Journal of Tianjin Agricultural University ›› 2019, Vol. 26 ›› Issue (3): 62-67.doi: 10.19640/j.cnki.jtau.2019.03.014

• Researches and Scientific Notes • Previous Articles     Next Articles

Effects of enrofloxacin on the physiology and biochemistry of Amphiprion ocellaris

GAO Hong-wei1,2,3, HU Jing2,3,4, YANG Rui2,3,4, ZHOU Sheng-jie2,3,4, YU Gang2,3,4, MA Zhen-hua2,3,4   

  1. 1. Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China;
    2. Tropical Aquaculture Research and Development Centre, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Sanya 572018, Hainan Province, China;
    3. Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture, Guangzhou 510300, China;
    4. Sanya Tropical Fisheries Research Institute, Sanya 572018, Hainan Province, China
  • Received:2019-03-16 Online:2019-09-30 Published:2019-09-30

Abstract: To study the effects of different concentrations of enrofloxacin treatments on physiology and biochemistry of A. ocellaris, enrofloxacin soaking experiments(0, 5, 10 mg/L)were performed on three groups of A. ocellaris. The results showed that among the digestive enzymes activities, enrofloxacin had the most significant effect on trypsin and pepsin, followed by lipase and amylase. The order of enrofloxacin on the liver immune enzymes of the individuals was glutathione peroxidase(GSH)>alkaline phosphatase(AKP), lysozyme(LZM), glutathione S-transferase(GST)>superoxide dismutase(SOD), catalase(CAT), peroxidase(POD). The influence of oxidation products is more significant in lipid peroxide(LPO )than malondialdehyde(MDA). Enrofloxacin had an effect on the activities of various enzymes and the content of oxidized products in A. ocellaris, and the effect of soaking treatment had significant difference(P<0.05). This result is of great significance for the disease treatment of the A. ocellaris.

Key words: Amphiprion ocellaris, enrofloxacin, immune enzymes, digestive enzymes, oxidation products

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