运输期间维生素浸泡对红鳍东方鲀（Takifugu rubripes）生理生化指标的影响

doi:10.19640/j.cnki.jtau.2020.04.011

Journal of Tianjin Agricultural University ›› 2020, Vol. 27 ›› Issue (4): 56-61.doi: 10.19640/j.cnki.jtau.2020.04.011

• Researches and Scientific Notes • Previous Articles Next Articles

Effects of vitamin bathing on physiological and biochemical parameters in pufferfish, Takifugu rubripes, during transport

Zhao Huiyan^1,2, Wang Qingkui^{1,2,Corresponding Author}, Xing Kezhi^1,2, Chen Lijun^1,2

1. College of Fisheries, Tianjin Agricultural University, Tianjin 300392, China;
2. Tianjin Key Laboratory of Aqua-ecology and Aquaculture, Tianjin 300392, China

Received:2019-09-11 Online:2020-12-31 Published:2020-12-25

Abstract

Abstract: Transport manipulation can cause stress on fish, resulting in surface damage, deterioration of water quality, endocrine disorders, decreased immunity and even death. Vitamins as essential nutrients for the growth of aquatic organisms, play a role in anti-stress and immunity. Oral vitamins（mainly vitamin B, vitamin C）are important for aquatic animals, although oral administration of riboflavin and ascorbic acid contribute to relieve stress, whether these vitamins contribute to relieving fish stress via bathing has not been reported. In this test, riboflavin（10 mg/L）and ascorbic acid phosphate（3.4 g/L）were dissolved in sea water during Takifugu rubripes’s（23.8±3.8）g transport（lasting 30 minutes）. In control group, no vitamin was added in sea water during transport. Physiological and biochemical parameters in blood and tissues were assayed at 1st, 3rd, 5th, 7th day post-transport. The results showed that the total number of white blood cells, lymphocytes, intermediate cells, neutrophils, erythrocytes and platelets were significantly higher in control group than those in vitamin group（P<0.05）; Hepatopancreas superoxide dismutase（SOD）, lysozyme（LZM）, aspartate aminotransferase（AST）, alanine aminotransferase（ALT）, lactic acid（LD）, catalase（CAT）, malonaldehyde（MDA）, glutathione（GSH）, spleen SOD, CAT, and muscle total antioxidant capacity（T-AOC）, MDA in control group increased significantly（P<0.05）, and no significant difference was observed in vitamin group; Muscle LZM and LDH in both groups varied significantly（P<0.05）. In addition, muscle LZM and LDH in control group varied greater than those in vitamin group. In general, the physiological and biochemical parameters changes in control group after transport were obviously greater than those in vitamin group. In conclusion, bathing with riboflavin and ascorbic acid phosphate during 30-minute transport contributed to relieving transport stress of pufferfish, Takifugu rubripes, which provides a reference for its practicle transport.

Key words: Takifugu rubripes, transport stress, vitamin, physiological and biochemical parameters

CLC Number:

S981.13

Zhao Huiyan, Wang Qingkui, Xing Kezhi, Chen Lijun. Effects of vitamin bathing on physiological and biochemical parameters in pufferfish, Takifugu rubripes, during transport[J]. Journal of Tianjin Agricultural University, 2020, 27(4): 56-61.

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Effects of vitamin bathing on physiological and biochemical parameters in pufferfish, Takifugu rubripes, during transport

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