microRNA-139对小鼠失神经肌肉萎缩中肌纤维的影响

Journal of Tianjin Agricultural University ›› 2016, Vol. 23 ›› Issue (2): 6-10.

• Researches and Scientific Notes • Previous Articles Next Articles

Effects of MicroRNA-139 on Myofiber of Denevated Muscle Atrophy in Mouse

WANG Yi-min^{1, 2}, ZHANG Wan¹, ZHANG Wei-ran¹, DAI Yang¹, LIU Xin-feng¹, LI Xin¹, GUO Hong¹, DING Xiang-bin^{1Corresponding Author}

1. College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin 300384, China;
2. College of Veterinary Medicine and Key Laboratory of Animal Biotechnology, Ministry of Agriculture, Northwest A & F University, Yangling 712100, Shaanxi Province, China;

Received:2016-01-13 Online:2016-06-20 Published:2019-10-14

Abstract

Abstract: To investigate the effects of miR-139 on myofibers of denervated muscle atrophy in mouse, anamyotrophia mouse model of sciatic nerve resection was established. 10 nmol miR-139 inhibitors were injected into the denervated gastrocnemius. 14 days after injection, the expression of miR-139 was detected by qRT-PCR, the wet weight and the cross section diameter of the muscle fibers were compared, and the expression of myosin heavy chain（MHC）was examined by RT-PCR and Western blot analysis between the atrophying skeletal muscle and control. The results showed that the cross section diameter of the muscle fibers in treated group was significantly higher than that of control group. The results suggested that miR-139 may play an important role in muscle growth and differentiation in vivo.

Key words: mouse, denervation, amyotrophy, microRNA-139

CLC Number:

S852

WANG Yi-min, ZHANG Wan, ZHANG Wei-ran, DAI Yang, LIU Xin-feng, LI Xin, GUO Hong, DING Xiang-bin. Effects of MicroRNA-139 on Myofiber of Denevated Muscle Atrophy in Mouse[J]. Journal of Tianjin Agricultural University, 2016, 23(2): 6-10.

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: http://xuebao.tjau.edu.cn/EN/

http://xuebao.tjau.edu.cn/EN/Y2016/V23/I2/6

References

[1] Menotti M.The denervated muscle: facts and hypotheses. A historical review[J]. Eur J ApplPhysiol,2006,98:1-21.
[2] Borisov A B,Dedkov E I,Carlson B M.Differentiation of activated satellite cells in denervated muscle following single fusions in situ and in cell culture[J]. Histochem Cell Biol,2005,124(1):13-23.
[3] de Castro R A,Andreo J C,de Mattos Rodrigues S P. Myonuclei and satellite cells in denervated rat muscles:An electron microscopy study[J]. Microsurgery,2006,26(5):396-398.
[4] Bartel D P.MicroRNAs:Genomics,Biogenesis,Mechanism,and Function[J]. Cell,2004,116:281-297.
[5] Meister G,Tuschl T.Mechanisms of gene silencing by double-stranded RNA[J]. Nature,2004,431:343-349.
[6] Nakasa T,Ishikawa M,Shi M,et al.Acceleration of muscle regeneration by local injection of muscle-specific microRNAs in rat skeletal muscle injury model[J]. Journal of cellular and molecular medicine,2010,14(10):2495-2505.
[7] Ohira Y.Effects of denervation and deafferentation on mass and enzyme activity in rat skeletal muscles[J]. Jap J Physiol,1999,39(1):21-31.
[8] Czerwinska A M,Streminska W,Ciemerych M A,et al.Mouse gastrocnemius muscle regeneration after mechanical or cardiotoxin injury[J]. Folia Histochem Cytobiol,2012,50(1):144-153.
[9] Sakamoto K,Nosaka K,Shimegi S,et al.Creatine kinase release from regenerated muscles after eccentric contractions in rats[J]. Eur J Appl Physiol Occup Physiol,1996,73(6):516-520.
[10] Velleca M A,Wallace M C,Merlie J P.Anovelsynapse- associated noncoding RNA[J]. Mol Cell Biol,1994,14:7095-7104.
[11] 赵文勇,王涛,王军平,等. 肌肉特异性microRNAs在失神经肌肉萎缩中表达变化的研究[J]. 第三军医大学学报,2008,30(21):2034-2036.
[12] 王涛. 微小RNAmiR-1和miR-206促进成肌分化的作用及其机制研究[D]. 重庆:第三军医大学,2008.
[13] McCarthy J J,Esser K A,Peterson C A,et al. Evidence of MyomiR network regulation of β-myosin heavy chain gene expression during skeletal muscle atrophy[J]. Physiological genomics,2009,39(3):219-226.
[14] van Rooij E,Quiat D,Johnson B A,et al. A family of microRNAs encoded by myosin genes governs myosin expressio
n and muscle performance[J]. Developmental cell,2009,17(5):662-673.

Effects of MicroRNA-139 on Myofiber of Denevated Muscle Atrophy in Mouse

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 4

Recommended Articles

Metrics

Comments

[1]	ZHANG Hui-na, ZHANG Wei-ran, WANG Yi-min, LIU Xin-feng, GUO Hong, DING Xiang-bin. Effect of MicroRNA-143 on Myofiber of Denevated Muscle Atrophy in Mouse [J]. Journal of Tianjin Agricultural University, 2017, 24(2): 39-43.
[2]	DUAN Xian-ping, QIN Shun-yi. Establishment of Kidney Pathological Model Injured by Gentamycin in Mice [J]. Journal of Tianjin Agricultural University, 2014, 21(4): 33-34.
[3]	DAI Yang, WANG Yi-min, LIU Xin-feng, FAN Han-lu, LI Ji-xia, GUO Hong, DING Xiang-bin. Isolated Culture and Identification of Mouse Skeletal Muscle Satellite Cells [J]. Journal of Tianjin Agricultural University, 2014, 21(1): 1-4.
[4]	ZHAO Cui-li, GAO Peng-yun, MA Ji-fei, BIAN Yan. Study on Acute Toxicity of Agaricus Blazei Polysaccharide Oral Liquid in Mice [J]. Journal of Tianjin Agricultural University, 2013, 20(2): 38-39.