番茄胚性愈伤组织诱导与增殖研究

doi:10.19640/j.cnki.jtau.2020.03.005

Journal of Tianjin Agricultural University ›› 2020, Vol. 27 ›› Issue (3): 22-25.doi: 10.19640/j.cnki.jtau.2020.03.005

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Study on embryogenic callus induction and proliferation of tomato

XU Ding-fan, LIU Yan-jun^{Corresponding Author}, TAO Ze-man, XIAN Rui, FAN Li-na, YU Yi-han, XU Jiu, YANG Jing-hui

College of Horticulture and Landscape, Tianjin Agricultural University, Tianjin 300384, China

Received:2020-03-22 Online:2020-10-10 Published:2020-10-10

Abstract

Abstract: In order to establish the propagation system of tomato embryogenic callus, the embryogenic callus was induced by inoculating tomato seed embryo on different media, and the induced embryogenic callus was transferred to MS media containing different hormones for propagation culture. The results showed that embryogenic callus could be induced directly on MS+ 5.0 mg/L BA+ 3.0 mg/L 2,4-D+ 0.5 mg/L NAA medium containing 6% sucrose, and embryogenic callus could be maintained best and grew fastest on MS+2.0 mg/L BA+ 0.5 mg/L 2,4-D+30 g/L sucrose medium. The results of this experiment have certain reference value for the development of tomato artificial seeds.

Key words: tomato, embryogenic callus, zygotid embryo, artificial seed

CLC Number:

S641.2

XU Ding-fan, LIU Yan-jun, TAO Ze-man, XIAN Rui, FAN Li-na, YU Yi-han, XU Jiu, YANG Jing-hui. Study on embryogenic callus induction and proliferation of tomato[J]. Journal of Tianjin Agricultural University, 2020, 27(3): 22-25.

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Study on embryogenic callus induction and proliferation of tomato

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