组织培养条件优化对龙石槲多糖含量的影响

Journal of Tianjin Agricultural University ›› 2015, Vol. 22 ›› Issue (3): 32-35.

• Orginal Article • Previous Articles Next Articles

Effects of Tissue Culture Conditions on Polysaccharide Contents of Dendrobium Draconis

LIU Yan, CHEN Xiao-qiang^*, WANG Xiao-hong, DENG Lin-xia, ZHANG Ya-he, WANG Jing-tai, WANG Dong-yuan

College of Agronomy and Resource Environment, Tianjin Agricultural University, Tianjin 300384, China

Received:2015-03-04 Published:2015-09-30

Abstract

Abstract: By tissue culture techniques, the various culture conditions (different concentrations of 6-BA, NAA, sucrose content and dark time) were adjusted to measure the weight and the content of polysaccharide from Dendrobium draconis at different culture state in this experiment in order to obtain the optimum culture conditions in which more polysaccharide contents of Dendrobium draconis were achieved. The results show that the optimum growth medium for Dendrobium draconis is 1/2 MS, 1.0 mg·L^-1 6-BA, 0.3 mg·L^-1 NAA, 6.5 g·L^-1 agar and 25 g·L^-1 sucrose. The highest polysaccharide content of Dendrobium draconis is the one which is continued to be cultured 7 days after 36 days of dark treatment.

Key words: Dendrobium draconis, polysaccharide, tissue culture

CLC Number:

LIU Yan, CHEN Xiao-qiang, WANG Xiao-hong, DENG Lin-xia, ZHANG Ya-he, WANG Jing-tai, WANG Dong-yuan. Effects of Tissue Culture Conditions on Polysaccharide Contents of Dendrobium Draconis[J]. Journal of Tianjin Agricultural University, 2015, 22(3): 32-35.

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Effects of Tissue Culture Conditions on Polysaccharide Contents of Dendrobium Draconis

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