拟南芥LHY基因超表达与成花转变

doi:10.19640/j.cnki.jtau.2021.02.005

Journal of Tianjin Agricultural University ›› 2021, Vol. 28 ›› Issue (2): 25-30.doi: 10.19640/j.cnki.jtau.2021.02.005

• Researches and Scientific Notes • Previous Articles Next Articles

The floral transition of LHY overexpression plants in Arabidopsis thaliana

Yin Sheng, Fu Yu, Long Hong^*

College of Horticulture and Landscape, Tianjin Agricultural University, Tianjin 300392, China

Received:2020-04-26 Online:2021-06-30 Published:2021-07-26

Abstract

Abstract: The floral transition in higher plants is regulated by multiple gene network. The transition of plants from vegetative growth to reproductive growth is related to the biological rhythm of plant development. However, the regulatory role of circadian clock-related genes in it is still unclear. By constructing the overexpression vector pCAMBIA-3301-35S::LHY, and the following genetic transformation, the effects of overexpression of circadian clock-related gene LHY on floral transition were analyzed with overexpression plants. The results showed that LHY overexpression plants had 18 rosette leaves, while only 12 existed in wild-type plants. LHY overexpression plants had visible flower buds on 33rd day, while wild-type plants were on the 20th day. The overexpressed plants showed late flower phenotype. The anatomical structure analysis of the shoot apical meristem showed that the growth cone of LHY overexpression plants continued to grow and develop to a prominent height on the 19th day (compared to 15th day in wild-type plants), with multiple layers of tunica, corpus and pith meristem zone cells. These indicated that overexpression plants had delayed vegetative growth and maturity, leading to late flowering. The results of qRT-PCR on the expression level of miR156 and its target gene SPL3 showed that LHY overexpression plants regulated the floral transition by down-regulating and up-regulating the expression levels of miR156 and SPL3 during plant growth.

Key words: Arabidopsis thaliana, floral transition, LHY gene, overexpression

CLC Number:

Q756

Yin Sheng, Fu Yu, Long Hong. The floral transition of LHY overexpression plants in Arabidopsis thaliana[J]. Journal of Tianjin Agricultural University, 2021, 28(2): 25-30.

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The floral transition of LHY overexpression plants in Arabidopsis thaliana

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