草鱼致病性腐败希瓦氏菌的分离鉴定及药敏特性研究

doi:10.19640/j.cnki.jtau.2018.04.012

Journal of Tianjin Agricultural University ›› 2018, Vol. 25 ›› Issue (4): 52-57.doi: 10.19640/j.cnki.jtau.2018.04.012

• Researches and Scientific Notes • Previous Articles Next Articles

Isolation, identification and antibiotic sensitivity of the pathogenic Shewanella putrefaciens from the grass carp Ctenopharyngodon idellus

YE Ming-hao, HU Xiu-cai, LÜ Ai-jun^{Corresponding Author}, SUN Jing-feng, LIU Xiao-xue, BAI Jie

Tianjin Key Lab of Aqua-Ecology and Aquaculture, College of Fisheries, Tianjin Agricultural University, Tianjin 300384, China

Received:2018-08-10 Online:2018-12-20 Published:2019-11-12

Abstract

Abstract: A bacterial strain was isolated from the grass carp（Ctenopharyngodon idellus）, and named C0703. The morphological observation, physiological and biochemical characteristics, sequencing of 16S rDNA and phylogenetic tree analysis were carried out in this study. Results showed that the C0703 strain was a rod-shaped, gram-negative bacillus; The isolated strain was positive for oxidase, esculin and nitrate reduction, and negative reaction for lysine decarboxylase and glucose etc. Furthermore, the 16S rDNA sequences were amplified by PCR method, to get fragment size of sequencing 1 409 bp. It had a 98.23% similarity with 16S rDNA sequence of Shewanella putrefaciens ATCC 8071（NR 119141.1）, and clustered into one group with Shewanella putrefaciens（NR 119141.1）, thus the C0703 strain was identified as Shewanella putrefaciens. Animal experiment showed that the isolated strain has pathogenic to grass carp and causeddeath with a 100% rate, which were consistent with the natural symptoms of diseased fishes. The antibiotic susceptibility testing showed that it was sensitive to amoxicillin, meropenem and nalidixic acid etc, but resistant to ampicillin, cefazolin and vancomycin etc. This study firstly reported the isolation and identification of Shewanella putrefaciens from grass carp, which will provide a scientific reference for prevention and treatment in fishes.

Key words: grass carp, Shewanella putrefaciens, 16S rDNA, physiological and biochemical, antibiotic sensitivity test

CLC Number:

S917.1

YE Ming-hao, HU Xiu-cai, LÜ Ai-jun, SUN Jing-feng, LIU Xiao-xue, BAI Jie. Isolation, identification and antibiotic sensitivity of the pathogenic Shewanella putrefaciens from the grass carp Ctenopharyngodon idellus[J]. Journal of Tianjin Agricultural University, 2018, 25(4): 52-57.

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Isolation, identification and antibiotic sensitivity of the pathogenic Shewanella putrefaciens from the grass carp Ctenopharyngodon idellus

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