菲律宾蛤仔中一株枯草芽胞杆菌的分离鉴定及生物学特征研究

doi:10.19640/j.cnki.jtau.2018.02.011

Journal of Tianjin Agricultural University ›› 2018, Vol. 25 ›› Issue (2): 42-46.doi: 10.19640/j.cnki.jtau.2018.02.011

• Researches and Scientific Notes • Previous Articles Next Articles

Isolation, identification and biological characteristics of a Bacillus subtilis from Ruditapes philippinarum

MAO Qing¹, CHEN Feng¹, LIANG Jian¹, LIU Jun-feng², LÜ Ai-jun¹, HU Xiu-cai¹, GUO Yong-jun¹, XING Ke-zhi¹, SUN Jing-feng^{1,Corresponding Author}

1.Tianjin Key Lab of Aqua-Ecology and Aquaculture, College of Fisheries, Tianjin Agricultural University, Tianjin 300384, China;
2. Tianjin Yushengtang Biotechnology Co. Ltd., Tianjin 300404, China

Received:2018-01-04 Online:2018-06-20 Published:2019-11-12

Abstract

Abstract: A bacterium strain was isolated and purified from the hepatopancreas of healthy Ruditapes philippinarum and named G6. This bacterium strain was identified as Bacillus subtilis according to the results of morphological observation, physiological and biochemical characteristics, 16S rDNA sequence, and phylogenetic analysis. Its biological characteristics were studied, including growth curve, resistance to high temperature, acid, and bile salt. Results showed that the strain G6 had a long growth period and a stationary phase from 26 to 34 h and was resistant to high temperature, acid, and bile salt. This report provided a scientific base for application of G6 as the probiotic for aquatic animals.

Key words: Ruditapes philippinarum, Bacillus subtilis, isolation, identification, growth characteristics, resistance

CLC Number:

S917.4

MAO Qing, CHEN Feng, LIANG Jian, LIU Jun-feng, LÜ Ai-jun, HU Xiu-cai, GUO Yong-jun, XING Ke-zhi, SUN Jing-feng. Isolation, identification and biological characteristics of a Bacillus subtilis from Ruditapes philippinarum[J]. Journal of Tianjin Agricultural University, 2018, 25(2): 42-46.

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Isolation, identification and biological characteristics of a Bacillus subtilis from Ruditapes philippinarum

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