Journal of Tianjin Agricultural University ›› 2021, Vol. 28 ›› Issue (4): 27-30.doi: 10.19640/j.cnki.jtau.2021.04.007

• Researches and Scientific Notes • Previous Articles     Next Articles

Comparison of three corrosion methods for cast specimens of tracheobronchial duct in rabbits

Lü Jiaqi, Zhuge ZengyuCorresponding Author, Yao Yuxin, Shen Jusong, Zhen Zhen   

  1. College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin 300392, China
  • Received:2020-01-15 Online:2021-12-31 Published:2022-01-05

Abstract: To find out an effective and simple method to make the casting specimen of rabbit’s pulmonary trachea and bronchial tree, so as to provide a real and rigorous anatomical basis for veterinary related teaching, scientific research and clinical practice, three corrosion methods were used in this research. Self-solidifying denture powder and denture water were used to prepare fillers in proportion, and then perfusion from the throat into the lungs of rabbits. After the filler solidified, Sodium hydroxide solution, sulfuric acid solution and hydrochloric acid solution were used to corrode the lung tissues. The results showed that all of the three corrosion methods could get good cast specimen. The specimen corroded by sodium hydroxide solution only needed to be immersed in the same solution again; The specimens corroded by sulfuric acid solution were black and needed to be bleached again; The specimens corroded by hydrochloric acid solution needed to be immersed in alkaline solution to remove the residual lipids. After filling the lung with self-coagulating dental powder and dental bracket water at the ratio of 1.0∶1.5, 50% sodium hydroxide solution were used to corrode the lung tissue. The intact, moderately elastic, clean and smooth respiratory cast specimens could be obtained, and bronchial trees could be clearly seen. Sodium hydroxide solution did not need to be equipped with other solutions, and the substance was not volatile. It is the most convenient and safe method among the three corrosion methods.

Key words: rabbit, tracheal bronchus, specimen, casting mold, corrosion method

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