Abstract: Flavonoid ingredients are abundant in effective extracts from Radix angelicae sinensis with bioactivities of anti-aging and scavenging free radicals through topical administration. Aiming at these characteristics, ointment was prepared for extracts from Radix angelicae sinensis with water-in-oil（W/O）type. At the same time, orthogonal screening was used in formulation selection in order to obtain stable ointment formulation with preferred skin-feeling. In this investigation, flavonoid ingredients were acquired through ultrasonic extraction firstly. Afterwards, comprehensive scoring of homogeneity, extensibility, centrifugal stability and cold/heat resistance was set as the evaluation indicator to obtain the optimal proportion of oil phase, emulsifier and coemulsifier. In this ointment formulation, Span-60 and Tween-80 were used as emulsifier and coemulsifier respectively, and lanolin/vaselin/stearic were used as muti-compositional oil phase with quality ratio of 1∶3∶4. After prepared under the optimal formulation, in which the mass percentage of oil phase, Span-60 and Tween-80 were 35.0%, 4.0% and 4.5% respectively, stable ointment was acquired in oil-in-water type. This study provides the basis for further application of Radix angelicae sinensis extracts ointment.

Key words: extract from Radix angelicae sinensis, orthogonal screening, ointment, formulation selection