枣树根部内生细菌的分离及对3种枣病原真菌拮抗活性研究

doi:10.19640/j.cnki.jtau.2019.03.003

Journal of Tianjin Agricultural University ›› 2019, Vol. 26 ›› Issue (3): 12-16.doi: 10.19640/j.cnki.jtau.2019.03.003

• Researches and Scientific Notes • Previous Articles Next Articles

Isolation and characterization of endophytic bacteria from jujube roots and their antimicrobial activities to three kinds of jujube pathogenic fungi

QIU Zhi-wei, CHEN You-zhong, YU Ao-an, ZHU Wei-yao, YANG Jun-lin, CHEN Zhao-rong

College of Horticulture and Landscape, Tianjin Agricultural University, Tianjin 300384, China

Received:2019-05-10 Online:2019-09-30 Published:2019-09-30

Abstract

Abstract: Jujube is an important economic fruit tree widely planted in Tianjin. Endophytic bacteria is a new biological resource in plant disease control, which has broad theoretical research significance and development prospect. In this study, 18 strains of endogenous bacteria, named as z1-z18 were isolated from fresh jujube root tissues after disinfection, and were stained with gram and sequenced with 16S rRNA. A total of 6 genera including Chryseobacterium, Pseudomonas, Bacillus Cohn, Shigella Castellani, Escherichia coli and Stenotrophomonas were identified. A strain Z18, which had antagonistic effects on the pathogens of jujube black spots, jujube chains and jujube wheelies, was screened out by the plate confrontation test. After identification, it was preliminarily identified as bacillus SPP., and the gram stain was positive. The results can provide materials for biological control of endophytic bacteria in jujube.

Key words: endophytic bacteria, isolation, antagonism, biocontrol bacterium

CLC Number:

S431.192

QIU Zhi-wei, CHEN You-zhong, YU Ao-an, ZHU Wei-yao, YANG Jun-lin, CHEN Zhao-rong. Isolation and characterization of endophytic bacteria from jujube roots and their antimicrobial activities to three kinds of jujube pathogenic fungi[J]. Journal of Tianjin Agricultural University, 2019, 26(3): 12-16.

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Isolation and characterization of endophytic bacteria from jujube roots and their antimicrobial activities to three kinds of jujube pathogenic fungi

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