不同外植体与激素对北美海棠松散型愈伤组织诱导的影响

doi:10.19640/j.cnki.jtau.2020.02.007

Journal of Tianjin Agricultural University ›› 2020, Vol. 27 ›› Issue (2): 31-34.doi: 10.19640/j.cnki.jtau.2020.02.007

• Researches and Scientific Notes • Previous Articles Next Articles

Effects of different explants and hormones on the induction of loose callus of North American begonia

ZHANG Wei-long¹, ZHANG Chao², YANG Jing-hui¹, LIU Yan-jun^{1,Corresponding Author}, LI Rui-xiang³, LIU Shan⁴, WANG Zhi-xue⁵

1. College of Horticulture and Landscape, Tianjin Agricultural University, Tianjin 300384, China;
2. Tianjin Shuofang Green Technology Development Co., Ltd, Tianjin 300384, China;
3. Yangjiabo Town Agricultural Service Center, Binhai New Area, Tianjin 300480, China;
4. Tianjin Early Warning Center, Tianjin 300074, China;
5. Fruit Tree Research Institute of Tianjin Academy of Agricultural Science, Tianjin 300384, China

Received:2019-12-16 Online:2020-06-30 Published:2020-07-03

Abstract

Abstract: In order to establish a callus induction system of North American begonia, the leaves, stems and petioles of North American begonia tissue culture seedlings were used as explants, and callus induction was carried out on medium containing different concentrations of 2,4-D. Loose callus induction was performed on medium with different concentrations of 2,4-D, BA, and GA₃. The results showed that the callus induction rate of petiole explants was 100.0%; the callus growth was the fastest on 2,4-D medium with concentration of 2.0 mg/L. And on MS+30.0 g/L sucrose+100.0 mg/L Vc+1.0 mg/L 2,4-D+0.5 mg/L BA+1.0 mg/L GA₃, the callus with loose structure and fastest growth rate can be induced.

Key words: North American begonia, explants, hormone, callus

CLC Number:

S661.4

ZHANG Wei-long, ZHANG Chao, YANG Jing-hui, LIU Yan-jun, LI Rui-xiang, LIU Shan, WANG Zhi-xue. Effects of different explants and hormones on the induction of loose callus of North American begonia[J]. Journal of Tianjin Agricultural University, 2020, 27(2): 31-34.

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Effects of different explants and hormones on the induction of loose callus of North American begonia

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