犬链球菌M蛋白基因的克隆及生物信息学分析

doi:10.19640/j.cnki.jtau.2018.01.010

Journal of Tianjin Agricultural University ›› 2018, Vol. 25 ›› Issue (1): 44-48.doi: 10.19640/j.cnki.jtau.2018.01.010

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Cloning and bioinformatics analysis of M gene in Streptococcus canis

LIANG Yu, LIU Yan-fei, SUN Xiao-li, YANG Jian-de^{Corresponding Author}

College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin 300384, China

Received:2017-04-10 Online:2018-03-20 Published:2019-11-05

Abstract

Abstract: In order to provide the reference for developing epitope vaccine and diagnosis of Streptococcus, M protein of Streptococcus canis（SCM）gene was cloned and analyzed by bioinformatics software to predict its protein structure and function in this study. SCM was a stable hydrophilic protein and transmembrane protein proved by analysis of ExPASy ProtParam on-line software. This protein was predicted to contain signal peptide by SignalP 4.1 software. Five separate methods of SOPMA showed that SCM had α helix as the main component of secondary structure, accounted for 83.53%. The three-dimensional structure of SCM determined by using SWISS-MODEL work-space was predicted to have the same result with the secondary structure. By using Protean modules of DNAStar software, SCM hydrophilicity plot, secondary structure, antigenicity index, surface probability plot and flexible regions are analyzed and compared to find that B cell epitopes were predicted at the region No. 55~59 and No.377~379.

Key words: SCM, bioinformation analysis, protein structure, B cell epitope

CLC Number:

S852.61

LIANG Yu, LIU Yan-fei, SUN Xiao-li, YANG Jian-de. Cloning and bioinformatics analysis of M gene in Streptococcus canis[J]. Journal of Tianjin Agricultural University, 2018, 25(1): 44-48.

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References

[1] 刘燕霏,杨建德. 犬链球菌重组M蛋白的免疫学分析[J]. 黑龙江畜牧兽医,2013(7):87-89.
[2] Fulde M,Rohde M,Polok A,et al.Cooperative plasminogen recruitment to the surface of Streptococcus canis via M protein and enolase enhances bacterial survival[J]. M Bio,2013,4(2):629-641.
[3] Steer A C,Carapetis J R,Dale J B,et al.Status of research and development of vaccines for Streptococcus pyogenes[J].Vaccine,2016,34(26):2953-2958.
[4] 杨建德,刘燕霏,徐军,等. 犬链球菌保护性抗原的鉴定[J]. 中国预防兽医学报,2009,31(9):680-683.
[5] Kuo C F,Tsao N,Hsieh I C,et al.Immunization with a streptococcal multiple-epitope recombinant protein protects mice against invasive group A streptococcal infection[J]. Plos One,2017,12(3):e0174464.
[7] 颜其贵,雷燕,张志和,等. 大熊猫轮状病毒CH-l株外衣壳蛋白(VP7)基因的克隆和生物信息学分析[J].畜牧兽医学报,2010,41(6):705-710.
[8] 冯旭芳,张双翔,周碧君,等. 猪轮状病毒贵州株VP7基因克隆及生物信息学分析[J]. 畜牧与兽医,2016,48(4):87-93.
[9] 胡晓维,靳聪飞,刘新峰,等. 牛ACOT11基因克隆及其编码蛋白生物信息学分析[J]. 天津农学院学报,2015,22(3):1-6.
[10] Thigpen M C,Whitney C G,Messonnier N E,et a1. Bacterial meningitis in the United States[J].N Engl J med,2011,364(21):2016-2025.
[11] 朱晓琳,王劭,陈少莺,等. 猪传染性胃肠炎病毒福建株M基因的克隆与生物信息学分析[J]. 福建农业学报,2011,26(5):925-929.
[12] Converso T R,Goulart C,Rodriguez D,et al.Systemic immunization with rPotD reduces Streptococcus pneumoniae nasopharyngeal colonization in mice[J]. Vaccine,2017, 35(1):149-155.
[13] Williamson D A,Smeesters P R,Steer A C,et al.Comparative M-protein analysis of Streptococcus pyogenes frompharyngitis and skin infections in New Zealand:Implications for vaccine development[J]. BMC Infect Dis,2016,16(1):561.
[14] 许宇彪,杨建荣,李碧锦. 人类miR-425靶基因预测及生物信息学特征分析[J]. 河北医药,2017,39(7):977-980.
[15] Cornick J E,Özlem Tastan Bishop,Yalcin F,et al.The global distribution and diversity of protein vaccine candidate antigens in the highly virulent Streptococcus pnuemoniae serotype 1[J]. Vaccine,2017,35(6):972-980.

Cloning and bioinformatics analysis of M gene in Streptococcus canis

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