白藜芦醇对ZEN诱导的猪小肠上皮细胞损伤的作用效果研究

doi:10.19640/j.cnki.jtau.2023.03.009

Journal of Tianjin Agricultural University ›› 2023, Vol. 30 ›› Issue (3): 47-52.doi: 10.19640/j.cnki.jtau.2023.03.009

• Researches and Scientific Notes • Previous Articles Next Articles

Effects of resveratrol on zearalenone induced injury of porcine intestinal epithelial cells

Han Jing’e, Wang Huanhuan, Li Pengcheng, She Fuze, Li Xiaoyu, Qin Shunyi^{Corresponding Author}

Tianjin Key Laboratory of Agricultural Animal Breeding and Healthy Husbandry, College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin 300392, China

Received:2022-04-25 Online:2023-06-30 Published:2023-09-06

Abstract

Abstract: The purpose of this study was to investigate the effect of resveratrol on the injury of porcine small intestinal epithelial cells（IPEC-J2 cells）induced by zearalenone（Zen）. The experimental groups were: Group C（control group）, group Z（25 µg/mL Zen）, group ZR1（25 µg/mL Zen+10 µmol/L resveratrol）, group ZR2（25 µg/mL Zen+20 µmol/L resveratrol）, group ZR3（25 µg/mL Zen+30 µmol/L resveratrol）. The cell viability, the activity of lactate dehydrogenase（LDH）, the level of antioxidant enzymes and the mRNA expression of apoptosis related genes such as caspase 3, caspase 9, Bcl-2 and Bax were measured. The results showed that the cell viability, SOD activity, T-AOC level and Bcl-2 gene mRNA expression level in group Z were significantly lower than those in group C（P<0.01）. The cell viability, SOD activity, T-AOC level and Bcl-2 gene mRNA expression level in group ZR2 and ZR3 were significantly higher than those in group Z or group ZR1（P<0.01 or P<0.05）. The LDH activity, MDA level, Capase-3, Capase-9 and Bax gene mRNA expression levels in group Z were significantly higher than those in group C（P<0.01）. The LDH activity, MDA level, Capase-3, Capase-9 and Bax gene mRNA expression levels in group ZR2 and ZR3 were lower or significantly lower than those in group Z or group ZR1（P<0.01 or P<0.05）. The LDH activity and Bax gene mRNA expression levels in ZR1 group were lower than those in group Z（P<0.01）. The results indicated that resveratrol could alleviate the changes of cell viability, LDH activity, antioxidant function and apoptosis related gene mRNA expression of IPEC-J2 cells induced by Zen.

Key words: resveratrol, zearalenone, porcine intestinal epithelial cells, cell viability, lactate dehydrogenase activity, antioxidant function, apoptosis gene

CLC Number:

S816.7

Han Jing’e, Wang Huanhuan, Li Pengcheng, She Fuze, Li Xiaoyu, Qin Shunyi. Effects of resveratrol on zearalenone induced injury of porcine intestinal epithelial cells[J]. Journal of Tianjin Agricultural University, 2023, 30(3): 47-52.

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Effects of resveratrol on zearalenone induced injury of porcine intestinal epithelial cells

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