当归多糖除蛋白质前后抗氧化活性比较

Journal of Tianjin Agricultural University ›› 2017, Vol. 24 ›› Issue (3): 46-49.

• Researches and Scientific Notes • Previous Articles Next Articles

Compare of Antioxidant Activity of Angelica sinensis Polysaccharide before and after Protein Removing

LIU Bo, WANG Qing-kui, XING Ke-zhi^{Corresponding Author}, CHEN Cheng-xun

Tianjin Key Laboratory of Aqua-Ecology and Aquaculture, College of Fisheries, Tianjin Agricultural University, Tianjin 300384, China

Received:2016-12-05 Online:2017-09-30 Published:2019-10-15

Abstract

Abstract: ：This paper compared the antioxidant activity of Angelica sinensis polysaccharide（ASP）before and after protein removing. Crude A.sinensis polysaccharide（CASP）was extracted with hot water and precipitated with ethanol. The ASP was obtained by removing protein from CASP by Sevag method. Ferricyanide reduction method, fenton method, DPPH method, and adjacent benzene three phenol autoxidation method were adopted to assay the reduction ability and free radical-scavenging activity in vitro. The results showed that CASP and ASP exhibited significant reduction ability and free radical-scavenging activity（P<0.05）. Within the same concentration, the reduction ability and free radical-scavenging activity of CASP were higher than those of ASP（P<0.05）. However, the reduction ability and free radical-scavenging activity of CASP and ASP were feebler than those of ascorbic acid（P<0.05）. These results indicate that CASP and ASP exhibited antioxidant ability, the antioxidant ability of CASP was stronger than that of ASP.

Key words: Angelica sinensis, polysaccharide, reduction ability, free radical-scavenging activity

CLC Number:

R284.2

LIU Bo, WANG Qing-kui, XING Ke-zhi, CHEN Cheng-xun. Compare of Antioxidant Activity of Angelica sinensis Polysaccharide before and after Protein Removing[J]. Journal of Tianjin Agricultural University, 2017, 24(3): 46-49.

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: http://xuebao.tjau.edu.cn/EN/

http://xuebao.tjau.edu.cn/EN/Y2017/V24/I3/46

References

[1] 顾有方,陈会良,刘德义,等. 自由基的生理和病理作用[J]. 动物医学进展,2005,26(1):94-97.
[2] 王珺. 乙氧基喹啉、氧化鱼油和烟酸铬对大黄鱼与鲈鱼生长性能的影响及其(或代谢物)在鱼体组织中残留的研究[D]. 青岛:中国海洋大学,2010.
[3] 李尔春,丁红军,金晓辉. 天然植物多糖的结构及活性研究进展[J]. 食品与药品,2007,9(4):51-54.
[4] 冯学花,梁肖蕾. 当归化学成分与药理作用的研究进展[J]. 广州化工,2012,40(22):16-18.
[5] 田苏阳,郝长春,孙润广,等. 超声波提取的当归多糖化学修饰及其抗氧化活性研究[J]. 植物科学学报,2015,33(4):545-553.
[6] 安静. 当归提取物中不同组分配伍的协同抗炎、抗氧化作用研究[D]. 北京:北京中医药大学,2014.
[7] 张庆增. 复合酶法提取当归多糖及其生物活性研究[D]. 长春:吉林大学,2006.
[8] 李贵荣,吕昌银,杨胜圆. 当归多糖清除活性氧自由基作用的研究[J]. 南华大学学报(理工版),2002,16(3):18-20.
[9] 张清勇,于宏,王庆奎,等. 当归多糖的提取、分离及纯化[J]. 河北渔业,2016(3):1-3.
[10] 王庆奎,邢克智,赵海运,等. 当归多糖除蛋白质的方法与条件优化[J]. 时珍国医国药,2011(2):525-526.
[11] 盖玉红. 灵芝多糖抗氧化活性研究[D]. 长春:吉林农业大学,2007.
[12] 陈玉琴,南海娟,刘坤峰. 蕨菜多糖提取及抗氧化特性[J]. 北方园艺,2014(14):128-132.
[13] 葛霞,陈婷婷,蔡教英,等. 青钱柳多糖抗氧化活性的研究[J]. 中国食品学报,2011,11(5):59–64.
[14] 戴宏杰. 虎斑乌贼肌肉糖蛋白分离纯化、结构分析及其抗氧化活性初步研究[D]. 杭州:浙江工商大学,2015.
[15] 刘松,董晓芳,佟建明. 多糖提取和抗氧化活性评价方法的研究现状和进展[J]. 动物营养学报,2016,28(11):3391-3399.
[16] 赵焕焕,贾陆,裴迎新. 黄秋葵粗多糖体外抗氧化活性测定[J]. 郑州大学学报(医学版),2012,47(1):40-43.
[17] 梁静,刘晓宇,邓碧云,等. 红枣粗多糖的提取及抗氧化活性评价[J]. 食品科技,2012,37(1):177-181.
[18] 张越锋,李福燕,吴瑛. 红枣多糖及红枣硒多糖抗氧化活性的比较研究[J]. 食品研究与开发,2015,36(3):4-9.
[19] 王丽霞,刘安军,舒媛,等. 山药蛋白多糖体外抗氧化作用的研究[J]. 现代生物医学进展,2008,8(2):242-245.
[20] 梁引库. 黄精多糖的脱色、脱蛋白及体外抗氧化活性研究[J]. 江苏农业科学,2013,41(5):240-242.
[21] 熊莉,王承明. 花生粕多糖去蛋白方法的研究[J]. 食品科技,2010,35(9):219-222.
[22] 于翠莲. 核桃青皮粗多糖的提取、理化性质及其抗氧化活性的研究[D]. 保定:河北农业大学,2012.
[23] 边静静,付娟,赵桦. 娑罗子多糖的提取、含量测定及生物学活性研究[J]. 食品工业科技,2010,31(10):72-74,77.
[24] 郭永月. 黑牛肝菌多糖对小鼠酒精性肝损伤的保护作用研究[D]. 南京:南京师范大学,2013.
[25] 杨晓杰,董亚楠,李娜,等. 桔梗多糖的抑菌性和抗氧化性研究[J]. 食品研究与开发,2015,36(21):12-14.
[26] 颜军,徐光域,郭晓强,等. 银耳粗多糖的纯化及抗氧化活性研究[J]. 食品科学,2005,26(9):151-154.

Compare of Antioxidant Activity of Angelica sinensis Polysaccharide before and after Protein Removing

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	Li Xiang, Zhang Pingping, Zhang Huan, Zhang Yangyang, Lan Zhixiang. Optimization of extraction process and preliminary structural analysis of polysaccharide from Xiuzhen mushroom by response surface methodology [J]. Journal of Tianjin Agricultural University, 2024, 31(2): 38-44.
[2]	Zhang Yang, Lu Hegang, You Pengzhi, Li Xiaoyan, Huang Haidong. Screening, identification of strain ZYRH-19 and characteristics of its product welan gum [J]. Journal of Tianjin Agricultural University, 2024, 31(1): 36-40.
[3]	Yang Zongfen, Wang Min, Wang Zhenli, Wang Haifeng, Wang Junbin. Study on antioxidant and antibacterial activity of polysaccharide extract in Mesembryanthemum crystallinum [J]. Journal of Tianjin Agricultural University, 2023, 30(1): 1-5.
[4]	Wei Yating, Zhang Jin, Pan Jing, Li Zhaojie, Yang Hongpeng. Study on extraction technology of soybean meal polysaccharide by compound enzyme [J]. Journal of Tianjin Agricultural University, 2022, 29(2): 27-31.
[5]	Xu Xianmei, Cai Fenglong, Wu Fengming, Li Liuan, Zhang Jianbin. Study on the preparation of pullulan polysaccharide nanoparticles by dialysis [J]. Journal of Tianjin Agricultural University, 2021, 28(4): 23-26.
[6]	Guo Yi, Li Yiteng, Wu Haiqing, Wang Bujiang, Xiao Ping, Liu Jinfu. Effect of bitter melon saponins with pumpkin polysaccharides or buckwheat flavones on glucose and lipid metabolism in hyperlipidemia rats [J]. Journal of Tianjin Agricultural University, 2021, 28(2): 51-55.
[7]	Guo Yi, Yang Xuan, Xiao Ping. Study on extraction process of quinoa polysaccharide by response surface methodogy [J]. Journal of Tianjin Agricultural University, 2020, 27(4): 43-48.
[8]	FENG Zi-yao, ZHANG Ping-ping, ZHANG Hong-ying, LIN Xiang-jin, CHUAI Dong-hua, ZHANG Sheng-kang. Study on processing technology optimization of compound Pleurotus-eryngii tablets [J]. Journal of Tianjin Agricultural University, 2020, 27(3): 62-65.
[9]	GUO Yi, YU Jing-jing, XIAO Ping. Study on the technology of extracting polysaccharides from Radix Puerariae by Ultra-high pressure method [J]. Journal of Tianjin Agricultural University, 2020, 27(3): 66-69.
[10]	LI Yue-chan, ZHANG Zi-yi, WEI Xue-yuan, CHANG Li, YANG Wei. Optimization of conditions for the production of exopolysaccharides by Monascus and preliminary detection of efficacy activity [J]. Journal of Tianjin Agricultural University, 2020, 27(2): 79-83.
[11]	TIAN Shu-yao, YANG Yu, HU Yue, WU Zhi-wei, ZHANG Zi-hui, YUAN Yan-yang, LI Fu-gui. Comparative study of effect on immune efficiency toNewcastle disease with fifteen intensifiers [J]. Journal of Tianjin Agricultural University, 2020, 27(1): 50-52.
[12]	SUN Kang-di, LIU Qing-yun, FANG Yong-qing, CHENG Zi-kai, LI Fu-gui. Effects of astragalus polysaccharides, ganoderma polysaccharides and transfer factor on immune-efficiency to canine distemper [J]. Journal of Tianjin Agricultural University, 2020, 27(1): 58-60.
[13]	CHENG Bin, LI Xiao-yan, PAN Yi-chen, FENG Xue, HUANG Hai-dong. Screening, identification of sanxan-degrading microorganisms and antioxidant activities of degradation product [J]. Journal of Tianjin Agricultural University, 2019, 26(4): 43-48.
[14]	ZHANG Zi-yi, LI Yue-chan, YANG Wei. Screening of liquid fermentation medium of exopolysaccharide red aspergillus [J]. Journal of Tianjin Agricultural University, 2019, 26(1): 59-63.
[15]	LIU Yan, CHEN Xiao-qiang, WANG Xiao-hong, DENG Lin-xia, ZHANG Ya-he, WANG Jing-tai, WANG Dong-yuan. Effects of Tissue Culture Conditions on Polysaccharide Contents of Dendrobium Draconis [J]. Journal of Tianjin Agricultural University, 2015, 22(3): 32-35.