革胡子鲶干扰素α基因对维氏气单胞菌感染的应答分析

doi:10.19640/j.cnki.jtau.2021.03.012

Journal of Tianjin Agricultural University ›› 2021, Vol. 28 ›› Issue (3): 56-60.doi: 10.19640/j.cnki.jtau.2021.03.012

• Researches and Scientific Notes • Previous Articles Next Articles

Response of interferon α gene to Aeromonas veronii infection in Clarias gariepinus

Gu Chenkan, Wang Xiaomei^{Corresponding Author}, Li Zhuanzhuan, Feng Miaomiao, Chen Chengxun

Tianjin Key Laboratory of Aqua-Ecology and Aquaculture, College of Fisheries, Tianjin Agricultural University, Tianjin 300392, China

Received:2020-08-06 Online:2021-09-30 Published:2021-10-07

Abstract

Abstract: In this paper, real-time quantitative PCR technique was used to assess the expression profiles of interferon alpha（IFNα）gene in spleen, kidney, head kidney, gill, intestine and skin of Clarias gariepinus following an intraperitoneal injection of Aeromonas veroni. The results showed that the IFNα gene was expressed in all tissues of the healthy（control）individuals, and the highest expression was found in spleen, which was significantly higher than those in other tissues except kidney and skin. After A. veronii challenge during 3-96 h, the temporal expressions profiles of IFNα gene in the six tissues were similar, namely, transcripts were up-regulated firstly and then recovered back to the baseline, but the expression peaked at different time points. Furthermore, the expression levels of IFNα gene were different among the tissues at the same time points of post infection.

Key words: Clarias gariepinus, Aeromonas veronii, interferon α, gene expression, real-time quantitative PCR

CLC Number:

Gu Chenkan, Wang Xiaomei, Li Zhuanzhuan, Feng Miaomiao, Chen Chengxun. Response of interferon α gene to Aeromonas veronii infection in Clarias gariepinus[J]. Journal of Tianjin Agricultural University, 2021, 28(3): 56-60.

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Response of interferon α gene to Aeromonas veronii infection in Clarias gariepinus

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