO ₄, NaCl. In addition, all of these inorganic salts’ amount added are 0.15 mg/mL. Consequently, the fermentation mediums were optimized as follows: the powder of rice and the powder of soybean each account for 40 mg/mL and 10 mg/mL of the total amount. The dosages of KCl, KH ₂PO ₄ and NaCl were all 0.15 mg/mL. Under this condition, the yield of EPS reached 112.3 mg/mL on average, after culture age of 120 h. It is about twice the yield of liquid fermented polysaccharides based on rice flour alone.

In this study, minced beef bone（the mass ratio of bone to meat is 3∶7）was used as raw material. Traditional, spicy and fermented beef flavor were prepared by hot-pressing extraction and enzymatic hydrolysis followed by Maillard reaction with adding potassium sorbate in beef flavor as control group. The pH value, water activity and brightness of beef flavor were measured during storage at 4 ℃ and 20 ℃. The results showed that the pH values of BF with spices were different from those of other groups, and the changes of brightness and water activity were the same among the six groups. The shelf life of the beef flavor can reach 2.5 months and 40 d at 4 ℃ and 20 ℃, respectively. After modification, the product was stratified obviously.

Berberine hydrochloride is effective medicine for gastrointestinal bacterial infection treatment clinically. However, as for its poor lipophilicity, oral bioavailability of beriberine hydrochloride is relatively low. This study was designed to increase the lipophilicity of berberine hydrochloride using phospholipid complexation technology. In this study, associative efficiency（ AE） was set as screening parameter and optimal formulation of berberine hydrochloride phospholipid complex was obtained using solvent evaporation method based on single factor screening and orthogonal design. Under the selected formulation, AE of berberine hydrochloride phospholipid complex could reach 90% and storage stability remained in 6 months under 4 ℃, which provided the basis for practical application.

The eukaryotic expression plasmid pIRES2-AcGFP1-PLIN2 was constructed and its transfection status in 3T3-L1 preadipocytes was observed. The PLIN2 gene was cloned into pMD18-T vector and digested by EcoR I/ Sal I. The digested product was digested and then ligated into pIRES2-AcGFP1 vector and transformed into DH5α competent cells, and pIRES2-AcGFP1-PLIN2 positive recombinant plasmid was extracted and transfected into 3T3-L1 preadipocytes. The Transfection condition was observed by microscope. The PLIN2 gene was successfully cloned. At the same time, the eukaryotic expression plasmid pIRES2-AcGFP1-PLIN2 was successfully constructed and expressed in 3T3-L1 preadipocytes. The green fluorescence was observed under the microscope, which laid the foundation for further studying the regulation mechanism of PLIN2 gene on adipocytes.

Using the hydrostatic method, enrofloxacin was used to treat the juvenile larvae, the acid phosphatase（ACP）, alkaline phosphatase（AKP）and catalase（CAT）in the liver and hind kidney. Antioxidant enzyme activities such as peroxidase（POD） and superoxide dismutase（SOD）were studied. The results showed that enrofloxacin had significant effects on ACP, CAT, POD and SOD activities in the liver and hind kidney（P＜0.05）; And the activity was related to drug concentration and duration of action. This study provides a reference for enrofloxacin in the mechanism of action and disease prevention.

Fermentation biogenic gas is generally composed of several renewable energy gases with complex and changeable composition. A method for determination of hydrogen, nitrogen, methane and carbon dioxide simultaneously in fermentation biogenic gas was established by gas chromatography in this study. The chromatographic peaks were identified efficiently. The standard curves were well fitted in their linear ranges and the component analysis method was capable of very high precision and accuracy. This method was applied in the component analysis of the produced gas in two-stage hydrogen-methane fermentation process for treating food waste, and the way of main composition of fermentation gas change in batch experiment was achieved.

In order to ensure the quality of cultivated land after the reclamation of rural residential land, the northern coastal salinization area is taken as the research area, and the representative Tianjin Gegu Town of Jinnan District is selected to carry out the research of rural homestead reclamation mode. Based on four kinds of reclamation techniques, such as water and salt regulation, soil function reconstruction, biological selection and micro-regional soil improvement, three integrated reclamation modes were proposed. Soil salinity, organic matter, alkali-soluble nitrogen, available phosphorus and available potassium were selected as evaluation indexes. The fuzzy comprehensive evaluation method based on principal component analysis was used to evaluate the effect layering of different reclamation modes. The results showed that the soil nutrients in the layers before reclaimation were moderately low, and the soil nutrients in the near ground were worse than those in the lower layer. After one year of reclamation, the reclaimed integrated model 1 improved the organic matter, alkaline nitrogen and available potassium; the mode 2 improved the evaluation indexes in the soil; the mode 3 improved the salt content in the soil. The three integrated reclamation modes increased the content of organic matter, alkali nitrogen and available potassium in various layers of soil, reduced the salt content in the soil, and imporved the soil nutrients to a high level. The study can provide technical support and theoretical basis for the reclamation of homesteads in the northern coastal salinization area.

The healthy development of the green food industry in Tianjin is significant for promoting the supply-side reform in the agricultural sector, improving the quality of agricultural products, and satisfying the demand of the urban and rural residents for the agricultural products with high quality and safety. At the same time, it is a strategic action to practice the concept of green ecological development and promote the transformation of green agriculture. This paper summarized the achievements and problems in the development of green food industry in Tianjin. Meanwhile, it also analyzed the advantages and opportunities of the development of green food industry in depth, based on twhich, it put forward some countermeasures to promote the healthy development of green food industry in Tianjin.

The appearance of red parrot fish is an important factor of grading, and the grading results determine its market value. At present, the classification of blood parrot fish mainly relies on artificial, with strong subjectivity and low efficiency. This paper introduced the use of machine vision technology to evaluate the parrot fish appearance by detailed elaboration under the invariable condition of aquaculture water, through Matlab2017a camera image acquisition software control, and through the binarization, threshold, de-noising, finding the boundary, processing the collected image, finally introducing the use of metric function to judge whether the fish boundary of closed curve is tend to be rounded so as to complete blood parrot fish shape evaluation. The experimental results showed that this method can automatically complete the classification of blood parrot fish and solve the problem of low efficiency and large error existing in traditional methods.

A 4-year-old female cat was examined by blood biochemistry. The results showed that ALT was 436 u/L, ALKP was 249 u/L, TBIL was 88 μmol/L, which was higher than the normal value. Combined with the digestive tract symptoms such as fever, depression, loss of appetite, vomiting and severe jaundice, the cat was diagnosed as liver failure. According to the clinical symptoms and the results of hematological tests, the cat was treated with a comprehensive treatment for more than a month. The results showed that the values of ALT, ALKP and TBIL all reached normal values, and the cat’s diet and defecation were normal, indicating that the cat had basically recovered. The follow-up report of this case can provide reference for clinical diagnosis and treatment of pets.

In order to clarify the associated pathogens of companion animal suppurative diseases, uterine secretions from a patient pyometra dog at animal hospital affiliated to the college were collected sterilely for pathogen isolation and identification. The isolate was identified as Citrobacter freundii by bacterial isolation and culture, colony morphology observation and Gram’s staining observation, biochemical test and 16S rRNA gene analysis. Antibiotic susceptibility results showed that the isolated bacterium is resistant to Maddie and ampicillin, moderately sensitive to aztreonam, cefazolin, and cefoxitin, highly sensitive to cefoperazone, ceftriaxone, cefepime and other antibiotics, providing reference for prevention and related diseases control of companion animals.

Flavonoid ingredients are abundant in effective extracts from Radix angelicae sinensis with bioactivities of anti-aging and scavenging free radicals through topical administration. Aiming at these characteristics, ointment was prepared for extracts from Radix angelicae sinensis with water-in-oil（W/O）type. At the same time, orthogonal screening was used in formulation selection in order to obtain stable ointment formulation with preferred skin-feeling. In this investigation, flavonoid ingredients were acquired through ultrasonic extraction firstly. Afterwards, comprehensive scoring of homogeneity, extensibility, centrifugal stability and cold/heat resistance was set as the evaluation indicator to obtain the optimal proportion of oil phase, emulsifier and coemulsifier. In this ointment formulation, Span-60 and Tween-80 were used as emulsifier and coemulsifier respectively, and lanolin/vaselin/stearic were used as muti-compositional oil phase with quality ratio of 1∶3∶4. After prepared under the optimal formulation, in which the mass percentage of oil phase, Span-60 and Tween-80 were 35.0%, 4.0% and 4.5% respectively, stable ointment was acquired in oil-in-water type. This study provides the basis for further application of Radix angelicae sinensis extracts ointment.

Listeria monocytogenes is an important food-borne pathogen which may contaminate many types of food and impair food safety. Research methods of predictive food microbiology can be used for building growth kinetic models, which may assess microbial risk and predict food shelf life. This article introduced growth characteristics of L. monocytogenes. The classification of primary, secondary and tertiary models of predictive food microbiology were briefly reviewed. Applications of L. monocytogenes growth kinetic models in culture medium and different foods were summarized.

In order to study the pathogenicity of early infection of Eimeria falciformis in mice, E. falciformis was invading by oral infection in the treatment group, while the control group was established with non-coccidia infection. Then the clinical symptoms, autopsy lesions, organ index, organ pathological histology and immunohistochemical characterization of cecum were observed for 36 hours to learn about the pathogenicity of early infection of E. falciformis in mice. The results showed that in the early stage of infection, E. falciformis maily damaged mucosal tissues of intestinal tract which caused degeneration, necrosis and shedding of epithelial and glandular epithelial cells, edema in strata submucosum, inflammatory cells infiltrating the mucosa but no visible pathological damage to other organs. According to immunohis chemical observation, the infections of mucosa and submucosa in the early stage were also observed. Hence, E. falciformis will be a candidate vaccine vector, which did not damage all the organs except cecum.

In order to achieve early detection and early intervention of college students' psychological problems, this paper puts forward the construction of mental health monitoring system, which is composed of early warning information collection, early warning monitoring and intervention treatment. This system includes quantitative and qualitative monitoring. Quantitative monitoring includes psychological scale, economic, academic, physical health and interpersonal relationship status; qualitative monitoring includes speech and deportment, emotion, learning attitude, communication style, discipline and emergency. This system has a guiding significance to maintain the safety and stability of college students and to improve the level of mental health of college students.

In the new media environment, mobile phone culture has an important impact on college students. The use of mobile phones has advantages and disadvantages in the process of teaching and learning. How to use the mobile phone reasonably and efficiently to reform the educational model, especially the reform of the ideological and political education mode, is an important task that needs to be solved urgently in the current higher education. The paper probed into the motivation of college students to use mobile phones and the phenomenon of mobile phone dependence by using the investigation method. The negative and positive effects of mobile phones on college students' ideological and political education were analyzed. The innovation model of Ideological and political education with mobile phone such as "online" and "offline" education as well as mobile internet communication platform was put forward.

Aiming at the problems that some henhouse environments are bad so that hens are easily infected by some bacteria and viruses, environmental monitoring system in henhouse is developed based on ZigBee 2007/PRO and android application. Three main parameters, such as temperature and humidity, illumination intensity and ammonia concentration are collected; and then ventilators and lights are controlled. The system employs CC2530 of TI Company as coordinators and terminals. Sensors and control devices are connected with terminals, which are properly distributed in henhouse, and then ZigBee2007/PRO is used to build ZigBee star network. Environmental parameters are collected from terminals and transferred to a coordinator and mobile phone, and then the coordinator handles the environment parameter data and sends the instruction to the control terminal node to form the whole monitoring system.

In order to study the effect of transport on dogs, Labrador retrievers were chosen and blood was collected before transport andafter 2-hour transport. Then serum cortisol, adrenocorticotropic hormone and biochemical indices were determined. The results showed: the concentration of cortisol and the activity of glutamic oxalacetic transaminase (AST) were significantly increased after transport ( P<0.05). The activity of Creatine kinase (CK) was significantly increased ( P<0.01). Adrenocorticotropic hormone (ACTH), alanine transaminase (ALT), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), glucose (GLU) had no significant difference ( P>0.05). In conclusion, 2-hour transport stress can induce the change of serum cortisol, AST and CK of Labrador retriever.

The method for rapid detection of oxytetracycline residues in milk was established by combining mid-infrared （MIR） spectroscopy with partial least squares discriminant analysis（PLS-DA）. Firstly, 62 pure milk samples and 62 milk samples adulterated with different concentrations of oxytetracycline（0.02-30.00 mg/L）were prepared respectively. The MIR spectra of all samples were collected in the range of 400-4 000 cm ^-1. After the pretreatment of original spectral data, the PLS-DA models were established in the full band and sub band ranges, respectively. The results show that different wave-number ranges had a great influence on the prediction model of milk adulterated with oxytetracycline. The classification accuracies of the constructed PLS-DA models were 100% for calibration set in the full band and sub band ranges, and the correlation coefficients of models were between 0.995 and 0.997 for prediction set, which shows that the models had a good fitting effect. The classification accuracies of constructed models in the full band and in the range of 2 000-4 000 cm ^-1 were 90.0% for prediction set.

In order to know the incidence of skin diseases of pet dogs in Tianjin, an epidemiological survey was conducted on 166 cases of dogs with skin diseases admitted to a large pet hospital in Tianjin from March to June 2018, and statistical analysis was conducted from 5 aspects including breed, age, gender, pathogen and sterilization of the dogs. The results showed that there were 18 breeds of dogs, among which the incidence was 19.28% for poodles, 15.06% for Chinese pastoral dogs and 13.25% for bulldogs. The incidence of juvenile dogs under the age of 2 years was the majority, accounting for 56.02%; 2-9 years old adult dogs and middle-aged and elderly dogs were less at 22.89% and 10.24%, respectively; The incidence of elderly dogs over 10 years old was relatively high at 10.84%; Males accounted for 62.65%, more susceptible than females. Most of the cases were infectious skin diseases, mainly fungal infections, mostly mixed infections, and few other non-infectious skin diseases. Neutered dogs accounted for only 7.22 percent of the total. The results showed that the skin diseases of dogs in Tianjin from March to June were mainly fungal, mixed infections were the majority, and puppy infections accounted for a large proportion. This provides the basis for scientific prevention and treatment of skin diseases in pet clinic.

In order to provide the basic information for control of anthura bacterial blight in Tianjin, inhibition and control effects of fungicides such as ethylicin on Xanthomonas axonopodis pv. dieffenbachiae causing anthura bacterial blight were studied. Virulence of ten fungicides against X. axonopodis pv. dieffenbachiae were tested with oxford cup method. The control effects of five fungicides on bacterial blight were tested by spray methods in this study. The results showed that 80% ethylicin EC showed the most prominent inhibit effect, its EC ₅₀ value was 1.511 4 mg/L. Secondly, 70% Carmazine WP, 6% primomycin WP, 90% new mycin WP and 3% mesophyllin WP showed obvious inhibit effect on X. axonopodis pv. dieffenbachiae, and their EC ₅₀ value were lower, respectively, 18.348 9, 60.232 1, 78.666 6 mg/L and 84.447 2 mg/L. The EC ₅₀ values of 46% Kocide WP, 20% thiediazole-copper SC and 30% copper succinate WP were all over 100 mg/L. The inhibit effects of 20% bismerthiazol WP and 72% agricultural streptomycin WP were the worst. Five fungicides were selected and tested for the greenhouse effects of anthura bacterial blight. The results showed: Ethylicin had the highest control effect, up to 76.1%, and agricultural streptomycin had the lowest control effect, only 27.1%. The control effects were as follows: ethylicin>primomycin>new mycin>copper succinate>agricultural streptomycin. Ethylicin can control anthura bacterial blight efficiently and can be applied on the disease management. However, agricultural streptomycin was not advised to usebecause of its poor control effect.

The ideological and political theory course in colleges and universities must be guided by the speech of the general secretary of Xi Jinping. The spirit of the speech by the general secretary is the focus of this article. The new situation of the current ideological and political course construction are reexamined. The requirements and orientation of ideological and political education in the new era must be clearly defined by the party and the state. The ideological and political course should keep up with the times and follow the call of the times constantly repeat the old topic and make it new to fulfill our mission, so that we can constantly improve our ideological and political education.

In order to understand the anatomical differences of floral organ among different blueberry’s cultivars,the anatomical structure of blueberry in petals, filaments, anthers and other flower organs were scanned and observed by electron microscopy with four blueberry cultivars, ‘Berkeley’, ‘Brigitta’, ‘Lexie’ and ‘Bluecrop’. The results showed that the cell sizes of inside petals of different blueberries’ cultivars were different with ‘Bluecrop’（0.003 95 mm ²）largest one and ‘Brigitta’（0.002 24 mm ²）the smallest one. The epidermal hair in ‘Lexie’ was the densest; that in ‘Bluecrop’ was the sparsest. The tubular structure on the top of mature anther of ‘Berkeley’ was a little crossed, and while it was clearly separated in Brigitte. The pollen grains were tetrad composite pollen with ditch and carving.The stigma morphology of different blueberries’ cultivars was different, relatively flat in ‘Berkeley’ and more gullies in Burguita. The types and numbers of ovules in different blueberries’ cultivars were different. The ovule of ‘Bluecrop’ was inverted ovules, but the others were straight ovules. ‘Lexie’ had the most ovules in all cultivars（30）, ‘Brigitta’ had the least（10）. Therefore, there were differences in flower structures of different blueberries’ cultivars, which could be used as a classification of varieties.

The technology of remote sensing could quickly and nondestructively acquire a wide range of land surface information, and could be widely used in crop area monitoring, crop growth and yield estimation, and crop disasters monitoring etc.The development of remote sensing technology makes the agricultural monitoring more refined and real-time.The enhancement of remote sensing image quality and timeliness has greatly promoted the precision of agricultural monitoring.

In this paper, with the stolon tip of ‘Xiangye’ strawberry used as test material, the key technology of rapid propagation of explants such as sterilization, induction and differentiation culture, subculture and rooting culture were studied with tissue culture method, and the rapid propagation technology of strawberry cultivar ‘Xiangye’ stem tip tissue culture was established. The results showed that using 10% sodium hypochlorite to disinfect the explants for 5 minutes, the disinfection effect was the best and the survival rate was the highest, reaching 64.44%. The best medium for strawberry explant induction and differentiation was MS+Thidiazuron（TDZ）1.0 mg/L+Naphthalene acetic acid（NAA）0.1 mg/L, and the induction and differentiation rate was 67.78% at the stage of explant induction and differentiation. The best medium formula for cluster bud multiplication of strawberry was MS+TDZ（0.75 mg/L）+NAA（0.2 mg/L）, and the value-added coefficient was 4.11 at the stage of cluster bud subculture and multiplication. The optimum medium for rooting of strawberry cluster buds was MS+NAA 0.1 mg/L and the rooting rate was 98.33% in the rooting stage.

Irrigation water price is a key link in relation to agricultural reform and development. Reasonable agricultural irrigation water price can arouse the enthusiasm of water saving to realize the sustainable development of agriculture. The composition and influencing factors of the agricultural irrigation water price were analyzed in the paper. The formula of the total cost water price and the operating cost of the pumping irrigation district were derived, and the influence of the different hydrological years on the income of the irrigation project was probed, taking the water price reform in Jiashan County of Zhejiang Province as an example. The results show that the total cost water price is 0.441 yuan/m ³, and the operating cost water price of the project is 0.116 yuan/m ³ in pumping irrigation project. In the average water year（50% of the precipitation guarantee rate）, the annual water fee income is equal to the annual project cost; in the dry year（75% of the precipitation guarantee rate）, the annual water fee income is 1.33 times of annual project cost; in the extraordinary dry year（90% of the precipitation guarantee rate）, the annual water fee income is 1.45 times of annual project cost. Considering the frequency of different year types, such as wet year, normal water year, dry year, the benefits of water pumping irrigation project can satisfy its own operation and maintenance.

In order to study the influence of haze on the photosynthetic characteristics of evergreen in winter in North China, the net photosynthetic rate (Pn), transpiration rate, stonmatal conductance of leaves were analyzed by CL-340 and difference between species was compared with 4 representative evergreen（ Euonymus japonicus, Agave sisialana, Ligustrum lucidum, Buxus sinica var.parvifolia）in Tianjin. The results showed that the photosynthetic characteristic of all plants decreased significantly,and the net photosynthetic rate, transpiration rate, stonmatal conductance of 4 species were negatively correlated with PM2.5. The correlation coefficient of the PM2.5 content in the atmosphere and the net photosynthetic rate of the 4 species, Euonymus japonicus, Ligustrum lucidum, Agave sisialana, Buxus sinica var .parvifolia were respectively -0.87、-0.78、-0.88、-0.84,which showed a strong correlation; the correlation coefficient with transpiration rate of leaves were -0.82、-0.80、-0.72、-0.67 successively, meaning stronger correlation in Euonymus japonicus and Ligustrum lucidum, which were above 0.80; the correlation coefficient with stonmatal conductance were -0.737、-0.895、-0.794、-0.783 successively,which had a strong correlation with Agave sisialana. Meanwhile,the net photosynthetic rate of 4 species were siginificantly different under influence of haze. It shows： Agave sisialana has strong resistance to haze.