猪胰脂肪酶在麸皮上的固定化及催化活性

doi:10.19640/j.cnki.jtau.2024.06.012

Journal of Tianjin Agricultural University ›› 2024, Vol. 31 ›› Issue (6): 75-78.doi: 10.19640/j.cnki.jtau.2024.06.012

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Immobilization of porcine pancreas lipase carried on wheat bran and therefore catalytic activity

Chen Lu^a, Cong Fangdi^{a,Corresponding Author}, Ma Liya^a, Wang Yingchao^a, Zhang Dajuan^b, Feng Ziran^a

Tianjin Agricultural University, a. College of Basic Sciences, b. Tianjin Key Lab of Aqua-Ecology and Aquaculture, College of Fisheries, Tianjin 300392, China

Received:2024-02-26 Online:2024-12-31 Published:2024-12-31

Abstract

Abstract: To enhance the catalytic ability of porcine pancreas lipase, it was immobilized on wheat bran by physical adsorption in a column glass bottle at 37 ℃ and 160 r/min, and used to catalyze the non-aqueous transesterification between hexanol and vinyl acetate as well as the hydrolysis of peanut oil at 37 ℃ and 160 r/min. It was found that the suitable mass ratio of wheat bran and lipase was determined to be 20:10（mg:mg）. At this ratio, the immobilized lipase behaved the highest non-aqueous activity, being more than10-fold activity of 10 mg native lipase. The activity decreased less than 0.2% after the immobilized lipase was reused 5 times in the organic system, each time for 8 h, which showed that it had a good catalytic stability. In hydrolysis of peanut oil, the immobilized lipase could hydrolyze peanut oil 4.7 folds of that accomplished by the native lipase with equal mass, which means that it has potential as a feed additive to assist animal digestion.

Key words: lipase, catalysis, activity, immobilization, wheat bran

CLC Number:

Q814

Chen Lu, Cong Fangdi, Ma Liya, Wang Yingchao, Zhang Dajuan, Feng Ziran. Immobilization of porcine pancreas lipase carried on wheat bran and therefore catalytic activity[J]. Journal of Tianjin Agricultural University, 2024, 31(6): 75-78.

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Immobilization of porcine pancreas lipase carried on wheat bran and therefore catalytic activity

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