不同处理的蚯蚓产物对猪场源有害菌抑菌效果的对比研究

doi:10.19640/j.cnki.jtau.2021.02.009

Journal of Tianjin Agricultural University ›› 2021, Vol. 28 ›› Issue (2): 43-46.doi: 10.19640/j.cnki.jtau.2021.02.009

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A comparative study on the bacteriostatic effects of earthworm products with different treatments on harmful bacteria from pig farms

Tian Jiamin¹, Mo Yihao¹, Yang Yueyan¹, Jin Xiang¹, Dai Yuxing¹, Zhang Jianbin¹, Jia Jinghua², Pu Lei^1,*, Guo Liang^1,*

1. Tianjin Key Laboratory of Agricultural Animal Breeding and Healthy Husbandry, College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin 300392, China;
2. Tianjin Minsheng Bioengineering Co., Ltd., Tianjin 301500, China

Received:2019-10-21 Published:2021-07-26

Abstract

Abstract: In order to study the antibacterial effect of different treatments of earthworm on several kinds of harmful bacteria in pig farm, it was applied to the feed additive of pig. In this experiment, with the paper diffusion method and ultraviolet spectrophotometry, the body cavity fluid obtained by electric shock of earthworm, and the body cavity fluid obtained by electric shock of earthworm after induction were compared to analyze the bacteriostatic performance on escherichia coli, staphylococcus aureus and streptococcus suis. Through the paper diffusion method, it was found that only earthworm homogenate group 2∶1 had bacteriostatic effect on escherichia coli. Earthworm homogenate in groups 2∶1 and 4∶5, earthworm electroshock treatment group and induced shock treatment group all had antibacterial effects on staphylococcus aureus. Only earthworm homogenate group 4∶5 had antibacterial effects on streptococcus suis. By spectrophotometric comparison, it was found that earthworm homogenate and the electroshock treatment group had no significant inhibitory effect. Earthworm electroshock treatment group could significantly inhibit the growth of staphylococcus (P<0.005), while earthworm homogenate group had no significant inhibitory effect. Earthworm homogenate and the electroshock treatment group had no obvious inhibitory effect on streptococcus suis from big farm, and even the OD value increased significantly at 6 h. The research on the antibacterial properties of earthworm on harmful bacteria in pig farms provides the basis for the study of non-resistant and substitute resistant feed.

Key words: daping no.2 earthworm, homograte supermatan, supernatant of electric shock, induced shock supernatant, pig farms harmful baceria, antibacterial properies

CLC Number:

Tian Jiamin, Mo Yihao, Yang Yueyan, Jin Xiang, Dai Yuxing, Zhang Jianbin, Jia Jinghua, Pu Lei, Guo Liang. A comparative study on the bacteriostatic effects of earthworm products with different treatments on harmful bacteria from pig farms[J]. Journal of Tianjin Agricultural University, 2021, 28(2): 43-46.

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A comparative study on the bacteriostatic effects of earthworm products with different treatments on harmful bacteria from pig farms

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