Journal of Tianjin Agricultural University ›› 2024, Vol. 31 ›› Issue (1): 36-40.doi: 10.19640/j.cnki.jtau.2024.01.007

• Researches and Scientific Notes • Previous Articles     Next Articles

Screening, identification of strain ZYRH-19 and characteristics of its product welan gum

Zhang Yanga, Lu Heganga, You Pengzhia, Li Xiaoyanb, Huang Haidonga,Corresponding Author   

  1. Tianjin Agricultural University, a. College of Agronomy and Resources Environment, b. College of Food Science and Bioengineering, Tianjin 300392, China
  • Received:2022-04-29 Online:2024-02-29 Published:2024-04-02

Abstract: In this study, a strain ZYRH-19 producing extracellular polysaccharide was isolated from soil. It was classified and identified from the perspectives of morphology, physiology, biochemistry, and 16S rDNA gene sequence, and the extracellular polysaccharide synthesized by strain ZYRH-19 was analyzed and identified. The results showed that ZYRH-19 was a strain of Sphingomonas sp., and its homology with Sphingomonas sp. ATCC 31555 was 99.93%. The extracellular polysaccharide synthesized by strain ZYRH-19 was welan gum with weight average molecular weight of 5.3×106 u. The welan gum solution synthesized by ZYRH-19 had good dilution shear property at room temperature. The viscosity of the product solution could reach 1 444.1 mPa·s at the 6 r/min shear rate, and its gel strength was 22.6 g. It had good temperature resistance at 160 ℃. The results showed that the performance of welan gum synthesized by strain ZYRH-19 is very good, which provides a new strain resource for the industrial production of welan gum.

Key words: Sphingomonas, taxonomic identification, welan gum, polysaccharide

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