油用牡丹组培快繁研究

doi:10.19640/j.cnki.jtau.2021.04.005

Journal of Tianjin Agricultural University ›› 2021, Vol. 28 ›› Issue (4): 19-22.doi: 10.19640/j.cnki.jtau.2021.04.005

• Researches and Scientific Notes • Previous Articles Next Articles

Study on tissue culture and rapid propagation of Paeonia ostii

Ren Xiaoqin¹, YANG Jinghui^{1,Corresponding Author}, Liu Yanjun¹, Zhang Jingxin², Ma Xin², Feng Guohua³

1. College of Horticulture and Landscape, Tianjin Agricultural University, Tianjin 300392, China;
2. Tianjin Jizhou Forestry Bureau, Tianjin 301900, China;
3. Tianjin Highway Bureau Direct Branch, Tianjin 300074, China

Received:2019-09-24 Online:2021-12-31 Published:2022-01-05

Abstract

Abstract: In order to establish the rapid propagation system of Paeonia ostii tissue culture, this study used Paeonia ostii axillary buds as explants and used disinfectant of different concentrations for surface disinfection to obtain bacteria-free seedlings.The results showed that the best disinfection effect was obtained when 15% antihuman was used as disinfectant for 5min. In MS+1.0 mg/L BA+0.5 mg/L IAA+5 mg/L AgNO₃ medium, the regeneration bud proliferation effect of Paeonia ostii was the best.The best rooting effect was observed in MS+0.1 mg/L NAA+0.5 g/L AC medium. The results of this experiment can lay a foundation for the study of tissue culture of Paeonia ostia.

Key words: Paeonia ostii, tissue culture, axillary bud, MS culture medium

CLC Number:

S688.9

Ren Xiaoqin, YANG Jinghui, Liu Yanjun, Zhang Jingxin, Ma Xin, Feng Guohua. Study on tissue culture and rapid propagation of Paeonia ostii[J]. Journal of Tianjin Agricultural University, 2021, 28(4): 19-22.

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URL: http://xuebao.tjau.edu.cn/EN/10.19640/j.cnki.jtau.2021.04.005

http://xuebao.tjau.edu.cn/EN/Y2021/V28/I4/19

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Study on tissue culture and rapid propagation of Paeonia ostii

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