红掌松散型胚性愈伤组织的诱导

doi:10.19640/j.cnki.jtau.2021.03.005

Journal of Tianjin Agricultural University ›› 2021, Vol. 28 ›› Issue (3): 22-25.doi: 10.19640/j.cnki.jtau.2021.03.005

• Researches and Scientific Notes • Previous Articles Next Articles

Induction of loose embryogenic callus from Anthurium andraeanum

Xu Dingfan, Liu Yanjun^{Corresponding Author}, Bian Yidi, Dong Hongyu

College of Horticulture and Landscape, Tianjin Agricultural University, Tianjin 300392, China

Received:2020-04-30 Online:2021-09-30 Published:2021-10-07

Abstract

Abstract: In order to obtain the loose embryogenic callus of Anthurium andraeanum, different explants, different hormone treatment combinations and MS medium inorganic salt multiple were used to induce the embryogenic callus of Anthurium andraeanum. The results showed that the leaves of Anthurium andraeanum were the best explants for callus induction, and the loose callus could be induced on the medium of 1/2MS+2.0 mg/L 2,4-D+0.2 mg/L NAA, and transferred to MS+1.0 mg/L BA+1.0 mg/L 2,4-D medium, subcultured once every 15 days, and subcultured 2-3 times, and then the loose embryogenic callus of Anthurium andraeanum could be obtained and regenerated directly on MS medium. The induction system of loose embryogenic callus of Anthurium andraeanum was established in this experiment, which can lay a foundation for the study of tissue culture and in vitro mutagenesis of Anthurium andraeanum.

Key words: Anthurium andraean, embryogenic callus, explant, hormone, inorganic salt

CLC Number:

S682.14

Xu Dingfan, Liu Yanjun, Bian Yidi, Dong Hongyu. Induction of loose embryogenic callus from Anthurium andraeanum[J]. Journal of Tianjin Agricultural University, 2021, 28(3): 22-25.

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Induction of loose embryogenic callus from Anthurium andraeanum

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