天津地区规模化生猪场产肠毒素大肠杆菌的分离鉴定

doi:10.19640/j.cnki.jtau.2018.03.011

Journal of Tianjin Agricultural University ›› 2018, Vol. 25 ›› Issue (3): 52-56.doi: 10.19640/j.cnki.jtau.2018.03.011

• Researches and Scientific Notes • Previous Articles Next Articles

Isolation and identification of enterotoxigenic Escherichia coli from large scale pig farms in Tianjin area

JIANG Xuan, LIU Meng-yue, FU Chao, MA Ji-fei, ZHAO Rui-li^{Corresponding Author}, SHI Qing-qing, HU Ye, CUI Jun

College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin 300384, China

Received:2018-04-27 Online:2018-09-20 Published:2019-11-12

Abstract

Abstract: Escherichia coli with special serotype has different degrees of pathogenicity to human and animal intestines, among which enterotoxigenic Escherichia coli（ETEC）is more serious. Rectal swabs samples of 15 diarrhoea pigs in Tianjin area were collected and inoculated with MH, MacConkey Agar and eosin-methylene blue agar for bacterial culture and Gram staining microscopy.The colonies with different shape on the medium were tested for biochemical tests, 16S rRNA sequencing and BLAST analysis. The homology rate of 10 strains of Enterobacteriaceae and Escherichia coli was 99.6%. The specific primers of Escherichia coli enterotoxin ST1, ST2, LT1 and IR1 were synthesized. PCR results showed that 3 strains of Escherichia coli producing LT1 toxin, LT1 and ST1 toxin, and ST1 enterotoxin were isolated. The antimicrobial susceptibility test of isolated strains of enterotoxin LTI and ST1 showed that enterotoxigenic Escherichia coli was sensitive to neomycin, clindamycin, gentamicin, penicillin, ceftriaxone, amikacin, cefuroxime and florfenicol, which provided a basis for the monitoring and prevention of ETEC.

Key words: enterotoxigenic Escherichia coli（ETEC）, diarrhea, isolation and identification, 16S rRNA

CLC Number:

S852.611

JIANG Xuan, LIU Meng-yue, FU Chao, MA Ji-fei, ZHAO Rui-li, SHI Qing-qing, HU Ye, CUI Jun. Isolation and identification of enterotoxigenic Escherichia coli from large scale pig farms in Tianjin area[J]. Journal of Tianjin Agricultural University, 2018, 25(3): 52-56.

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Isolation and identification of enterotoxigenic Escherichia coli from large scale pig farms in Tianjin area

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