依赖SRP对大肠杆菌膜蛋白定位的研究

doi:10.19640/j.cnki.jtau.2021.03.013

Journal of Tianjin Agricultural University ›› 2021, Vol. 28 ›› Issue (3): 61-64.doi: 10.19640/j.cnki.jtau.2021.03.013

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Localization of E.coli membrane proteins dependent on SRP

Cui Boxian¹, Zhao Liuqun², Zhao Sheng¹, Yang Jiande^{1,Corresponding Author}, Liu Yanfei¹, Zhang Dawei^{2,Corresponding Author}

1. College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin 300392, China;
2. Tianjin Institute of Industrial Biotechnology, Chinese Academy of Science, Tianjin 300380, China

Received:2019-10-22 Online:2021-09-30 Published:2021-10-07

Abstract

Abstract: In Escherichia coli, signal recognition particle（SRP）recognizes and binds signal peptides being translated by ribosome to guide the new peptide chain to the cell membrane. In this experiment, localization of E.coli membrance protein in HDB51 and MG1655 was studied by Western blot based on biotinylation for membrane proteins targeting. By comparing the presence or absence of SRP in different E.coli, the substrate protein FtsQ that depended on SRP in the strain E. coli HDB51 was localized. The location of FtsQ was significantly affected without SRP. This verifies that SRP helps membrane protein localization. It can provide references for the study of localizing E. coli membrane proteins dependent on SRP.

Key words: Escherichia coli, signal recognition particle, membrane protein localization, FtsQ

CLC Number:

Q816

Cui Boxian, Zhao Liuqun, Zhao Sheng, Yang Jiande, Liu Yanfei, Zhang Dawei. Localization of E.coli membrane proteins dependent on SRP[J]. Journal of Tianjin Agricultural University, 2021, 28(3): 61-64.

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Localization of E.coli membrane proteins dependent on SRP

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