ZT和IBA浓度对5个蓝莓品种离体增殖的影响

doi:10.19640/j.cnki.jtau.2020.03.003

Journal of Tianjin Agricultural University ›› 2020, Vol. 27 ›› Issue (3): 14-17.doi: 10.19640/j.cnki.jtau.2020.03.003

• Researches and Scientific Notes • Previous Articles Next Articles

Effect of concentrations of ZT and IBA on multiplication in vitro of five blueberry varieties

YE Xia, SUN Shi-hai^{Corresponding Author}, GUO Ru, HUA Ren-rui

College of Horticulture and Landscape, Tianjin Agricultural University, Tianjin 300384, China

Received:2020-02-28 Online:2020-10-10 Published:2020-10-10

Abstract

Abstract: The effects of different concentrations of ZT and IBA on the multiplication of 5 blueberry varieties（Bluecrop, Earliblue, Chippewa, Misty and Sharpblue）seedlings in vitro were studied by using the stem segments in vitro of 5 blueberry varieties as materials. The results showed that the different varieties of blueberry had different appropriate ZT and IBA concentrations for seedling multiplication in vitro when they were cultured in WPM medium. Adding ZT 1.0 mg/L had the best effect on the multiplication of Bluecrop, with a multiplication coefficient of 3.41 and plant height of 3.0 cm. Adding ZT 1.5 mg/L had the best effect on multiplication of Earliblue, with a multiplication coefficient of 3.6 and plant height of 4.1cm. Adding ZT 1.5 mg/L+IBA 0.05 mg/L had the best effect on multiplication of Misty, with a multiplication coefficient of 2.71 and plant height of 4.8 cm. Adding ZT 2.0 mg/L+IBA 0.05 mg/L had the best effect on the multiplication of Chippewa and Sharpblue, with multiplication coefficients of 4.3 and 6.7, respectively, and plant height of 4.9 cm and 3.5 cm, respectively. Through comprehensive analysis, the optimal medium was WPM+ZT 1.0 mg/L for Bluecrop stem multiplication in vitro, WPM+ZT 1.5 mg/L for Earliblue, WPM+ZT 1.5 mg/L+IBA 0.05 mg/L for Misty and WPM+ZT 2.0 mg/L+IBA 0.05 mg/L for Chippewa and Sharpblue.

Key words: blueberry, ZT, IBA, multiplication coefficient

CLC Number:

S663.9

YE Xia, SUN Shi-hai, GUO Ru, HUA Ren-rui. Effect of concentrations of ZT and IBA on multiplication in vitro of five blueberry varieties[J]. Journal of Tianjin Agricultural University, 2020, 27(3): 14-17.

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: http://xuebao.tjau.edu.cn/EN/10.19640/j.cnki.jtau.2020.03.003

http://xuebao.tjau.edu.cn/EN/Y2020/V27/I3/14

References

[1] 王连润,马钧,胡忠荣,等. 蓝莓离体快速繁殖研究[J]. 红河学院学报,2009,7(5):16-18.
[2] 杨喜平,张胜利,王琚钢,等. 我国蓝莓研究[J]. 内蒙林业科技,2012,38(3):55-58.
[3] 汤伟华. 蓝莓茎段初代培养技术[J]. 湖北农业科学,2012,51(6):1263-1264,1267.
[4] 张帅,谷晓平,莫建国,等. 基于GIS的贵州蓝莓生态适宜性区划[J]. 贵州农业科学,2014(10):255-257.
[5] 蒋鑫,杨静慧,何心,等. 温室与大棚中不同蓝莓品种的生长生理特性比较[J]. 天津农学院学报,2020,27(1):15-20.
[6] 申岩. 蓝莓露地栽培技术[J]. 天津农业科学,2009(3):62-64.
[7] 吕晨菲,杨静慧,秦艳筠,等. 促成栽培的蓝莓品种果实外观特性比较[J]. 天津农学院学报,2019,26(2):27-30,34.
[8] 郑理乔,黄成林,刘华,等. 兔眼蓝莓组织培养过程中褐化与生根问题的探讨[J]. 安徽农业大学学报,2012,39(5):777-782.
[9] 朱宏芬,沈岚,黄坚,等. 兔眼蓝莓“灿烂”组织培养与植株再生研究[J]. 北方园艺,2012(19):105-107.
[10] 李丽容,金开正. 兔眼蓝莓组培快繁试验[J]. 中国南方果树,2010,39(1):71-72.
[11] 廉家盛,朴炫春,廉美兰,等. 培养基种类、玉米素浓度及pH值对蓝莓“美登”组培增殖生长的影响[J]. 延边大学农学学报,2010,32(4):269-272.
[12] 肖海峻,庞俊兰,孟利前,等. 不同基因型蓝莓离体茎段的增殖培养技术[J]. 分子植物育种,2018,16(8):2587-2592.
[13] 饶宝蓉,陈泳和,江文清,等. 蓝莓夏普蓝组培苗繁殖技术研究[J]. 江西农业学报,2014,26(10):46-49.
[14] 龚雪元,杜亚填,张翔宇,等. 南高丛蓝莓快繁技术体系研究[J]. 植物研究,2014,34(1):131-135.
[15] 史文君,赵永钦,王培培,等. 北高丛蓝莓茎段离体培养及增殖技术[J]. 经济林研究,2012,30(2):56-60.

Effect of concentrations of ZT and IBA on multiplication in vitro of five blueberry varieties

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	Wang Zhenli, Wang Junbin, Wang Min, Yang Zongfen, Wang Haifeng. Study on extraction optimization of polyphenols from Mesembryanthemum crystallinum and its antioxidant and bacteriostatic activities [J]. Journal of Tianjin Agricultural University, 2022, 29(3): 23-28.
[2]	Zhang Weilong, Zhang Banban, Yang Jinghui, Liu Yanjun, Zhang Chao, Feng Guohua. Yellowing rule of blueberries in different periods and the relationship between growth and yellowing [J]. Journal of Tianjin Agricultural University, 2022, 29(1): 24-27.
[3]	Yang Yueyan, Mo Yihao, Tian Jiamin, Jin Xiang, Jia Liming, Pu Lei. Antibacterial activity of Earthworm homogenate supernatant on common harmful bacteria in pig farm [J]. Journal of Tianjin Agricultural University, 2021, 28(3): 75-80.
[4]	Tian Jiamin, Mo Yihao, Yang Yueyan, Jin Xiang, Dai Yuxing, Zhang Jianbin, Jia Jinghua, Pu Lei, Guo Liang. A comparative study on the bacteriostatic effects of earthworm products with different treatments on harmful bacteria from pig farms [J]. Journal of Tianjin Agricultural University, 2021, 28(2): 43-46.
[5]	Zhang Weilong, Huang Handa, Yang Jinghui, Wu Chunxia, Liang Fahui, Shi Yanyu. Changes of blueberry fruit quality at four storage temperatures [J]. Journal of Tianjin Agricultural University, 2020, 27(4): 27-34.
[6]	MENG Jie, HUANG Han-da, YANG Jing-hui, LIU Yan-jun, LIANG Fa-hui, ZHANG Chao. Quality differences and changes of fruit stored in room temperature between different blueberry varieties [J]. Journal of Tianjin Agricultural University, 2020, 27(2): 11-17.
[7]	JIANG Xin, HUANG Han-da, YANG Jing-hui, FENG Guo-hua, WANG Xing, HU Zhong-hui. Comparative study on quality changes of different varieties of blueberry promoted by cultivation [J]. Journal of Tianjin Agricultural University, 2019, 26(4): 9-15.
[8]	LÜ Chen-fei, WANG Mao-si, HUANG Wei-zi, YANG Jing-hui, LIU Yan-jun, HUANG Jun-xuan. Establishment and optimization of two-dimensional electrophoresis system for proteins in dormant flower-bud of blueberry [J]. Journal of Tianjin Agricultural University, 2019, 26(4): 16-19.
[9]	HUANG Yan, YANG Jing-hui, HUANG Han-da, PANG Zhi-rui, WANG Wei, LIU Yan-jun, WANG Zhi-xue. Comparison of morphological characteristics of different varieties of blueberry flowers in greenhouse [J]. Journal of Tianjin Agricultural University, 2019, 26(2): 18-21.
[10]	LÜ Chen-fei, YANG Jing-hui, QIN Yan-jun, WANG Mao-si, ZHANG Ban-ban. Differences of fruit external quality among varieties of blueberry under the forcing cultivation in greenhouse [J]. Journal of Tianjin Agricultural University, 2019, 26(2): 27-30.
[11]	CHAI Ci-jiang, WANG Kun-ying, ZHANG Wen-wen, LIU Chun-peng, WEI Wei, ZOU Hang. Effect of sterilizing method and concentration of ZT on blueberry plantlet in Vitro proliferation [J]. Journal of Tianjin Agricultural University, 2019, 26(1): 1-5.
[12]	JIANG Xin, YANG Jing-hui, HE Xin, WANG Mao-si, LI Jia-yi, JI Xin-ning, SONG Ke. Growth and physiological characteristics of blueberry among different cultivars in plastic greenhouses and artificial heating greenhouses [J]. Journal of Tianjin Agricultural University, 2019, 26(1): 26-29.
[13]	YANG Jing-hui, TANG Yu-qi, HUANG Han-da, WANG Mao-si, JI Xin-ning, LI Jia-yi. Temperature control and corresponding cultivation techniques of blueberry forcing culture in sunlight greenhouse [J]. Journal of Tianjin Agricultural University, 2018, 25(4): 29-32.
[14]	SONG Ke, YANG Jing-hui, XIA Kai-li, LIU Yan-jun, LIANG Fa-hui, ZHANG Wei-hua. Differences in ultrastructure of floral organs between different blueberry varieties [J]. Journal of Tianjin Agricultural University, 2018, 25(2): 20-23.
[15]	YANG Jing-hui, JI Liu-yang, HUANG Han-da, WANG Wei, ZHANG Jing-xin, FENG Yan. Influence of different pretreatment methods for blueberry seed germination [J]. Journal of Tianjin Agricultural University, 2018, 25(1): 25-28.