刚地弓形虫SRS29C截短型基因的克隆及原核表达质粒的构建

doi:10.19640/j.cnki.jtau.2021.03.011

Journal of Tianjin Agricultural University ›› 2021, Vol. 28 ›› Issue (3): 50-55.doi: 10.19640/j.cnki.jtau.2021.03.011

• Researches and Scientific Notes • Previous Articles Next Articles

Cloning and construction of prokaryotic expression plasmid of truncated SRS29C gene of Toxoplasma gondii

Yuan Di¹, Shao Yi¹, Zhang Tongyao¹, Jiang Fushan¹, Li Xiurong², Song Qiqi^{1,Corresponding Author}

1. Tianjin Key Laboratory of Agricultural Animal Breeding and Healthy Husbandry, College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin 300392, China;
2. Agricultural and Animal Product Quality and Safety Center of Linhe district, Bayannaoer 015000, Inner Mongolia Autonomous Region, China

Received:2020-04-14 Online:2021-09-30 Published:2021-10-07

Abstract

Abstract: In order to construct the prokaryotic expression plasmid of Toxoplasma gondii SRS29C gene, the signal peptide of SRS29C gene was predicted by using SignalP-5.0 software, the sequence of the signal peptide of SRS29C gene was removed, the truncated gene of SRS29C was amplified and cloned, and then the prokaryotic expression plasmids were constructed. The results showed that the first 51 amino acids of SRS29C were the signal peptide region, so by cutting off the signal peptide, the 966 bp SRS29C truncated gene fragment (SRS29Ccut) was successfully obtained, and the recombinant plasmids pET-28a-SRS29Ccut and pET-32a-SRS29Ccut were successfully constructed. This study may lay a foundation for further study on the biological function and immune characteristics of the protein of Toxoplasma gondii SRS29C.

Key words: Toxoplasma gondii, SRS29C, truncated gene, prokaryotic expression plasmid

CLC Number:

S855.9

Yuan Di, Shao Yi, Zhang Tongyao, Jiang Fushan, Li Xiurong, Song Qiqi. Cloning and construction of prokaryotic expression plasmid of truncated SRS29C gene of Toxoplasma gondii[J]. Journal of Tianjin Agricultural University, 2021, 28(3): 50-55.

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Cloning and construction of prokaryotic expression plasmid of truncated SRS29C gene of Toxoplasma gondii

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