Journal of Tianjin Agricultural University ›› 2020, Vol. 27 ›› Issue (2): 44-47.doi: 10.19640/j.cnki.jtau.2020.02.010

• Researches and Scientific Notes • Previous Articles     Next Articles

Clinical signs and histochemical observation of Citrobacter freundii disease in turtle(Trachemys scripta elegans

LUO Fu-li1, NIU Yu-chen2, HU Xiu-cai1, LÜ Ai-jun1,Corresponding Author, YE Ming-hao1, SUN Jing-feng1   

  1. 1. Tianjin Key Laboratory of Aqua-Ecology and Aquaculture, College of Fisheries, Tianjin Agricultural University, Tianjin 300384, China;
    2. School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China
  • Received:2019-05-23 Online:2020-06-30 Published:2020-07-03

Abstract: In order to observe the clinical signs and its histopathological characteristics of Citrobacter freundii disease in turtle Trachemys scripta elegans, Histopathology was studied by artificial infection test, paraffin section and HE staining. The results showed that the main clinical signs of the infected sick turtle were conjunctivitis of the eye, ulceration of the oral and nasal skin; anatomy showed that the lungs were black, the intestinal tract was yellowish, with no content, and the water-like mucus increased. Histopathological observation showed the increase of pigment cells in eyelid epithelium, degeneration, necrosis and exfoliation of gastric and intestinal epithelial cells. The myocardial fibrils were widened, necrotic, and infiltrated with numerous inflammatory cells in the heart tissue. The boundary between pulmonary alveolar cells and vascular endothelial cells was unclear, and the alveolar wall had capillary congestion with Liver cell necrosis, central venous congestion, hepatic cord disorder, renal tubular epithelial cell necrosis, and glomerular hemorrhage. There was a large amount of inflammatory cell infiltration in muscle fiber space. The above results can provide some reference for elucidating the pathogenic mechanism of Citrobacter freundii disease of turtle.

Key words: Trachemys scripta elegans, Citrobacter freundii disease, tissue pathology

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