半滑舌鳎细胞分裂周期蛋白42基因克隆与结构预测

doi:10.19640/j.cnki.jtau.2022.03.007

Journal of Tianjin Agricultural University ›› 2022, Vol. 29 ›› Issue (3): 36-42.doi: 10.19640/j.cnki.jtau.2022.03.007

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Cloning and structure prediction of cell division cyclin 42 gene from Cynoglossus semilaevis

Liu Houfu¹, Tan Jing¹, Hu Xiucai¹, Guan Zhenguo², Lü Aijun^{1,Corresponding Author}, Sun Jingfeng¹

1. Tianjin Key Laboratory of Aqua-Ecology and Aquaculture, College of Fisheries, Tianjin Agricultural University, Tianjin 300392, China;
2. S&E Burgeoning Biotechnology（Tianjin）Co., Ltd., Tianjin 300383, China

Received:2020-07-05 Online:2022-09-30 Published:2022-10-10

Abstract

Abstract: Cdc42 is a subclass of Rho superfamily enzymes that regulates cytoskeleton and actin activity, and participates in immune and inflammatory responses. In this study, the specific primers were designed for Cdc42 gene（CsCdc42）of Cynogl ossus semilaevis, and its cloning and structure prediction were performed by RT-PCR and bioinformatics methods. The results showed that the fragment of CsCdc42 gene was 847 bp in length, encoding 191 amino acids（aa）, and contained 7 epitopes （27-28, 61-63, 90-92, 120-122, 131-133, 164-166 and 181-183 aa）, one RHO domain（6-179 aa）, ATP/GTP binding site and acylation site, respectively. The protein structure was predicted by helix（30.9%）, β-fold（24.6%）and irregular coil（44.5%）. The results of sequence alignment showed that the homology of amino acid sequences of CsCdc42 was 99.43%, 97.91%, 95.81%, 94.76% and 91.10%, respectively, for zebrafish（Danio rerio）, medaka（Oryzias latipes）, rainbow trout（Oncorhynchus mykiss）, turbot （Scophthalmus maximus）and shrimp（Penaeus vannamei）. Phylogenetic tree analysis showed that Cdc42 was closely related to zebrafish（Danio rerio）, human（Homo sapiens）and several marine fishes, and naturally grouped into one cluster. These results provide a scientific reference for further study of the function of cyclin-related genes in Cynoglossus semilaevis.

Key words: Cynoglossus semilaevis, Cdc42, gene cloning, structure prediction

CLC Number:

S917.4

Liu Houfu, Tan Jing, Hu Xiucai, Guan Zhenguo, Lü Aijun, Sun Jingfeng. Cloning and structure prediction of cell division cyclin 42 gene from Cynoglossus semilaevis[J]. Journal of Tianjin Agricultural University, 2022, 29(3): 36-42.

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Cloning and structure prediction of cell division cyclin 42 gene from Cynoglossus semilaevis

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