青睐苜蓿耐盐突变体愈伤组织过氧化物同工酶特性分析

doi:10.19640/j.cnki.jtau.2018.03.006

Journal of Tianjin Agricultural University ›› 2018, Vol. 25 ›› Issue (3): 29-33.doi: 10.19640/j.cnki.jtau.2018.03.006

• Researches and Scientific Notes • Previous Articles Next Articles

Analysis of peroxidase isoenzymes characteristics in salt-tolerant callus of alfalfa mutants

YANG Jing-hui^1a,1b, ZHOU Yi-ni^1a, HUANG Wei-zi^1a, LIU Yan-jun^1a,1b, WU Nan^1c, LU Yun-hui²

1. Tianjin Agricultural University a. College of Horticulture and Landscape, b. Engineering Technology Center for Plant Tissue Culture and Mutation Breeding, c. College of Engineering and Technology, Tianjin 300384, China;
2. Tianjin LÜYIN Landscape and Ecology Construction Co., Ltd, Tianjin 300384, China

Received:2017-03-24 Online:2018-09-20 Published:2019-11-12

Abstract

Abstract: Alfalfa is a kind of high economic value crop. In order to understand the differences between salt-tolerant mutant callus (ST mutant) and common variety callus (CK) of alfalfa, the characteristics of peroxidase isoenzymes type and the content of isozyme were compared between the CK and ST mutant in two development stages of the mutant callus. The results showed that there were seven zymogram bands(p1-p7) in the CK, while there were only five zymogram bands in ST mutant with lack of two bands in Rf 0.430 and Rf 0.733. The similarity coefficient of the zymogram was 0.833. The chromaticity and band width of the main isoenzyme bands p1 (Rf 0.147), p2 (Rf 0.263) and p7 (Rf 0.864) of green ST mutant callus were similar with that of CK. It indicated that their major genes were consistent. The analysis of isoenzyme activity showed that the isoenzyme content of yellow ST mutant callus was the lowest in 5 common isoenzyme bands except for p2 band. The content of p1 and p3 bands with short mobility was the highest in CK, while the content of p4 and p7 with large mobility was the highest in green ST mutant. The total content of isoenzymes was the highest (56.645 0 mg/mL) in CK, green ST Mutant was in the midium (49.653 0 mg/mL) and yellow ST mutant was the least in (42.806 0 mg/mL). In conclusion, the less isoenzyme bands of ST mutant indicated a variation in the genetic background.

Key words: alfalfa, salt tolerance, mutant, callus, peroxidase isoenzymes

CLC Number:

S541.9

YANG Jing-hui, ZHOU Yi-ni, HUANG Wei-zi, LIU Yan-jun, WU Nan, LU Yun-hui. Analysis of peroxidase isoenzymes characteristics in salt-tolerant callus of alfalfa mutants[J]. Journal of Tianjin Agricultural University, 2018, 25(3): 29-33.

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References

[1] 耿华珠. 中国苜蓿[M]. 北京:中国农业出版社,1995.
[2] 孙启忠,玉柱,徐春城. 我国苜蓿产业亟待振兴[J]. 草业科学,2012,29(2):314–319.
[3] 杨宝灵,姜健,封德全,等. 紫花苜蓿耐盐突变体筛选[J]. 大连民族学院学报,2006,8(3):40–43.
[4] 何士敏,赫延龄. 石化空气污染树木叶片内过氧化物酶同工酶的变化[J]. 齐齐哈尔师范学院学报(自然科学版),1992,12(1):40–42.
[5] Weeden N F,Lamb R C.Genetics and linkage analysis of 19 isozyme loci in apple[J]. J Am Soc Hort Sci,1987,112:865-872.
[6] 王振英,祁忠占,杨吉吉,等. 低温胁迫下植物体内POD、COD、ATPase同工酶的变化[J]. 南开大学学报(自然科学版),1996,29(3):29–34.
[7] 雷泞菲,苏智先,陈劲松. 同工酶技术在植物研究中的应用[J] . 四川师范学院学报(自然科学版),2000,21(4):321-325.
[8] 王艇,苏应娟,张宏达. 部分裸子植物的五种同工酶分析[J]. 中山大学学报, 1996,35(6):122-124.
[9] Staub J E, Box J,Meglic V,et al.Comparision of isozyme and random amplified polymorphic DNA data for determining intraspecific variation in Cucumis[J]. Genet Res Crop Evol,1997,44:257-269 .
[10] 李学强,李秀珍,王祥. 5种樱桃属植物的POD、CAT和SOD同工酶分析[J]. 生物学通报,2010,45(2):46-49.
[11] 李珍. 荞麦子叶期过氧化物同工酶研究[J]. 阴山学刊(自然科学版),2017,31(2):55-57.
[12] 张淑改,薛俊杰,张芸香. 松科四种植物的酯酶同工酶分析[J]. 山西农业大学学报,1998,18(2):108-109.
[13] 邹春静,盛晓峰,韩文卿,等. 同工酶分析技术及其在植物研究中的应用[J]. 生态学杂志,2003,22(6):63-69.
[14] 陈一舞,常汝镇,邵桂花,等. 盐胁迫下大豆超氧化物歧化酶的变化[J]. 作物学报,1994,20(3):363-367.
[15] 靳月华,杜英君.超氧化歧酶(SOD)与柳树分布相关性的研究[J] . 生态学杂志,1989,8(5):13-15 .
[16] 陆晓民,孙锦,郭世荣,等. 低氧胁迫下24-表油菜素内酯对黄瓜幼苗根系生长及其无氧呼吸同工酶表达的影响[J]. 生态学杂志,2011,30(11):2497-2502.
[17] 郭秀林,刘子会,王云,等. 张杂谷苹果酸脱氢酶和过氧化物酶同工酶研究[J]. 西北植物学报,2012,32(9):1768-1773.
[18] 王振英,郑坚瑜. 盐胁迫下耐盐与盐敏感水稻的RAPD和POD同工酶检测[J]. 应用与环境生物学报,2000,6(2):106-111.
[19] 李军. 不同地理种源诸葛菜的POD同工酶分析[J]. 西南农业大学学报,1998,20(3):223-225.
[20] 杨静慧,秦艳筠,刘婷,等. 两种愈伤组织生理生化特性和形态结构比较[J]. 天津农业科学,2014,20(8):22-24.
[21] 张东向,张磊,王蕊. 黄岑过氧化物同工酶几种酶液提取方法的比较[J]. 北方园艺,2008(6):33-35.
[22] 陈燕珍,胡丽莲,陶毅明,等. 不同生育期菊芋叶片过氧化物酶同工酶表达特点的研究[J]. 生物学杂志,2007,24(1):25-27.

Analysis of peroxidase isoenzymes characteristics in salt-tolerant callus of alfalfa mutants

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