溶解氧对半滑舌鳎呼吸频率、生理生化指标和组织结构的影响

doi:10.19640/j.cnki.jtau.2024.01.010

Journal of Tianjin Agricultural University ›› 2024, Vol. 31 ›› Issue (1): 54-60.doi: 10.19640/j.cnki.jtau.2024.01.010

• Researches and Scientific Notes • Previous Articles Next Articles

Effects of dissolved oxygen on respiratory rate, physiological and biochemical parameter and tissue structure of Cynoglossus semilaevis

Nie Guoliang¹, Wang Qingkui^{1,Corresponding Author}, Zhao Honghao¹, Wang Zhenhui², Fu Jiandong², Li Qiang^{1,Corresponding Author}

1. College of Fisheries, Tianjin Agricultural University, Tianjin 300392, China;
2. Tianjin Haifa Zhenpin Industrial Development Co., Ltd., Tianjin 300450, China

Received:2023-05-11 Online:2024-02-29 Published:2024-04-02

Abstract

Abstract: In order to understand the effects of dissolved oxygen changes on Cynoglossus semilaevis , which often occurs in circulating water culture systems with low and high oxygen, this experiment examined the effects of low dissolved oxygen（3 mg/L）, control（8 mg/L）, and high dissolved oxygen（14 mg/L, 20 mg/L）conditions on the respiration, physiological and biochemical parameter, and tissue structure of Cynoglossus semilaevis. The results showed that respiratory rate increased significantly in the hypoxic group and decreased significantly in the hyperoxic group within 24 h（P<0.05）At day 7, the respiratory rate of the low oxygen group was not significantly different from that of the control group（P>0.05）, while the respiratory rate of the high oxygen group decreased significantly with the increase of dissolved oxygen（P<0.05）. After the 14th day, the respiratory rate of the low oxygen group was not significantly different from that of the control group, while the respiratory rate of the high oxygen group decreased significantly with the increase of dissolved oxygen（P<0.05）. Both the hypoxic and hyperoxic groups showed proliferation of epithelial cell layers compared to the control group, and the gill tissue in the hyperoxic group showed more vacuolation than the control group in the Cynoglossus semilaevis. In the hypoxic group, liver tissue structure was abnormal, with extensive loose edema and unclear cell outline, while the hepatocytes in the hyperoxic group had clear outline and red stained cytoplasm, but no obvious degeneration was observed. The number of erythrocytes and hemoglobin content of blood in the hypoxic group increased compared with the control group（P>0.05）, and the ratio of erythrocyte volume decreased compared with the control group（P>0.05）. The erythrocyte count, hemoglobin content and erythrocyte specific volume were higher in the hyperoxia group than in the control group（P>0.05）. Plasma MDA content, SOD activity and CAT activity were significantly higher in the hypoxic and hyperoxic groups than in the control group（P<0.05）. In conclusion, both hypoxia and hyperoxia caused stress on Cynoglossus semilaevis, and the stress of hypoxia was more obvious on Cynoglossus semilaevis.

Key words: Cynoglossus semilaevis, dissolved oxygen, respiratory rate, blood indicators, tissue sections, enzyme activity

CLC Number:

S917.4

Nie Guoliang, Wang Qingkui, Zhao Honghao, Wang Zhenhui, Fu Jiandong, Li Qiang. Effects of dissolved oxygen on respiratory rate, physiological and biochemical parameter and tissue structure of Cynoglossus semilaevis[J]. Journal of Tianjin Agricultural University, 2024, 31(1): 54-60.

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References

[1] 王维政,曾泽乾,黄建盛,等. 低氧胁迫对军曹鱼幼鱼生长、血清生化和非特异性免疫指标的影响[J]. 海洋学报,2021,43(2):49-58.
[2] VALÉRIE M,PICHAVANT K,BOEUF G,et al. Effects of hypoxia on respiratory physiology of turbot,Scophthalmus maximus[J]. Fish Physiology & Biochemistry,2000,22(1):51-59.
[3] PAPOUTSOGLOU S E,KARAKATSOULI N,PIZZONIA G,et al.Effects of rearing density on growth,brain neurotransmitters and liver fatty acid composition of juvenile white sea bream Diplodus sargus L[J]. Aquaculture Research,2006,37(1):87-95.
[4] NIKINMAA M,SOIVIO A.Blood oxygen transport of hypoxic Salmo gairdneri[J]. Journal of Experimental Zoology,1982,219(2):173-178.
[5] 钱辰颖,郑国栋,陈杰,等. 溶解氧对团头鲂耐低氧新品系F₅代的鳃组织形态及各组织酶活性的影响[J]. 渔业科学进展,2021,42(4):73-82.
[6] JØRUND S, GÖRAN E N. Plasticity of respiratory structures—Adaptive remodeling of fish gills induced by ambient oxygen and temperature[J]. Respiratory Physiology & Neurobiology,2006,154(1):241-251.
[7] ARU C,BLM C,BMA F,et al.Hypoxia-induced metabolic and antioxidant enzymatic activities in the estuarine fish Leiostomus xanthurus[J]. Journal of Experimental Marine Biology and Ecology,2002,279(1):1-20.
[8] SPENCE B C,LOMNICKY G A,HUGHES R M,et al.An ecosystem approach to salmonid conservation[M]. Corvallis:ManTech Environmental Research Services Corp,1996.
[9] 陈德举,强俊,陶易凡,等. 不同溶氧水平对吉富罗非鱼幼鱼生长、血液生化、脂肪酸组成及其抗海豚链球菌病的影响[J]. 淡水渔业,2019,49(4):83-89.
[10] PELSTER B,DECKER H.The Root effect-a physiological perspective[J]. Micron,2004,35(1):73-74.
[11] PROKEŠOVÁ M,GEBAUER T,MATOUŠEK J,et al. Effect of temperature and oxygen regime on growth and physiology of juvenile Salvelinus fontinalis×Salvelinus alpinus hybrids[J]. Aquaculture,2020,522:735119.
[12] LUSHCHAK V I,LUSHCHAK L P,MOTA A A,et al.Oxidative stress and antioxidant defenses in goldfish Carassius auratus during anoxia and reoxygenation[J]. American Journal of Physiology Regulatory,Integrative and Comparative Physiology,2001,280(1):100-107.
[13] ABDALLAH S J,THOMAS B S,JONZ M G.Aquatic surface respiration and swimming behaviour in adult and developing zebrafish exposed to hypoxia[J]. The Journal of Experimental Biology,2015,218(11):1777-1786.
[14] DHILLON R S,YAO L,MATEY V,et al.Interspecific differences in hypoxia-Induced gill remodeling in carp[J]. Physiological and Biochemical Zoology,2013,86(6):727-739.
[15] 李学军,金华,张艳红. 罗非鱼鱼苗气泡病的发生与预防[J]. 水利渔业,2004,24(1):63-64.
[16] 彭天辉,潘连德,唐绍林. 大口黑鲈慢性气泡病的组织病理观察以及水体分层对发病的影响[J]. 大连海洋大学学报,2013,28(6):578-584.
[17] EBEL W J,RAYMOND H L,MONAN G E,et al.Effect of atmospheric gas supersaturation caused by dams on salmon and steelhead trout of the snake and columbia rivers[M]. Washington:Northwest Fisheries Center Review,1975.
[18] VAL A,LESSARD J,RANDALL D.Effects of hypoxia on rainbow trout(Oncorhynchus mykiss):Intraerythrocytic phosphates[J]. Journal of Experimental Biology,1995,198(2):305-310.
[19] 沈晓民,童合一. 团头鲂血液指标与水环境的关系[J].生态学报,1991,11(1):92-94.
[20] 吴志昊,尤锋,王英芳,等. 低氧和高氧对大菱鲆幼鱼红细胞核异常及氧化抗氧化平衡的影响[J]. 上海海洋大学学报,2011,20(6):808-813.
[21] 秦勇. 细胞内ROS水平改变对细胞活性及相关信号传导途径的影响[D]. 杭州:浙江大学,2012.
[22] MCARLEY T J,SANDBLOM E,HERBERT N A.Fish and hyperoxia—From cardiorespiratory and biochemical adjustments to aquaculture and ecophysiology implications[J]. Fish and Fisheries,2021,22(2):324-355.
[23] 苏柯,张和森,肖保强,等. 封闭式循环海水系统大菱鲆高密度养殖研究[J]. 渔业现代化,2003(5):9-12.
[24] 裴雪莹. 杂交黄颡鱼“黄优1号”应对低氧胁迫的生理响应及基因表达研究[D]. 南京:南京师范大学,2020.
[25] 高云涛,高云红,李明月,等. 许氏平鲉低氧耐受能力及血液学和鳃组织学变化[J]. 水产学报,2023,47(9):40-51.
[26] 罗辉玉,吴水清,郑乐云,等. 溶解氧对线纹海马幼鱼氨氮耐受性的影响及氨氮胁迫下鳃、肝脏组织结构的变化[J]. 生态学杂志,2020,39(3):872-879.
[27] 钱辰颖. 低氧和高氧对团头鲂F₅新品系鳃组织形态变化及各组织酶活性的影响[D]. 上海:上海海洋大学,2020.
[28] BOSCH-BELMAR M,GIOMI F,RINALDI A,et al.Concurrent environmental stressors and jellyfish stings impair caged European sea bass(Dicentrarchus labrax)physiological performances[J]. Scientific Reports,2016,6:27929.
[29] SCHERZ-SHOUVAL R,ELAZAR Z.Regulation of autophagy by ROS physiology and pathology[J]. Trends Biochem Sci,2011,36(1):30-38.
[30] 张国松. 瓦氏黄颡鱼(Pelteobagrus vachelli)应对低氧胁迫的分子机制研究[D]. 南京:南京师范大学,2017.
[31] 杜开开. 水体中过饱和溶解气体释放因素探究[D]. 上海:上海海洋大学,2017.
[32] SHI X,ZHOU B.The role of Nrf2 and MAPK pathways in PFOS-induced oxidative stress in zebrafish embryos[J]. Toxicological Sciences,2010,115(2):391-400.
[33] 马粒雅,王闻,迟雯丹,等. 溶解氧变化对中华乌塘鳢酶活的影响及其低氧耐受力研究[J]. 安徽农业科学,2020,48(3):91-94.
[34] 吴鑫杰,陈楠,黄春筱,等. 低氧对团头鲂心肌细胞凋亡及抗氧化酶活性的影响[J]. 华中农业大学学报,2016,35(3):108-113.

Effects of dissolved oxygen on respiratory rate, physiological and biochemical parameter and tissue structure of Cynoglossus semilaevis

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