干、鲜银耳粗多糖提取工艺优化及抗氧化活性研究

doi:10.19640/j.cnki.jtau.2024.06.011

Journal of Tianjin Agricultural University ›› 2024, Vol. 31 ›› Issue (6): 64-74.doi: 10.19640/j.cnki.jtau.2024.06.011

• Researches and Scientific Notes • Previous Articles Next Articles

Optimization of extraction process and antioxidant activity of crude polysaccharide from dried and fresh Tremella Fuciformis

Dai Hanzhe, Dong Xinyi, Zhang Wenxin, Lu Zikang, Zhang Pingping^{Corresponding Author}

College of Food Science and Biotechnology, Tianjin Agricultural University, Tianjin 300392, China

Received:2024-04-01 Online:2024-12-31 Published:2024-12-31

Abstract

Abstract: The crude polysaccharides of Tremella fuciformis were extracted from dried and fresh Tremella fuciformis, and the extraction technology and antioxidant activity were compared by single factor and response surface tests. The results showed that the optimal extraction conditions of crude polysaccharide from dried tremella fuciformis were as follows: ratio of solid to liquid 1:49（g:mL）, extraction temperature 71 ℃, extraction time 3 h, and ultrasonic time 40 min. The extraction rate of crude polysaccharide was（20.07±0.43）%; the optimal extraction conditions of crude polysaccharide from fresh Tremella fuciformis were as follows: ratio of solid to liquid 1:32（g: mL）, extraction temperature 71 ℃, extraction time 4 h, ultrasonic time 40 min, and extraction rate of crude polysaccharide was（44.13±0.79）%. The extraction rate of crude polysaccharide from dried Tremella fuciformis was only 45.48% of that from fresh Tremella fuciformis. The superoxide anion and hydroxyl radical scavenging rates of crude polysaccharides from dried and fresh Tremella fuciformis were measured. The IC₅₀ values were 5.12, 4.01, 3.59, and 3.63 mg/mL, respectively. At the mass concentration of 5 mg/mL, the FRAP values of crude polysaccharides from dried and fresh Tremella fuciformis were 0.36 and 0.51, respectively. The antioxidant activity of the two crude polysaccharides increased with the increase of mass concentration, and the antioxidant activity of crude polysaccharides from fresh Tremella fuciformis was higher than that from dried Tremella fuciformis.

Key words: Tremella fuciformis, polysaccharides, extraction, antioxidant activity

CLC Number:

TS201.1

Dai Hanzhe, Dong Xinyi, Zhang Wenxin, Lu Zikang, Zhang Pingping. Optimization of extraction process and antioxidant activity of crude polysaccharide from dried and fresh Tremella Fuciformis[J]. Journal of Tianjin Agricultural University, 2024, 31(6): 64-74.

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Optimization of extraction process and antioxidant activity of crude polysaccharide from dried and fresh Tremella Fuciformis

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