响应面法优化藜麦多糖提取工艺条件

doi:10.19640/j.cnki.jtau.2020.04.009

Journal of Tianjin Agricultural University ›› 2020, Vol. 27 ›› Issue (4): 43-48.doi: 10.19640/j.cnki.jtau.2020.04.009

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Study on extraction process of quinoa polysaccharide by response surface methodogy

Guo Yi¹, Yang Xuan¹, Xiao Ping^{1,2,Corresponding Author}

1. College of Food Science and Bioengineering, Tianjin Agricultural University, Tianjin 300392, China;
2. Tianjin Engineering and Technology Research Center of Agricultural Products Processing, Tianjin 300392, China

Received:2019-09-12 Online:2020-12-31 Published:2020-12-25

Abstract

Abstract: In this study, quinoa polysaccharide was extracted by water extraction method, cellulase extraction method and ultrasonic extraction method using the red color quinoa, white color quinoa and black color quinoa as material with the extraction rate of quinoa polysaccharide as an inspection index. The results showed that the highest extraction rate of polysaccharide was 6.8% with white quinoa through the enzyme extraction method, the red quinoa polysaccharide and black quinoa polysaccharide extraction rate were 6.4% and 5.9%, respectively. Furthermore, the single factor and Box-Behnken experiments were employed to optimize the cellulase extraction process for highest yield of white quinoa polysaccharides. The results suggested that the optimal extraction conditions were material-liquid ratio 1∶32 g/mL, the addition of cellulase 2 000 U/g, extraction temperature 65 ℃, and extraction time 90 min. Under the experiment conditions, the yield of polysaccharide was 8.0%.

Key words: quinoa, polysaccharide, cellulase enzyme extraction, Box-Behnken test

CLC Number:

TS201.1

Guo Yi, Yang Xuan, Xiao Ping. Study on extraction process of quinoa polysaccharide by response surface methodogy[J]. Journal of Tianjin Agricultural University, 2020, 27(4): 43-48.

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Study on extraction process of quinoa polysaccharide by response surface methodogy

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