5种常见致病菌菌液OD值与其活菌数相关性研究

Journal of Tianjin Agricultural University ›› 2017, Vol. 24 ›› Issue (4): 42-45.

• Researches and Scientific Notes • Previous Articles Next Articles

Study on Correlation between OD Values and Viable Count of Five Common Pathogenic Bacteria

LIN Jing, HE Jia-xi, SHANG Cui-ling, MENG Jia-li, LI Xin, JIN Tian-ming^{Corresponding Author}

College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin 300384, China

Received:2017-05-20 Online:2017-12-31 Published:2019-10-15

Abstract

Abstract: The purpose of this test is to determine E. coli, Pasteurella, Staphylococcus aureus, Staphylococcus epidermis and Streptococcus bovis OD₆₀₀values, and the relationship with the number of living bacteria. First of all, the bacteria were measured by turbidimetric method in 1.5, 3, 4, 6, 8, 10, 12, 14, 16, 18 h OD₆₀₀ value of the bacteria, and dilution bacteria liquid for the plate count. The relationship between the OD₆₀₀ and the bacterial concentration was obtained, in logarithmic phase of bacterial growth, E. coli: y=8 708.7e^36.43^x（R²=0.828）; Pasteurella: y=34.82e^35.57^x（R²=0.866）; Staphylococcus aureus: y=49 331e^28.16^x（R²=0.743）; Staphylococcus epidermis: y=4×10⁸e^24.73^x（R²=0.928）; Streptococcus bovis: y=8×10⁹e^68.21^x（R²=0.971）, which provide the basis for measuring the number of live bacteria in the bacterial fluid.

Key words: OD₆₀₀ value of bacterial fluid, plate counting, quantitative relationship

CLC Number:

S852.61

LIN Jing, HE Jia-xi, SHANG Cui-ling, MENG Jia-li, LI Xin, JIN Tian-ming. Study on Correlation between OD Values and Viable Count of Five Common Pathogenic Bacteria[J]. Journal of Tianjin Agricultural University, 2017, 24(4): 42-45.

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Study on Correlation between OD Values and Viable Count of Five Common Pathogenic Bacteria

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