Journal of Tianjin Agricultural University ›› 2020, Vol. 27 ›› Issue (3): 74-78.doi: 10.19640/j.cnki.jtau.2020.03.017

• Researches and Scientific Notes • Previous Articles     Next Articles

Optimization of extraction and antioxidant activity of total flavonoids in pecan shell

KANG Kun1, WANG Jun-bin1,2,Corresponding Author, WANG Hai-feng3, WANG Feng-tang1, WANG Yao-guo1, WANG Hao1   

  1. 1. College of Basic Sciences, Tianjin Agricultural University, Tianjin 300384, China;
    2. Chemistry Experiment Teaching Center, Tianjin Agricultural University, Tianjin 300384, China;
    3. Center for Agricultural Analysis and Measurement, Tianjin Agricultural University, Tianjin 300384, China
  • Received:2020-04-13 Online:2020-10-10 Published:2020-10-10

Abstract: In this study, the pecan shell was used as the material to extract total flavonoids by ultrasonic-assisted extraction. Taking the extraction rate of total flavonoids as the evaluation index, on the basis of single-factor test results, the orthogonal extraction method was used to optimize the extraction process conditions, and the scavenging ability of total flavonoids from the pecan shell to 1,1-diphenyl-2-picrylhydrazyl(DPPH)and superoxide anion free radical was estimated. The results showed that the extraction rate of the total flavonoids reached the highest under the conditions of ethanol concentration of 65%, material-liquid ratio of 1∶30, extraction temperature of 40 ℃ and extraction time of 50 minutes. Within a certain concentration range, the greater the concentration of the flavonoids extract of pecan shell is, the higher its antioxidant capacity will be.

Key words: pecan shell, total flavonoids, orthogonal design, antioxidant activity

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