苹果砧木G.11试管苗增殖与生根培养研究

doi:10.19640/j.cnki.jtau.2019.04.008

Journal of Tianjin Agricultural University ›› 2019, Vol. 26 ›› Issue (4): 38-42.doi: 10.19640/j.cnki.jtau.2019.04.008

• Researches and Scientific Notes • Previous Articles Next Articles

Micro shoot proliferation and rooting of apple rootstock G.11

GUO Jing¹, CHAI Ci-jiang^{1, Corresponding Author}, SHI Yan-shan¹, LUO Jian-xia¹, JIANG Wen²

1. College of Horticulture and Landscape, Tianjin Agricultural University, Tianjin 300384, China;
2. Tianjin Cherry Valley Agricultural Technological Development Co. Ltd, Tianjin 301908, China

Received:2019-04-03 Published:2020-01-19

Abstract

Abstract: The stem sections of apple rootstock G.11 plantlet in vitro were respectively inoculated in MS media with different concentrations of 6-BA or KT, or inoculated in WPM media with different concentrations of 6-BA for proliferation. The stem sections were respectively inoculated in soil supporting media for rooting. The results showed that 6-BA promoted G.11 micro stem sections budding and proliferation obviously,while KT had no effect on this. Compared with MS medium, WPM medium reduced vitrification rate of G.11 plantlet in vitro significantly. In WPM medium containing 0.5 mg/L of 6-BA, micro shoot multiplication coefficient of G.11 was 4.9 and vitrification rate was 0. Rooting rate of the G.11 micro shoot cultured in clay soil or sandy soil supporting media were 75.9% and 76.7%, respectively. Moreover, the plantlet had root hair. These results provide basic data for establishing the micro rapid propagation technique of G.11.

Key words: apple rootstock G.11, micro shoot, multiplication, rooting

CLC Number:

GUO Jing, CHAI Ci-jiang, SHI Yan-shan, LUO Jian-xia, JIANG Wen. Micro shoot proliferation and rooting of apple rootstock G.11[J]. Journal of Tianjin Agricultural University, 2019, 26(4): 38-42.

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Micro shoot proliferation and rooting of apple rootstock G.11

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