冰菜多酚的提取优化及抗氧化和抑菌活性研究

doi:10.19640/j.cnki.jtau.2022.03.005

Journal of Tianjin Agricultural University ›› 2022, Vol. 29 ›› Issue (3): 23-28.doi: 10.19640/j.cnki.jtau.2022.03.005

• Researches and Scientific Notes • Previous Articles Next Articles

Study on extraction optimization of polyphenols from Mesembryanthemum crystallinum and its antioxidant and bacteriostatic activities

Wang Zhenli^a, Wang Junbin^{a,b,Corresponding Author}, Wang Min^a, Yang Zongfen^a, Wang Haifeng^c

Tianjin Agricultural University, a. College of Basic Sciences, b. Chemistry Experiment Teaching Center, c. Center for Agricultural Analysis and Measurement, Tianjin 300392, China

Received:2021-05-10 Online:2022-09-30 Published:2022-10-10

Abstract

Abstract: In this study, the extraction method of polyphenols from Mesembryanthemum crystallinum was optimized, and the antioxidant capacity and antibacterial activity of polyphenols from M. crystallinum were determined. The results showed that the optimal extraction conditions were as follows: ethanol concentration was 60%, solid-liquid ratio was 1∶10（g: mL）, extraction temperature was 80 ℃, extraction time was 120 min, under which the extraction rate of polyphenols was 1.42%. The scavenging rates of polyphenols from M. crystallinum to 1,1-diphenyl-2-trinitrophenylhydrazine（DPPH）and hydroxyl radicals increased with the increase of polyphenols concentration. When the concentration of polyphenol extract was 0.10 mg/mL, the scavenging rates of DPPH and hydroxyl radicals were 64.19% and 88.10%, respectively. The inhibitory effect of polyphenol extract on Escherichia coli was obvious. When the extract concentration was 3 mg/mL, the diameter of inhibition zone was 12 mm.

Key words: Mesembryanthemum crystallinum, polyphenol, orthogonal design, antioxidant activity, antibacterial activity

CLC Number:

TS202.3

Wang Zhenli, Wang Junbin, Wang Min, Yang Zongfen, Wang Haifeng. Study on extraction optimization of polyphenols from Mesembryanthemum crystallinum and its antioxidant and bacteriostatic activities[J]. Journal of Tianjin Agricultural University, 2022, 29(3): 23-28.

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: http://xuebao.tjau.edu.cn/EN/10.19640/j.cnki.jtau.2022.03.005

http://xuebao.tjau.edu.cn/EN/Y2022/V29/I3/23

References

[1] 刘慧颖,韩玉燕,蒋润枝. NaCl对冰菜生长发育及重要品质的影响[J]. 江苏农业科学,2019,47(15):184-188.
[2] KIM Y J,KIM H M,KIM H M,et al.Growth and phytochemicals of ice plant(Mesembryanthemum crystallinum L.)as affected by various combined ratios of red and blue LEDs in a closed-type plant production system[J]. Journal of Applied Research on Medicinal and Aromatic Plants,2021,20:100267.
[3] 舒悦,李婷婷,曾艳波. 冰菜的化学成分研究[J]. 天然产物研究与开发,2020,32(10):1704-1708.
[4] 姜楠,王蒙,韦迪哲,等. 植物多酚类物质研究进展[J]. 食品安全质量检测学报,2016,7(2):439-444.
[5] 张佳欣. 多酚类化合物协同抗氧化性研究进展[J]. 现代农业研究,2020,49(1):117-118,121.
[6] 韩露. 中药酚性抗氧化剂清除DPPH自由基机理的推断性研究[D]. 广州:广州中医药大学,2015.
[7] 孙悦,左丽丽. 植物多酚功能的研究进展[J]. 吉林医药学院学报,2020,41(5):386-388.
[8] IBTISSEM B,ABDELLY C,SFAR S.Antioxidant and antibacterial properties of Mesembryanthemum crystallinum and Carpobrotus edulis extracts[J]. Advances in Chemical Engineering and Science,2012,2(3):359-365.
[9] SINGLETON V L,ORTHOFER R M,RAMUELARA- VENTIOS R M. Analysis of total phenols and other oxidation substrates and antioxidants by means of folin- ciocalteu reagent[J]. Methods in Enzymology,1999,299:152-178.
[10] 马骁,王飞飞,袁永雷. 正交试验优选马齿苋黄酮脱色工艺的研究[J]. 食品研究与开发,2018,39(9):88-91.
[11] 常姗姗. 盐生马齿苋主要营养成分分析和提取物的抗氧化活性及抑菌作用的研究[D]. 南京:南京师范大学,2013.
[12] 康坤,王俊斌,王海凤,等. 碧根果壳总黄酮的提取工艺优化和抗氧化活性研究[J]. 天津农学院学报,2020,27(3):74-78.
[13] 杜丽娟,苏秀芳,梁翠君. 樱花叶总黄酮的超声波法提取工艺优化及其抗氧化能力的研究[J]. 食品工业科技,2020,41(11):221-225,233.
[14] SMIRONFF N,CUMBES Q J.Hyroxyl radical scavenging activity of compatible solutes[J]. Phytochemistry,1989,284(4):1057-1060.
[15] 尚红梅,郭玮,潘丹,等. 干燥方式对菊苣根多酚含量和抗氧化活性的影响[J]. 食品科学,2015,36(1):84-88.
[16] 忻晓庭,刘大群,郑美瑜. 热风干燥温度对冰菜干燥动力学、多酚含量及抗氧化活性的影响[J]. 中国食品学报,2020,20(11):148-156.
[17] 曹小燕,杨海涛. 响应面法优化超声辅助提取荠菜多酚工艺及其抗氧活性研究[J]. 食品工业科技,2019,40(2):223-228,232.
[18] 蒋蕾. 莲子皮渣中酚类物质及其抗氧化活性研究[D]. 杭州:浙江大学,2019.
[19] PRIOR R L,CAO G J.Analysis of botanicals and dietary supplements for antioxidant capacity:a review[J]. J AOAC Int,2000,83(4):950-956.
[20] IBTISSEM B,ABDELLY C,SFAR S.Antioxidant and antibacterial properties of Mesembryanthemum crystallinum and Carpobrotus edulis extracts[J]. Advances in Chemical Engineering and Science,2012,2(3):359-365.
[21] SEUNGMI K,SEONJEONG K,SUHYUN H.Biochemical components and physiological activities of ice plant (Mesembryanthemum crystallinum)[J]. J Korean Soc Food Sci Nutr,2016,45(12):1732-1739.
[22] 张笑,李颖畅. 植物多酚的抑菌活性及其在食品保鲜中的应用[J]. 食品安全质量检测学报,2013,4(3):769-773.

Study on extraction optimization of polyphenols from Mesembryanthemum crystallinum and its antioxidant and bacteriostatic activities

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 11

Recommended Articles

Metrics

Comments

[1]	Zhang Huan, Zhang Pingping, Li Xiang, Tong Xiaohui, Wang Feng, Zhao Chenchen. Effect of enzymatic hydrolysis on soluble dietary fiber and antioxidant activity in old asparagus stalks [J]. Journal of Tianjin Agricultural University, 2023, 30(6): 11-15.
[2]	Yang Zongfen, Wang Min, Wang Zhenli, Wang Haifeng, Wang Junbin. Study on antioxidant and antibacterial activity of polysaccharide extract in Mesembryanthemum crystallinum [J]. Journal of Tianjin Agricultural University, 2023, 30(1): 1-5.
[3]	Dong Xinyi, Chen Huihui, ZhangYuwei, Wang Yingchao, Zhang Guixia, Wang Ruixi. Purification of polyphenols from Aronia melanocarpa by macroporous adsorption resin [J]. Journal of Tianjin Agricultural University, 2022, 29(1): 19-23.
[4]	Wang Min, Wang Junbin, Wang Zhenli, Yang Zongfen, Wang Haifeng. Study on the optimization of extraction and the antioxidant activity of total flavonoids in Mesembryanthemum crystallinum [J]. Journal of Tianjin Agricultural University, 2022, 29(1): 33-36.
[5]	Xiong Qian, Liu Liyao, Wang Qingkui, Liu Lin, Liu Yue. Emulsion formulation optimization of Cinnamon oil [J]. Journal of Tianjin Agricultural University, 2021, 28(1): 40-44.
[6]	LI Shu-he, WANG Ning, ZHAO Qian, GUO Ying. Effects of drought stress on the growth of Mesembryanthemum Crystallinum L. [J]. Journal of Tianjin Agricultural University, 2020, 27(3): 35-38.
[7]	KANG Kun, WANG Jun-bin, WANG Hai-feng, WANG Feng-tang, WANG Yao-guo, WANG Hao. Optimization of extraction and antioxidant activity of total flavonoids in pecan shell [J]. Journal of Tianjin Agricultural University, 2020, 27(3): 74-78.
[8]	CHENG Bin, LI Xiao-yan, PAN Yi-chen, FENG Xue, HUANG Hai-dong. Screening, identification of sanxan-degrading microorganisms and antioxidant activities of degradation product [J]. Journal of Tianjin Agricultural University, 2019, 26(4): 43-48.
[9]	LIU Pei, WANG Jian-hui, WU Jia-li, XING Fang, HE Xin-yi, HUANG Zong-hai. Changes in Antioxidant Activity of Lotus Root Chips during Combination Drying [J]. Journal of Tianjin Agricultural University, 2016, 23(3): 6-10.
[10]	YU Wei-wei, WANG Hai-wei, LI Hui, FENG Tao, YAN Guo-rong. Effects of Culture Conditions on Pollen Germination and Growth of Zaosu Pear in Vitro [J]. Journal of Tianjin Agricultural University, 2014, 21(4): 29-32.
[11]	ZHU Hua-ling, BAN Li-tong, SHI Jun, BU Lu-xia, WEI Zhen. Reactivity Prediction of Benzoyl Acetone and Synthesis, Characterization, Antibacterial Activities of Its Metal Complexes [J]. Journal of Tianjin Agricultural University, 2012, 19(4): 18-23.