大麦成熟胚愈伤组织诱导、继代及分化研究

doi:10.19640/j.cnki.jtau.2019.02.002

Journal of Tianjin Agricultural University ›› 2019, Vol. 26 ›› Issue (2): 8-12.doi: 10.19640/j.cnki.jtau.2019.02.002

• Researches and Scientific Notes • Previous Articles Next Articles

Study on callus induction, subculture and differentiation of mature embryo of barley

LI Chen, XIE Xiao-dong, WANG Jing-tai, CUI Chun-yang, WANG Wan-kun, CHEN Xiao-qiang

College of Agronomy and Resource Environment, Tianjin Agricultural University, Tianjin 300384, China

Received:2019-03-06 Online:2019-06-20 Published:2019-09-19

Abstract

Abstract: In this study, G1614 was used as plant materials. The effects of different concentrations of 2,4-D, different kinds and concentrations of carbon source, and different cutting ways to mature embryos on the callus induction of mature embryos from barley G1614 were studied with MS medium as basic medium. And the effects of different concentrations of KT and NAA on the differentiation of barley callus were also studied. The results showed that inducing rate of callus were higher at 5 mg/L 2,4-D. Callus induction rate of mature barley embryos using 30 g/L sucrose as carbon source was higher. The induction rate of barley callus was promoted by longitudinal cut of mature embryo. The addition of 0.5 mg/L NAA and 4.0 mg/L KT in the differentiation medium resulted in a higher differentiation rate of G1614 callus.

Key words: barley, mature embryo, callus, differentiation

CLC Number:

S512.3

LI Chen, XIE Xiao-dong, WANG Jing-tai, CUI Chun-yang, WANG Wan-kun, CHEN Xiao-qiang. Study on callus induction, subculture and differentiation of mature embryo of barley[J]. Journal of Tianjin Agricultural University, 2019, 26(2): 8-12.

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Study on callus induction, subculture and differentiation of mature embryo of barley

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