小头鲔幼鱼不同消化器官中消化酶活性分析

doi:10.19640/j.cnki.jtau.2023.04.006

Journal of Tianjin Agricultural University ›› 2023, Vol. 30 ›› Issue (4): 27-31.doi: 10.19640/j.cnki.jtau.2023.04.006

• Researches and Scientific Notes • Previous Articles Next Articles

Analysis of digestive enzyme activities in different digestive organs of Euthynnus affinis

Wang Wenwen^1,2, Hu Jing^1,2,3, Zhou Shengjie^1,2,3, Yang Rui^1,2,3, Ma Zhenhua^1,2,3

1. Tropical Fisheries Research and Development Center, South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences, Sanya 572018, Hainan Province, China;
2. Sanya Tropical Fisheries Research Institute, Sanya 572018, Hainan Province, China;
3. Key Laboratory of Efficient Utilization and Processing of Marine Fishery Resources of Hainan Province, Sanya 572018, Hainan Province, China

Received:2022-06-15 Online:2023-08-31 Published:2023-11-01

Abstract

Abstract: In order to explore the digestive enzyme activities in different digestive organs of Euthynnus affinis Juvenile fish under artificial breeding conditions, the activities of lipase, amylase and protease were tested. The optimum temperature for lipase, amylase, pepsin and trypsin are 30~50 ℃, 30~45 ℃, 30~60 ℃ and 30~50 ℃, respectively. The results showed that lipase activity and protease activity could be detected in the stomach, liver, foregut, midgut and hindgut, and amylase activity could be detected in the stomach, liver, pancreas, foregut, midgut and hindgut. The results showed that lipase activity, amylase activity and protease activity were high in liver; Amylase activity and protease activity in pancreas were significantly higher than those in other digestive organs. In the optimum temperature of digestive enzymes, different digestive enzymes could be detected, and their activities were not exactly the same in different digestive parts. The liver plays a major role in the digestion of lipase, amylase is secreted mainly by the pancreas, and the pancreas is also the main organ for protease metabolism. The results of this study will provide reference data for the follow-up artificial culture of Euthynnus affinis.

Key words: Euthynnus affinis, digestive organs, digestive enzyme activity

CLC Number:

S917.4

Wang Wenwen, Hu Jing, Zhou Shengjie, Yang Rui, Ma Zhenhua. Analysis of digestive enzyme activities in different digestive organs of Euthynnus affinis[J]. Journal of Tianjin Agricultural University, 2023, 30(4): 27-31.

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Analysis of digestive enzyme activities in different digestive organs of Euthynnus affinis

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