河流弧菌B7-1羧肽酶基因的克隆与功能鉴定

doi:10.19640/j.cnki.jtau.2022.02.004

Journal of Tianjin Agricultural University ›› 2022, Vol. 29 ›› Issue (2): 18-21.doi: 10.19640/j.cnki.jtau.2022.02.004

• Researches and Scientific Notes • Previous Articles Next Articles

Cloning and functional identification of Vibrio fluvialis B7-1 carboxylpeptidase gene

Wei Songsong, Xie Xiaodong, Chen Shuaijun, Huang Haidong, Yang Hongpeng, Wu Jiang^{Corresponding Author}

College of Agronomy and Resource Environment, Tianjin Agricultural University, Tianjin 300392, China

Received:2020-11-25 Online:2022-06-30 Published:2022-07-05

Abstract

Abstract: Carboxypeptidase is a type of exonuclease that can specifically cleave individual amino acids one by one from the c-terminus of a peptide chain. In recent years, carboxypeptidase has attracted more and more attention in both biological and medical industry. In this study, the whole genome was obtained from bacterial Vibrio fluvialis, and an upstream primer and a downstream primer were designed to amplify the target gene encoding carboxypeptidase. The amplified target gene was transferred into TOP10 E.coli for recombinant amplification, and then the target protein was expressed in E.coli BL-21. Qualitative and quantitative analysis of the carboxypeptidase expression revealed that the recombinant strain containing the carboxypeptidase gene of V. fluvialis had strong capability of expressing carboxypeptidase and strong expressed enzyme activity.

Key words: gene, carboxypeptidase, enzyme activity, Folin-phenol method

CLC Number:

Q786

Wei Songsong, Xie Xiaodong, Chen Shuaijun, Huang Haidong, Yang Hongpeng, Wu Jiang. Cloning and functional identification of Vibrio fluvialis B7-1 carboxylpeptidase gene[J]. Journal of Tianjin Agricultural University, 2022, 29(2): 18-21.

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Cloning and functional identification of Vibrio fluvialis B7-1 carboxylpeptidase gene

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