犬源伪中间型葡萄球菌的分离鉴定及药敏特性

doi:10.19640/j.cnki.jtau.2021.03.009

Journal of Tianjin Agricultural University ›› 2021, Vol. 28 ›› Issue (3): 42-45.doi: 10.19640/j.cnki.jtau.2021.03.009

• Researches and Scientific Notes • Previous Articles Next Articles

Isolation, identification and drug resistance of canine Staphylococcus pseudointermediate

Ding Qiaoling, Liu Shuyue, Meng Ze, Li Jianjun^{Corresponding Author}

Tianjin Key Laboratory of Agricultural Animal Breeding and Healthy Husbandry, College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin 300392, China

Received:2020-04-05 Online:2021-09-30 Published:2021-10-07

Abstract

Abstract: An 11-year-old golden-haired dog with pyoderma was admitted to a pet hospital in Tianjin. The secretions of eyes, nose and skin were collected for bacterial isolation and culture. The results of culture characteristics, staining microscopy, biochemical test, plasma coagulase test, drug susceptibility test and 16S rDNA sequence analysis showed that the isolate TJ-1 decomposed fructose, lactose, mushroom sugar, sucrose, mannose, maltose, mannitol and nitrate reduction were negative, while plasma coagulase test was positive. The 16S rDNA sequence was amplified by PCR and sequenced. The phylogenetic tree analysis showed that the isolate was clustered with Staphylococcus pseudintermedius and TJ-1 strain was identified as S. pseudintermedius. Drug susceptibility test showed that the bacteria were susceptible to oxacillin, ceftazidime, cefoperazone, cefalexin, doxycycline, cefazolin, minocycline, cefradine, cefuroxime and amikacin, but resistant to erythromycin, penicillin, ampicillin and carboxybenicillin.

Key words: dog, Staphylococcus pseudointermediate, isolation and identification, antibiotics sensitivity test

CLC Number:

S852.61

Ding Qiaoling, Liu Shuyue, Meng Ze, Li Jianjun. Isolation, identification and drug resistance of canine Staphylococcus pseudointermediate[J]. Journal of Tianjin Agricultural University, 2021, 28(3): 42-45.

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Isolation, identification and drug resistance of canine Staphylococcus pseudointermediate

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