Abstract: With the development of the urban pet industry, suppurative diseases of companion animals are increasing day by day.To explore its associated pathogens, this study was performed on etilolgical identification from a case of a canine uterus with pus. Bacteriological detection method was used to isolate and purify the collected disease materials, such as smear staining, microscopic examination and biochemical identification. Then, 16S rRNA molecular sequence analysis was used to identify the the isolated strain. It was found that the morphological features of the isolated strain and the biochemical results were consistent with the Enterococcus faecalis. The 16S rRNA of the isolated strain was also as high as 99% homologous with the reference stains. Drug susceptibility test showed that the isolate was resistant to tetracycline, penicillin G, minocycline, erythromycin, ciprofloxacin, norfloxacin, vancomycin, levofloxacin, clindamycin, polymyxin B, chloramphenicol, compound sulfamethoxazole, and clarithromycin, and only sensitive to oxacillin and nitrofurantoin. These results provided a reference for the treatment of this type of multidrug-resistant enterococcal infection.

Key words: Enterococcus faecalis, biochemical identification, evolutionary tree analysis, drug sensitive test