冰菜总黄酮的提取优化及抗氧化活性研究

doi:10.19640/j.cnki.jtau.2022.01.007

Journal of Tianjin Agricultural University ›› 2022, Vol. 29 ›› Issue (1): 33-36.doi: 10.19640/j.cnki.jtau.2022.01.007

• Researches and Scientific Notes • Previous Articles Next Articles

Study on the optimization of extraction and the antioxidant activity of total flavonoids in Mesembryanthemum crystallinum

Wang Min^a, Wang Junbin^{a,b,Corresponding Author}, Wang Zhenli^a, Yang Zongfen^a, Wang Haifeng^c

Tianjin Agricultural University, a. College of Basic Sciences, b. Chemistry Experiment Teaching Center, c. Center for Agricultural Analysis and Measurement, Tianjin 300392, China

Received:2021-03-11 Online:2022-03-31 Published:2022-04-14

Abstract

Abstract: Mesembryanthemum crystallinum is a new type of high-value health vegetable introduced into China in recent years, which is increasingly popular in the public. In this study, the M. crystallinum was used as the material to extract total flavonoids by ultrasonic-assisted extraction. Taking the extraction rate of total flavonoids as the evaluation index, on the basis of single-factor test results, the orthogonal extraction method was used to optimize the extraction process conditions, and the scavenging ability of total flavonoids from the M. crystallinum to 1,1-diphenyl-2-picrylhydrazyl（DPPH）and superoxide anion free radical was estimated. The results showed that the extraction rate of the total flavonoids reached 4.91% under the conditions of ethanol concentration of 60%, material-liquid ratio of 1∶15, extraction temperature of 50 ℃ and extraction time of 120 minutes. Within the concentration range of 0.02-0.10 mg/mL, the total flavonoids extract of M. crystallinum had antioxidant activity, and the higher the concentration of the extract, the higher the antioxidant capacity.

Key words: Mesembryanthemum crystallinum, total flavonoids, orthogonal design, antioxidant activity

CLC Number:

TS202.3

Wang Min, Wang Junbin, Wang Zhenli, Yang Zongfen, Wang Haifeng. Study on the optimization of extraction and the antioxidant activity of total flavonoids in Mesembryanthemum crystallinum[J]. Journal of Tianjin Agricultural University, 2022, 29(1): 33-36.

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Study on the optimization of extraction and the antioxidant activity of total flavonoids in Mesembryanthemum crystallinum

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