三赞胶降解菌的筛选鉴定及降解产物的抗氧化活性分析

doi:10.19640/j.cnki.jtau.2019.04.009

Journal of Tianjin Agricultural University ›› 2019, Vol. 26 ›› Issue (4): 43-48.doi: 10.19640/j.cnki.jtau.2019.04.009

• Researches and Scientific Notes • Previous Articles Next Articles

Screening, identification of sanxan-degrading microorganisms and antioxidant activities of degradation product

CHENG Bin¹, LI Xiao-yan², PAN Yi-chen¹, FENG Xue¹, HUANG Hai-dong^{1, Corresponding Author}

1. College of Agronomy & Resources and Environment, Tianjin Agricultural University, Tianjin 300384, China;
2. College of Food Science and Bioengineering, Tianjin Agricultural University, Tianjin 300384, China

Received:2019-04-03 Published:2020-01-19

Abstract

Abstract: ：Sanxan-degrading strains were screened, which formed degrading band on medium plate with sanxan as the only carbon source. On the basis of polyphasic taxonomic study, strain JD05, JD08 and SZJ01 were considered to be Pseudomonas fluorescens, Bacillus megaterium and Aspergillus fumiga. Treated by the mixture fermentation liquid of these three strains with the temperature of 37 ℃ and 72 h, the viscosity of sanxan solution decreased by 79.6%. The molecular weight of polysaccharide was reduced to two components with different molecular weight distributions, with 1.9×10⁴ Da and 3.7×10³ Da, respectively. The antioxidant activity of sanxan degradation product increased, with the IC₅₀ of scavenging hydroxyl free radical 3.8 mg/mL and the IC₅₀ of scavenging superoxide anion 2.6 mg/mL, respectively.

Key words: sanxan, polysaccharide, degrading microorganisms, molecular weight, antioxidant activity

CLC Number:

Q939.97

CHENG Bin, LI Xiao-yan, PAN Yi-chen, FENG Xue, HUANG Hai-dong. Screening, identification of sanxan-degrading microorganisms and antioxidant activities of degradation product[J]. Journal of Tianjin Agricultural University, 2019, 26(4): 43-48.

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: http://xuebao.tjau.edu.cn/EN/10.19640/j.cnki.jtau.2019.04.009

http://xuebao.tjau.edu.cn/EN/Y2019/V26/I4/43

References

[1] Fialho A,Moreira L,Granja A,et al.Occurrence,production,and applications of gellan:current state and perspectives[J]. Appl Microbiol Biotechnol,2008,79:889-900.
[2] 黄海东,刘云,刘如林. 合成生物聚合物的重要微生物资源-鞘氨醇单胞菌[J]. 微生物学报,2009,49(5):561-567.
[3] García M C,Alfaro M C,Muñoz J.Rheology of sheared gels based on low acyl-gellan gum[J]. Food Sci Technol Int,2016,22(4):325-332.
[4] Haidong H,Wei W,Ting M,et al.Sphingomonas sanxanigenens sp. nov.,isolated from soil[J]. Int J Syst Evol Microbiol,2009,59(4):719-723.
[5] Haidong H,Mengmeng W,Hongpeng Y,et al.Structural and physical proterties of sanxan polysaccharide from Sphingomonas sanxanigenens[J]. Carbohyd Polym,2016,144:410-418.
[6] 张爱琳,黄海东,张亚运,等. 几种食品添加剂对鞘氨醇胶Ss凝胶特性的影响[J]. 天津农学院学报,2016,23(2):36-40.
[7] 文雯. 5种食品添加剂新品种公开征求意见涉及三赞胶、聚甘油蓖麻醇酸酯等[EB/OL]. (2018-12-18)[2019-04-01]. http://info.foodmate.net/reading/show-5699. html.
[8] 李晓雁,黄海东,李军. 三赞胶的降解及抗氧化活性的研究[J]. 食品工业科技,2013,34(20):99-101.
[9] 钟思恩,刘杨,曹纯洁,等. 三种多糖降解复合物体外抑制HeLa细胞的增殖作用[J]. 现代食品科技,2018,34(10):72-80.
[10] 许超,熊亚茹,卢明倩,等. 一株具有褐藻胶降解能力的海洋细菌的筛选鉴定及其多糖利用能力研究[J]. 生物技术通报,2017,33(4):198-204.
[11] 孙涛,熊小英,魏颖隽,等. 黄原胶降解及其抗氧化性能研究[J]. 天然产物研究与开发,2012,24(1):102-104.
[12] 杨杰,单岩,张庆,等. 黄原胶降解酶酶学性质的初步研究[J]. 食品科技,2010,35(4):25-29.
[13] 黄海东,王薇,马挺,等. 鞘氨醇单胞菌TP-3合成新型生物聚合物Ss的发酵条件优化[J]. 微生物学通报,2009,36(2):155-159.
[14] 东秀珠,蔡妙英. 常见细菌系统鉴定手册[M]. 北京:科学出版社,2001.
[15] Tamura K,Stecher G,Peterson D,et al.MEGA6:molecular evolutionary genetics analysis version 6.0[J]. Mol Biol Evol,2013,30:2725-2729.
[16] 符梦凡,赵一帆,阎卫东. 柱前衍生化HPLC法分析枸杞多糖中单糖组成[J]. 食品科学,2018,39(18):186-191.
[17] 江丽丽,张庆,徐世艾. 黄原胶降解的研究进展[J]. 微生物学通报,2008,35(8):1292-1296.

Screening, identification of sanxan-degrading microorganisms and antioxidant activities of degradation product

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	Li Xiang, Zhang Pingping, Zhang Huan, Zhang Yangyang, Lan Zhixiang. Optimization of extraction process and preliminary structural analysis of polysaccharide from Xiuzhen mushroom by response surface methodology [J]. Journal of Tianjin Agricultural University, 2024, 31(2): 38-44.
[2]	Tian Tian, Hou Haifeng, Li Xiaoyan, Quan Guizhi, Lai Wenmei, Huang Haidong. Study on influence factors of the suspension performance of sanxan [J]. Journal of Tianjin Agricultural University, 2024, 31(2): 45-50.
[3]	Zhang Yang, Lu Hegang, You Pengzhi, Li Xiaoyan, Huang Haidong. Screening, identification of strain ZYRH-19 and characteristics of its product welan gum [J]. Journal of Tianjin Agricultural University, 2024, 31(1): 36-40.
[4]	Zhang Huan, Zhang Pingping, Li Xiang, Tong Xiaohui, Wang Feng, Zhao Chenchen. Effect of enzymatic hydrolysis on soluble dietary fiber and antioxidant activity in old asparagus stalks [J]. Journal of Tianjin Agricultural University, 2023, 30(6): 11-15.
[5]	Yang Zongfen, Wang Min, Wang Zhenli, Wang Haifeng, Wang Junbin. Study on antioxidant and antibacterial activity of polysaccharide extract in Mesembryanthemum crystallinum [J]. Journal of Tianjin Agricultural University, 2023, 30(1): 1-5.
[6]	Wang Zhenli, Wang Junbin, Wang Min, Yang Zongfen, Wang Haifeng. Study on extraction optimization of polyphenols from Mesembryanthemum crystallinum and its antioxidant and bacteriostatic activities [J]. Journal of Tianjin Agricultural University, 2022, 29(3): 23-28.
[7]	Wei Yating, Zhang Jin, Pan Jing, Li Zhaojie, Yang Hongpeng. Study on extraction technology of soybean meal polysaccharide by compound enzyme [J]. Journal of Tianjin Agricultural University, 2022, 29(2): 27-31.
[8]	Wang Min, Wang Junbin, Wang Zhenli, Yang Zongfen, Wang Haifeng. Study on the optimization of extraction and the antioxidant activity of total flavonoids in Mesembryanthemum crystallinum [J]. Journal of Tianjin Agricultural University, 2022, 29(1): 33-36.
[9]	Xu Xianmei, Cai Fenglong, Wu Fengming, Li Liuan, Zhang Jianbin. Study on the preparation of pullulan polysaccharide nanoparticles by dialysis [J]. Journal of Tianjin Agricultural University, 2021, 28(4): 23-26.
[10]	Guo Yi, Li Yiteng, Wu Haiqing, Wang Bujiang, Xiao Ping, Liu Jinfu. Effect of bitter melon saponins with pumpkin polysaccharides or buckwheat flavones on glucose and lipid metabolism in hyperlipidemia rats [J]. Journal of Tianjin Agricultural University, 2021, 28(2): 51-55.
[11]	Guo Yi, Yang Xuan, Xiao Ping. Study on extraction process of quinoa polysaccharide by response surface methodogy [J]. Journal of Tianjin Agricultural University, 2020, 27(4): 43-48.
[12]	FENG Zi-yao, ZHANG Ping-ping, ZHANG Hong-ying, LIN Xiang-jin, CHUAI Dong-hua, ZHANG Sheng-kang. Study on processing technology optimization of compound Pleurotus-eryngii tablets [J]. Journal of Tianjin Agricultural University, 2020, 27(3): 62-65.
[13]	GUO Yi, YU Jing-jing, XIAO Ping. Study on the technology of extracting polysaccharides from Radix Puerariae by Ultra-high pressure method [J]. Journal of Tianjin Agricultural University, 2020, 27(3): 66-69.
[14]	KANG Kun, WANG Jun-bin, WANG Hai-feng, WANG Feng-tang, WANG Yao-guo, WANG Hao. Optimization of extraction and antioxidant activity of total flavonoids in pecan shell [J]. Journal of Tianjin Agricultural University, 2020, 27(3): 74-78.
[15]	LI Yue-chan, ZHANG Zi-yi, WEI Xue-yuan, CHANG Li, YANG Wei. Optimization of conditions for the production of exopolysaccharides by Monascus and preliminary detection of efficacy activity [J]. Journal of Tianjin Agricultural University, 2020, 27(2): 79-83.