蓝莓休眠花芽蛋白质双向电泳体系的建立和优化

doi:10.19640/j.cnki.jtau.2019.04.004

Journal of Tianjin Agricultural University ›› 2019, Vol. 26 ›› Issue (4): 16-19.doi: 10.19640/j.cnki.jtau.2019.04.004

• Researches and Scientific Notes • Previous Articles Next Articles

Establishment and optimization of two-dimensional electrophoresis system for proteins in dormant flower-bud of blueberry

LÜ Chen-fei, WANG Mao-si, HUANG Wei-zi, YANG Jing-hui^{Corresponding Author}, LIU Yan-jun, HUANG Jun-xuan

College of Horticulture and Landscape, Tianjin Agricultural University, Tianjin 300384, China

Received:2019-05-05 Published:2020-01-19

Abstract

Abstract: In order to establish a protein two-dimensional electrophoresis system suitable for blueberry flower -bud, taking the dormantflower bud of 'Brigita', the main blueberry variety in Jizhou District of Tianjin as the test material, selectingfive protein samples volumes（2.00, 2.25, 2.50, 2.75, 3.00 mg）, two focusing voltages（8 000, 10 000 V）and three focusing times（6, 7, 8 h）for two-dimensional electrophoresis,the chromatographic patterns after R-250 Coomassie brilliant blue dyeingwere compared and analyzed.. The results showed that, when the sample volumes was 3.00 mg, the focusing voltage was 8000 V and the focusing time was 8 h, which can obtain a high resolution electrophoresis map with a large number of protein spots. A two- dimensional electrophoresis system suitable for blueberry dormant flower bud protein was established, which laysa foundation for the development of blueberry proteomics analysis, and providestheoretical basis and technical support for the study of molecular mechanism of blueberry flower bud dormancy and dormancy release.

Key words: blueberry, flower bud, protein, two-dimensional electrophoresis

CLC Number:

S667.201

LÜ Chen-fei, WANG Mao-si, HUANG Wei-zi, YANG Jing-hui, LIU Yan-jun, HUANG Jun-xuan. Establishment and optimization of two-dimensional electrophoresis system for proteins in dormant flower-bud of blueberry[J]. Journal of Tianjin Agricultural University, 2019, 26(4): 16-19.

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Establishment and optimization of two-dimensional electrophoresis system for proteins in dormant flower-bud of blueberry

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