犬源弗氏柠檬酸杆菌的分离与鉴定

doi:10.19640/j.cnki.jtau.2019.01.010

Journal of Tianjin Agricultural University ›› 2019, Vol. 26 ›› Issue (1): 41-44.doi: 10.19640/j.cnki.jtau.2019.01.010

• Researches and Scientific Notes • Previous Articles Next Articles

Isolation and identification of Citrobacter freundii from dogs

ZHANG Xi-mo, LU Lu, LIU Yan-fei^{Corresponding Author}, YANG Jian-de^{Corresponding Author}

College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin 300384, China

Received:2018-05-27 Online:2019-03-20 Published:2019-10-09

Abstract

Abstract: In order to clarify the associated pathogens of companion animal suppurative diseases, uterine secretions from a patient pyometra dog at animal hospital affiliated to the college were collected sterilely for pathogen isolation and identification. The isolate was identified as Citrobacter freundii by bacterial isolation and culture, colony morphology observation and Gram’s staining observation, biochemical test and 16S rRNA gene analysis. Antibiotic susceptibility results showed that the isolated bacterium is resistant to Maddie and ampicillin, moderately sensitive to aztreonam, cefazolin, and cefoxitin, highly sensitive to cefoperazone, ceftriaxone, cefepime and other antibiotics, providing reference for prevention and related diseases control of companion animals.

Key words: dog, pyometra, Citrobacter freundii, isolation and identification, drug sensitive test

CLC Number:

S852.61

ZHANG Xi-mo, LU Lu, LIU Yan-fei, YANG Jian-de. Isolation and identification of Citrobacter freundii from dogs[J]. Journal of Tianjin Agricultural University, 2019, 26(1): 41-44.

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: http://xuebao.tjau.edu.cn/EN/10.19640/j.cnki.jtau.2019.01.010

http://xuebao.tjau.edu.cn/EN/Y2019/V26/I1/41

References

[1] 曹朕娇,丁诗华,凌空,等. 大鲵源弗氏柠檬酸杆菌的分离鉴定及胞外酶活性研究[J]. 西南大学学报,2016,38(5):13-18.
[2] 刘梦佳,任嘉瑞,张耀相,等. 弗氏柠檬酸杆菌的分离与鉴定[J]. 畜牧兽医杂志,2017,36(4):3-6.
[3] Shanks R M Q,Dashiff A,Alster J S,et al. Isolation and identification of a bacteriocin with antibacterial and antibiofilm activity from Citrobacter freundii[J].Archives of Microbiology,2012,194(7):67-70.
[4] 万彪,伍昌化,张文斌,等. 弗氏柠檬酸杆菌的分离鉴定与生物特性分析[J]. 贵州畜牧兽医,2015,39(3): 4-8.
[5] 薛巧,赵战勤,刘会胜,等. 弗氏柠檬酸杆菌对动物和人致病性研究[J]. 动物医学进展,2015,36(7):81-85.
[6] 徐文必,沈学文,肖啸,等. 引起犬子宫蓄脓细菌的分离鉴定[J]. 山东畜牧兽医,2012,33(3):2-5.
[7] Chaudhry W N,Haq I U,Andleeb S,et al.Characterization of a virulent bacteriophage LK1 specific for Citrobacter freundii isolated from sewage water[J]. Journal of Basic Microbiology,2014,54(6):531-541.
[8] 汲如芬. 保定地区犬子宫蓄脓的诊断及治疗研究[D]. 保定:河北农业大学,2014.
[9] 周加利. 柠檬酸杆菌O抗原基因簇RFLP 分型及5个O血清型基因簇的破译[D]. 天津:南开大学,2011.
[10] 刘广义. 156株弗氏柠檬酸杆菌感染的分布与耐药性分析[J]. 中华医院感染学杂志,2011,21(14):3040-3041.
[11] 王利,魏勇,龙钟明,等. 鲫鱼弗氏柠檬酸杆菌的耐药表型及耐药基因检测[J]. 黑龙江畜牧兽医,2012(15):14-16.
[12] 曹朕娇. 大鲵源弗氏柠檬酸杆菌的分离鉴定、敏感药物筛选及耐药性分析[D]. 重庆:西南大学,2015.
[13] 冯娇. 质粒介导的多重耐药性弗氏柠檬酸杆菌耐药机制研究[D]. 长春:吉林大学,2015.
[15] 胡秀彩,王艺,吕爱军. 弗氏柠檬酸杆菌的分离鉴定与PCR-SSCP分析[J]. 微生物杂志,2011,31(4):12-18.
[16] 刘淑敏,杜娜,刘嘉欣,等. 2012年至2014年耐碳青霉烯类弗氏柠檬酸杆菌的分布与耐药性[J]. 2016,37(8):35-38.

[1]	Chen Jiale, He Jinling, Du Rongqi, Li Danruo, Jin Tianming, Zhang Dongchao. Isolation, identification, and pathogenicity analysis of Escherichia coli in chicken [J]. Journal of Tianjin Agricultural University, 2023, 30(5): 23-29.
[2]	Pang Yue, Guo Meiling, Liu Xinrui, Xue Shuxia, Sun Jinsheng. Identification and pathogenicity analysis of Aeromonas veronii from Cyprinus carpio var. koi [J]. Journal of Tianjin Agricultural University, 2022, 29(4): 36-42.
[3]	Li Jianjun, Li Jiao, Lian Hong, Wang Lihui, Ding Qiaoling. Diagnosis and treatment of bladder calculi with giant cystomyoma in a dog [J]. Journal of Tianjin Agricultural University, 2022, 29(3): 43-46.
[4]	Ding Qiaoling, Liu Shuyue, Meng Ze, Li Jianjun. Isolation, identification and drug resistance of canine Staphylococcus pseudointermediate [J]. Journal of Tianjin Agricultural University, 2021, 28(3): 42-45.
[5]	DU Han-yu, GE Cheng-fei, WANG Li-li, HU Ye. Isolation, identification, and characterization of Bacillus subtilis [J]. Journal of Tianjin Agricultural University, 2020, 27(3): 53-56.
[6]	LIU Yan-fei, CUI Yao-xin, LIU Jun-hua, YANG Jian-de. Research advance on pyometra in dogs and cats [J]. Journal of Tianjin Agricultural University, 2020, 27(3): 86-88.
[7]	LUO Fu-li, NIU Yu-chen, HU Xiu-cai, LÜ Ai-jun, YE Ming-hao, SUN Jing-feng. Clinical signs and histochemical observation of Citrobacter freundii disease in turtle（Trachemys scripta elegans） [J]. Journal of Tianjin Agricultural University, 2020, 27(2): 44-47.
[8]	DU Rong-qi, Ren Jun, QIAO Qi, ZHANG Qing, LIU Yan-fei, YANG Jian-de. Isolation and identification of two hemolytic strains of canine Escherichia coli [J]. Journal of Tianjin Agricultural University, 2020, 27(1): 61-64.
[9]	SUN Xiao-li, GAO Jian, PAN Le-xin, HU Tao, MENG Bin, JIN Tian-ming. Isolation and identification and drug sensitivity test of Escherichia coli of pigs [J]. Journal of Tianjin Agricultural University, 2019, 26(2): 57-60.
[10]	JIN Xiang, XIE En-jie, LIU Zhe, DENG Qi-jun, LAN Tian-xin, HU De-bao, MA Ji-fei, GUO Liang, PU Lei. Treatment and nursing of bladder calculi and pyometra in pet dogs [J]. Journal of Tianjin Agricultural University, 2019, 26(1): 45-49.
[11]	LI Xin, MENG Jia-li, SHANG Cui-ling, LI Dan, LAN Zhao-yu, WANG Xiao-yue, JIN Tian-ming. Epidemiological investigation of canine dermatosis in a pet clinic in Tianjin [J]. Journal of Tianjin Agricultural University, 2019, 26(1): 50-53.
[12]	WANG Yu-hui, LI Xin, SUN Xiao-li, MENG Jia-li, SHANG Cui-ling, LIN Jing, JIN Tian-ming. The isolation and identification of pathogen and drug susceptibility test for Actinobacillus pleuropneumoniae [J]. Journal of Tianjin Agricultural University, 2018, 25(4): 33-37.
[13]	HAN Yu-xi, JIANG Xuan, GE Xiu-guo, AN Xiao-kang, ZHU Zi-da, ZHAO Rui-li, MA Ji-fei, LI Yue, WANG Cong, XU Liang-lin. The epidemiological investigation and pathogenicity study on Cyniclomyces guttulatus of police dogs in Tianjin area [J]. Journal of Tianjin Agricultural University, 2018, 25(4): 44-47.
[14]	ZHU Kun-yuan, WU Lan, LIU Yan-fei, YANG Jian-de. Isolation, identification and susceptibility analysis of Bacillus pyocyaners from a canine pyometra [J]. Journal of Tianjin Agricultural University, 2018, 25(3): 47-51.
[15]	JIANG Xuan, LIU Meng-yue, FU Chao, MA Ji-fei, ZHAO Rui-li, SHI Qing-qing, HU Ye, CUI Jun. Isolation and identification of enterotoxigenic Escherichia coli from large scale pig farms in Tianjin area [J]. Journal of Tianjin Agricultural University, 2018, 25(3): 52-56.

Isolation and identification of Citrobacter freundii from dogs

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments