猪胸膜肺炎放线杆菌的分离鉴定及药敏性试验

doi:10.19640/j.cnki.jtau.2018.04.008

Journal of Tianjin Agricultural University ›› 2018, Vol. 25 ›› Issue (4): 33-37.doi: 10.19640/j.cnki.jtau.2018.04.008

• Researches and Scientific Notes • Previous Articles Next Articles

The isolation and identification of pathogen and drug susceptibility test for Actinobacillus pleuropneumoniae

WANG Yu-hui, LI Xin, SUN Xiao-li, MENG Jia-li, SHANG Cui-ling, LIN Jing, JIN Tian-ming^{Corresponding Author}

College of Animal Science and Veterinary Medicine,Tianjin Agricultural University, Tianjin 300384, China

Received:2018-06-06 Online:2018-12-20 Published:2019-11-12

Abstract

Abstract: ：In this study, three young pigs in a large pig farm from Tianjin were selected as the research objects. The results of the pathological changes of the organs, the isolation and identification of morphology, the culture medium, the biochemistry, 16S rRNA amplification and PCR were carried out to identify pathogenic bacteria. The bacteria were identified as pathogenic bacteria by animal pathogenicity test. The results showed that the cause of swine’s death was Actinobacillus pleuropneumoniae. Then drug sensitivity test showed that APP was sensitive to cefoperazone; moderately sensitive to chloramphenicol, amoxicillin and ciprofloxacin; and resistantto doxycycline, streptomycin, kanamycin, penicillin, tetracycline, ampicillin, vancomycin, and gentamicin. This experiment provides a theoretical basis for the clinical diagnosis and medication of APP.

Key words: Actinobacillus pleuropneumoniae, 16S rRNA, isolation and identification, drug sensitivity test

CLC Number:

WANG Yu-hui, LI Xin, SUN Xiao-li, MENG Jia-li, SHANG Cui-ling, LIN Jing, JIN Tian-ming. The isolation and identification of pathogen and drug susceptibility test for Actinobacillus pleuropneumoniae[J]. Journal of Tianjin Agricultural University, 2018, 25(4): 33-37.

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The isolation and identification of pathogen and drug susceptibility test for Actinobacillus pleuropneumoniae

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