盐胁迫下耐盐苜蓿松散型愈伤组织生理生化特性变化

Journal of Tianjin Agricultural University ›› 2016, Vol. 23 ›› Issue (4): 38-42.

• Researches and Scientific Notes • Previous Articles Next Articles

The Physiological and Biochemical Characteristics Changes of Loose Salt-tolerant Callus of Alfalfa under Salt Stress

YANG Jing-hui¹, WU Zhi-yu¹, LIU Yan-jun¹, QIN Yan-jun¹, LI Bing², HUANG Wei-zi¹

1. College of Horticulture and Landscape, Tianjin Agricultural University, Tianjin 300384, China;
2. College of Business Administration, Tianshi College, Tianjin 301700, China

Received:2016-03-22 Online:2016-12-20 Published:2019-10-14

Abstract

Abstract: In order to understand the physiological and biochemical changes of alfalfa salt resistant mutant, the content of soluble sugar, malondialdehyde, soluble protein, free proline and the cell membrane permeability, ionic concentration and activity of SOD & POD enzyme were studied with loose salt-tolerant and ordinary callus of alfalfa and under 2% salt stress. The results showed that the soluble sugar, POD enzyme activity, free proline and K⁺/Na⁺ of loose salt-tolerant callus was respectively 0.757, 0.266, 6.525 and 0.700 times higher than control group. However, the SOD enzyme activity, malondialdehyde, cell membrane permeability, and Na⁺ & K⁺ was 1.807, 0.878, 0.553, 2.903 and 1.296 times lower than control group. In conclusion, Loose salt-tolerant callus increased in soluble sugar, POD activity, free proline and the ratio of K⁺/Na⁺ under salt stress, while decreased in malondialdehyde cell membrane permeability and Na⁺, furthermore there was no significant change in soluble protein. In short , alfalfa mutations salt-tolerant callus have more resistance.

Key words: alfalfa, loosely, callus, salt stress, physiological and biochemical

CLC Number:

S541.9

YANG Jing-hui, WU Zhi-yu, LIU Yan-jun, QIN Yan-jun, LI Bing, HUANG Wei-zi. The Physiological and Biochemical Characteristics Changes of Loose Salt-tolerant Callus of Alfalfa under Salt Stress[J]. Journal of Tianjin Agricultural University, 2016, 23(4): 38-42.

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References

[1] 李波,徐婉玉,彭丹,等. 卫星搭载苜蓿种子叶片愈伤组织对逆境胁迫的响应[J]. 干旱地区农业研究,2015,33(8):167-171.
[2] 田瑞娟,刘艳军,杨静慧,等. 不同激素不同外植体对苜蓿品种再生的影响[J]. 南方农业,2007,1(3):13-14.
[3] 杜长城,杨静慧,任慧朝,等. 不同品种紫花苜蓿的耐盐性筛选试验[J]. 天津农业科学,2008,14(5):14-16.
[4] 田瑞娟,杨静慧,梁国鲁,等. 不同品种紫花苜蓿耐盐性研究[J]. 西南农业大学学报,2006,28(6):933-936.
[5] 张学云. 紫花苜蓿耐盐材料的鉴定及胚状体耐盐性诱变研究[D]. 北京:中国农业科学院,2013.
[6] Hu Yanyan,Yang Jinghui, Zhang Chao,et al.Difference of Cell Morphology on Different Callus Types of Alfalfa[J]. Applied Mechanics and Materials,2015,707:137-143.
[7] 杨静慧,秦艳筠,刘婷,等. 两种愈伤组织生理生化特性和形态结构比较[J]. 天津农业科学,2014,20(8):22-24.
[8] 苗博瑛,刘艳军,杨静慧. 黑莓松散型胚性愈伤组织的诱导[J]. 山西农业科学,2014,42(3):209-212,216.
[9] Qin Yanjun,Gong Wuque,Liu Ting,et al.Difference of morphological structure and characteristics of physiology andbiochemistry between two types of alfalfa callus,(ICEMEE 2014)[J]. Applied Mechanics and Materials,2015,700:306-309.
[10] 刘艳军,张超,杨静慧,等. 塔杨松散型均质胚性愈伤组织培养体系[J]. 植物研究,2016,36(1):123-128.
[11] 薄鹏飞,孙秀玲,孙同虎,等. NaCl胁迫对海滨木槿抗氧化系统和渗透调节的影响[J]. 西北植物学报,2008,28(1):113-118.
[12] 史如霞. NaCl胁迫对唐古特白刺愈伤组织生理生化特性的影响[D]. 兰州:西北师范大学,2010.
[13] Benavides M P,Marconi P L,Gallego S M,et al.relationship between antioxidant defense systems and salt tolerance in solanum tuberosum[J]. Plant Physiology,2000,27:273-278.
[14] 孔凡岩. 百喜草体细胞耐盐突变体筛选及其生理生化特性研究[D]. 合肥:安徽农业大学,2010.
[15] 刘强. 五种木本植物对盐碱胁迫的生理响应及外源物质的作用[D]. 哈尔滨:东北林业大学,2009.
[16] 许鹏. 新疆荒漠区草地与水盐植物系统及优化生态模式[M]. 北京:科学出版社,1998.
[17] 朱建峰,田增荣,余玲,等. 小麦耐盐性基因型差异研究[J]. 甘肃农业科技,1996(8):7-8.
[18] 曹翠玲,李生秀. 氮素形态对玉米幼苗碳水化合物及养分积累的影响[J]. 华中农业大学学报,2003,5(22):457-461.
[19] 葛春辉. 亚麻胚性愈伤的诱导及耐盐愈伤的生理生化特性[D]. 乌鲁木齐:新疆大学,2007.
[20] 韦小敏. 玉米胚性愈伤组织耐盐变异体的筛选以及耐盐性分析[D]. 郑州:河南农业大学,2001.
[21] 陈少瑜,郎南军,贾利强,等. 干旱胁迫对坡柳等抗旱树种幼苗膜脂过氧化及保护酶活性的影响[J]. 植物研究,2006,26(1):88-92.
[22] 陈少瑜,郎南军,贾利强,等. 干旱胁迫对坡柳等抗旱树种幼苗膜脂过氧化及保护酶活性的影响[J]. 植物研究,2006,26(1):88-92.
[23] 武香. 盐胁迫下植物的渗透调节及其适应性研究[D]. 北京:中国林业科学研究院,2012.
[24] 蔡翔君,关诗扬,过晓明. 盐胁迫对3中甘薯品种抗氧化酶及可溶性蛋白的影响[J]. 安徽农业科学,2012,40(17):9228-9229.
[25] Ashraf M.Organic substances responsible for salt tolerance in Eruca sativa[J]. Biologia Plantarum,1994,36(2):255-259.
[26] Ashraf M,Fatima H.Responses of some salt tolerant and salt sensitive lines of safflower(Carthamus tinctorius L.)[J]. Acta Physiol Plant,1995,17:61-71.

The Physiological and Biochemical Characteristics Changes of Loose Salt-tolerant Callus of Alfalfa under Salt Stress

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