不同溶血性大肠杆菌的分离与鉴定

doi:10.19640/j.cnki.jtau.2020.01.014

Journal of Tianjin Agricultural University ›› 2020, Vol. 27 ›› Issue (1): 61-64.doi: 10.19640/j.cnki.jtau.2020.01.014

• Researches and Scientific Notes • Previous Articles Next Articles

Isolation and identification of two hemolytic strains of canine Escherichia coli

DU Rong-qi, Ren Jun, QIAO Qi, ZHANG Qing, LIU Yan-fei^{Corresponding Author}, YANG Jian-de^{Corresponding Author}

College of Animal Science and Veterinary Medicine, Tianjin Agricultural University , Tianjin 300384, China

Received:2019-04-25 Published:2020-04-01

Abstract

Abstract: In order to clarify the pathogenic causes of purulent diseases of companion animals, the pathogenic microbiological identification of uterine purulent secretions of two female dogs in an outpatient department of an animal hospital was carried out. Colony morphology, gram staining microscopy, biochemical test, drug susceptibility test and 16S rRNA gene sequence alignment analysis of the inoculated medium were observed. The isolates were alpha hemolytic and beta hemolytic Escherichia coli, respectively. Beta hemolytic Escherichia coli was resistant to kanamycin, streptomycin and cefothiophene. Alpha hemolytic Escherichia coli was only resistant to medilamycin. The resistance of beta hemolytic strain is significantly higher than that of alpha hemolytic strain, which provides a reference for the prevention and treatment of related infections caused by hemolytic Escherichia coli.

Key words: hemolytic Escherichia coli, 16S rRNA, isolation and identification, hemolysin

CLC Number:

S858.611

DU Rong-qi, Ren Jun, QIAO Qi, ZHANG Qing, LIU Yan-fei, YANG Jian-de. Isolation and identification of two hemolytic strains of canine Escherichia coli[J]. Journal of Tianjin Agricultural University, 2020, 27(1): 61-64.

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Isolation and identification of two hemolytic strains of canine Escherichia coli

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